Susana Nunes
@susananunes.bsky.social
Scientific Intern in the Chromatin Structure and Rhythms Lab at @ISTA
Previously a bachelor student in the Nadler Lab at @MPI-CBG
Previously a bachelor student in the Nadler Lab at @MPI-CBG
Reposted by Susana Nunes
We have wondered what a complex archaeal cell might look like ever since 2014. It’s been a long road (and the journey is far from over), but it’s a good time to pause for breath and look. These Asgard archaeal cells are a surprise! And that is the joy of being a cell biologist.
November 7, 2025 at 11:02 AM
We have wondered what a complex archaeal cell might look like ever since 2014. It’s been a long road (and the journey is far from over), but it’s a good time to pause for breath and look. These Asgard archaeal cells are a surprise! And that is the joy of being a cell biologist.
Reposted by Susana Nunes
Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
October 28, 2025 at 3:07 PM
Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
Reposted by Susana Nunes
Sometimes we need a moment, but if you tune out completely, they win. Fascism doesn’t need your support, just your silence.
October 10, 2025 at 2:31 AM
Sometimes we need a moment, but if you tune out completely, they win. Fascism doesn’t need your support, just your silence.
Reposted by Susana Nunes
Lol the Nobels can't even acknowledge women's contribution to discovery. But sure let's acknowledge The Machines.
October 9, 2025 at 7:00 PM
Lol the Nobels can't even acknowledge women's contribution to discovery. But sure let's acknowledge The Machines.
Reposted by Susana Nunes
Lipid imaging on the cover of @nature.com! Great times for lipid cell biology indeed. And a fantastic recognition of all the hard work by the team, especially Juan M. Iglesias-Artola and Kristin Böhlig (who made the cover). Link to article: www.nature.com/articles/s41...
October 9, 2025 at 9:01 AM
Lipid imaging on the cover of @nature.com! Great times for lipid cell biology indeed. And a fantastic recognition of all the hard work by the team, especially Juan M. Iglesias-Artola and Kristin Böhlig (who made the cover). Link to article: www.nature.com/articles/s41...
Reposted by Susana Nunes
Cryo-ET visualization of HTT bound to F-actin, highlighted in the yellow square on the left.
On the right, a detailed zoom-in view of the molecular model of the HTT/F-actin complex is shown.
On the right, a detailed zoom-in view of the molecular model of the HTT/F-actin complex is shown.
September 22, 2025 at 1:57 PM
Cryo-ET visualization of HTT bound to F-actin, highlighted in the yellow square on the left.
On the right, a detailed zoom-in view of the molecular model of the HTT/F-actin complex is shown.
On the right, a detailed zoom-in view of the molecular model of the HTT/F-actin complex is shown.
Reposted by Susana Nunes
This is absolutely a must read. We are in deep trouble and I am even less optimistic than the authors.
AI can now generate fake microscopy images that are nearly impossible to detect. A serious threat to scientific integrity—and we’re not prepared for it.
Commentary in @natnano.nature.com
#nanotechnology
Commentary in @natnano.nature.com
#nanotechnology
September 15, 2025 at 11:10 AM
This is absolutely a must read. We are in deep trouble and I am even less optimistic than the authors.
Reposted by Susana Nunes
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
September 13, 2025 at 12:05 AM
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
Reposted by Susana Nunes
First pre-print from the Lucas Lab led by Matthew Giammar and @joshdcryoem.bsky.social online today! We develop a new, extensible Python implementation of 2DTM and apply it to build pixel size optimization, constrained search and characterize molecular motions in situ www.biorxiv.org/content/10.1...
Leopard-EM: An extensible 2DTM package to accelerate in situ structural biology
The ability to generate high-resolution views of cells with cryogenic electron microscopy (cryo-EM) can reveal the molecular mechanisms of biological processes in their native cellular context. The re...
www.biorxiv.org
August 30, 2025 at 12:49 AM
First pre-print from the Lucas Lab led by Matthew Giammar and @joshdcryoem.bsky.social online today! We develop a new, extensible Python implementation of 2DTM and apply it to build pixel size optimization, constrained search and characterize molecular motions in situ www.biorxiv.org/content/10.1...
