Tanja Mittag
@tanjamittag.bsky.social
Phase separation and disordered proteins!
It’s actually all your own doing, Alex. Very well deserved.
October 2, 2025 at 7:36 PM
It’s actually all your own doing, Alex. Very well deserved.
Congratulations, Alex!!
September 30, 2025 at 5:42 AM
Congratulations, Alex!!
Lastly, we provide a transferable framework to critically evaluate the functional roles of other phase separating systems.
July 9, 2025 at 4:28 AM
Lastly, we provide a transferable framework to critically evaluate the functional roles of other phase separating systems.
We interpret our data to mean that Gcn4 sequence variants can function as soluble complexes as well as heterotypic condensates but that condensates formed by variants with high affinities can dampen activities. Our study therefore reconciles
opposing views in the literature.
opposing views in the literature.
July 9, 2025 at 4:28 AM
We interpret our data to mean that Gcn4 sequence variants can function as soluble complexes as well as heterotypic condensates but that condensates formed by variants with high affinities can dampen activities. Our study therefore reconciles
opposing views in the literature.
opposing views in the literature.
We find that abilities of Gcn4 variants to co-phase separate and form complexes with Med15 are highly intertwined. Separating the transcriptional functions emerging from condensate or soluble complex formation is therefore not straightforward, and extant literature should be interpreted cautiously.
July 9, 2025 at 4:26 AM
We find that abilities of Gcn4 variants to co-phase separate and form complexes with Med15 are highly intertwined. Separating the transcriptional functions emerging from condensate or soluble complex formation is therefore not straightforward, and extant literature should be interpreted cautiously.
Homotypic phase separation of Gcn4 variants does not
predict in vivo activities well. DNA binding solubilizes Gcn4 condensates, which is reversed by Med15 association. Thus, binding to Mediator may facilitate the formation of Gcn4 condensates even on repetitive regulatory DNA sites in the genome.
predict in vivo activities well. DNA binding solubilizes Gcn4 condensates, which is reversed by Med15 association. Thus, binding to Mediator may facilitate the formation of Gcn4 condensates even on repetitive regulatory DNA sites in the genome.
July 9, 2025 at 4:23 AM
Homotypic phase separation of Gcn4 variants does not
predict in vivo activities well. DNA binding solubilizes Gcn4 condensates, which is reversed by Med15 association. Thus, binding to Mediator may facilitate the formation of Gcn4 condensates even on repetitive regulatory DNA sites in the genome.
predict in vivo activities well. DNA binding solubilizes Gcn4 condensates, which is reversed by Med15 association. Thus, binding to Mediator may facilitate the formation of Gcn4 condensates even on repetitive regulatory DNA sites in the genome.
Given that transcriptional condensates in yeast are expected to
be miniscule and therefore challenging to visualize, and given that even the presence of condensates would not mean that they are the functional entities, we chose a mutagenesis-based approach.
be miniscule and therefore challenging to visualize, and given that even the presence of condensates would not mean that they are the functional entities, we chose a mutagenesis-based approach.
July 9, 2025 at 4:21 AM
Given that transcriptional condensates in yeast are expected to
be miniscule and therefore challenging to visualize, and given that even the presence of condensates would not mean that they are the functional entities, we chose a mutagenesis-based approach.
be miniscule and therefore challenging to visualize, and given that even the presence of condensates would not mean that they are the functional entities, we chose a mutagenesis-based approach.
Phase separation regulates many biological processes, but the role of TF-mediated condensates in gene regulation is contentious. We used Gcn4, a prototypical budding yeast TF, to assess two competing models for transcription activation, i.e., mediated via soluble complexes or through condensates.
July 9, 2025 at 4:16 AM
Phase separation regulates many biological processes, but the role of TF-mediated condensates in gene regulation is contentious. We used Gcn4, a prototypical budding yeast TF, to assess two competing models for transcription activation, i.e., mediated via soluble complexes or through condensates.