Reposted by Susana Nunes
Better ML for cryo-ET starts with better benchmarks.
We built a phantom cryo-ET dataset (~500 tomograms) + hosted a Kaggle challenge.
The result: community models beat expert tools.
Read more in the @natmethods.nature.com article that just came out:
🔗 doi.org/10.1038/s415...
We built a phantom cryo-ET dataset (~500 tomograms) + hosted a Kaggle challenge.
The result: community models beat expert tools.
Read more in the @natmethods.nature.com article that just came out:
🔗 doi.org/10.1038/s415...
A realistic phantom dataset for benchmarking cryo-ET data annotation - Nature Methods
A standardized, realistic phantom dataset consisting of ground-truth annotations for six diverse molecular species is provided as a community resource for cryo-electron-tomography algorithm benchmarki...
doi.org
August 26, 2025 at 2:22 PM
Better ML for cryo-ET starts with better benchmarks.
We built a phantom cryo-ET dataset (~500 tomograms) + hosted a Kaggle challenge.
The result: community models beat expert tools.
Read more in the @natmethods.nature.com article that just came out:
🔗 doi.org/10.1038/s415...
We built a phantom cryo-ET dataset (~500 tomograms) + hosted a Kaggle challenge.
The result: community models beat expert tools.
Read more in the @natmethods.nature.com article that just came out:
🔗 doi.org/10.1038/s415...
Reposted by Susana Nunes
If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...
August 23, 2025 at 11:21 PM
If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...
Reposted by Susana Nunes
Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
August 21, 2025 at 5:19 AM
Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Susana Nunes
Today is June 25. On this date in 1903 Marie Curie defended her doctoral thesis on radioactive substances at Université de la Sorbonne in Paris, becoming 1st woman in France to receive a doctoral degree. Image shows the thesis cover.
June 25, 2025 at 6:02 AM
Today is June 25. On this date in 1903 Marie Curie defended her doctoral thesis on radioactive substances at Université de la Sorbonne in Paris, becoming 1st woman in France to receive a doctoral degree. Image shows the thesis cover.
Reposted by Susana Nunes
Portugal, 25 de abril de 1974.
April 25, 2025 at 6:53 AM
Portugal, 25 de abril de 1974.
Reposted by Susana Nunes
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
April 11, 2025 at 8:35 AM
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
Reposted by Susana Nunes
Groundbreaking study in Cell from @leventallab.bsky.social: phospholipid asymmetry is a defining feature of the plasma membrane and cholesterol fills the holes — major implications for how this membrane works. A #lipidtime must-read! www.cell.com/cell/abstrac...
Cell membranes sustain phospholipid imbalance via cholesterol asymmetry
This work challenges a major assumption in cell biology by showing that lipid bilayers
can have drastically different phospholipid abundances between their two leaflets.
This lipid abundance asymmetry...
www.cell.com
April 3, 2025 at 3:07 PM
Groundbreaking study in Cell from @leventallab.bsky.social: phospholipid asymmetry is a defining feature of the plasma membrane and cholesterol fills the holes — major implications for how this membrane works. A #lipidtime must-read! www.cell.com/cell/abstrac...
Reposted by Susana Nunes
I am really excited that the second story of my PhD work is on biorxiv now. If you are into lipids, membrane trafficking and super resolution microscopy check it out! Big shout out also to our collaborators, the Honigmann lab and Modes lab.
Are lipids actively sorted during clathrin mediated endocytosis like proteins? @mathilda95.bsky.social addresses this key open question together with our collaborators from the Honigmann and Modes labs using a new Lipid-STED workflow.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
March 10, 2025 at 9:46 PM
I am really excited that the second story of my PhD work is on biorxiv now. If you are into lipids, membrane trafficking and super resolution microscopy check it out! Big shout out also to our collaborators, the Honigmann lab and Modes lab.
Reposted by Susana Nunes
It’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density, which usually isn’t attainable. Lipid-CLEM brought to you by @mathilda95.bsky.social changes that.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
January 31, 2025 at 10:28 AM
It’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density, which usually isn’t attainable. Lipid-CLEM brought to you by @mathilda95.bsky.social changes that.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Susana Nunes
I'd like to draw your attention to this truly excellent paper from the Taraska lab on cryoET of plasma membrane associated proteins. Everyone who is thinking about probes in the cryoET space should also see what they could do with ferritag (fig 6) www.nature.com/articles/s41...
January 22, 2025 at 4:40 PM
I'd like to draw your attention to this truly excellent paper from the Taraska lab on cryoET of plasma membrane associated proteins. Everyone who is thinking about probes in the cryoET space should also see what they could do with ferritag (fig 6) www.nature.com/articles/s41...
Reposted by Susana Nunes
One of the Earth's greatest treasures of biodiversity is rapidly dying, ignored by politicians, hidden beneath the waves. It's painful, but we must bear witness. Don't look away. 🧪🌏🌊🪸
Silence still from the Australian government. In the meantime, independent scientists are beginning to publish their peer-reviewed data.
For example, One Tree Reef had catastrophic mortality (95%) of branching and tabular staghorn corals in 2024.
aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1...
For example, One Tree Reef had catastrophic mortality (95%) of branching and tabular staghorn corals in 2024.
aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1...
January 21, 2025 at 1:56 AM
One of the Earth's greatest treasures of biodiversity is rapidly dying, ignored by politicians, hidden beneath the waves. It's painful, but we must bear witness. Don't look away. 🧪🌏🌊🪸
Reposted by Susana Nunes
The teams validated the potential of this dataset through subtomogram averaging of 7 macromolecules. All but one of these structures went to sub-nanometer resolution🔍🔬
Check out this nucleosome by @aliciakmichael.bsky.social and Ricardo. The alpha helices of the histones are clearly resolved! 🧪🧶🧬
Check out this nucleosome by @aliciakmichael.bsky.social and Ricardo. The alpha helices of the histones are clearly resolved! 🧪🧶🧬
January 6, 2025 at 11:19 AM
The teams validated the potential of this dataset through subtomogram averaging of 7 macromolecules. All but one of these structures went to sub-nanometer resolution🔍🔬
Check out this nucleosome by @aliciakmichael.bsky.social and Ricardo. The alpha helices of the histones are clearly resolved! 🧪🧶🧬
Check out this nucleosome by @aliciakmichael.bsky.social and Ricardo. The alpha helices of the histones are clearly resolved! 🧪🧶🧬
Reposted by Susana Nunes
Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
January 6, 2025 at 11:19 AM
Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
Reposted by Susana Nunes
Wishing everyone a happy and healthy new year!! 🤗 Excited to start our #ERC StG #ChromaChrono with my amazing 🌟team digging into mechanisms of circadian transcription factor interactions in chromatin ⏰🧬. See our freshly updated lab website: www.chromatin-structure-and-rhythms-lab.com
Home | Alicia K Michael Ph
www.chromatin-structure-and-rhythms-lab.com
January 2, 2025 at 11:05 AM
Wishing everyone a happy and healthy new year!! 🤗 Excited to start our #ERC StG #ChromaChrono with my amazing 🌟team digging into mechanisms of circadian transcription factor interactions in chromatin ⏰🧬. See our freshly updated lab website: www.chromatin-structure-and-rhythms-lab.com
Reposted by Susana Nunes
More than 1'800 tomograms available for the community. Towards visual proteomics using our favorite organism Chlamy!
Check out the preprint: www.biorxiv.org/content/10.1...
Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com
#TeamTomo
Check out the preprint: www.biorxiv.org/content/10.1...
Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com
#TeamTomo
Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii
In situ cryo-electron tomography (cryo-ET) has emerged as the method of choice to investigate structures of biomolecules in their native context. However, challenges remain in the efficient production...
www.biorxiv.org
December 29, 2024 at 11:47 AM
More than 1'800 tomograms available for the community. Towards visual proteomics using our favorite organism Chlamy!
Check out the preprint: www.biorxiv.org/content/10.1...
Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com
#TeamTomo
Check out the preprint: www.biorxiv.org/content/10.1...
Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com
#TeamTomo
Reposted by Susana Nunes
Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii biorxiv.org/cgi/content/short/2024.12.28.630444v1
December 29, 2024 at 8:10 AM
Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii biorxiv.org/cgi/content/short/2024.12.28.630444v1