This was a delightful meeting, thank you @nynkedekkerlab.bsky.social, @achilleskap.bsky.social , & @lumicks.bsky.social . I had a great time meeting UK’s young single molecule enthusiasts, so many exciting posters!

A key insight was building temporal information and memory into the ANN architecture. A huge thanks to all, especially our ‘smart’ (in so many ways) partner @maweigert.bsky. We could now detect local depolarization following constriction, using commonly used (bleachy TMRE) fluorescent biosensors.

Our latest preprint www.biorxiv.org/content/10.1..., describes Willi Stepp’s project to make smart microscopy even gentler by doing event detection in phase contrast. We developed neural networks to detect mito-LD and mito-lysosome contacts, as well as mitochondrial pre-fission constrictions.

Rockin’ out at EMBO in situ with @lycasworks.bsky.social @jonasries.bsky.social Christian Zimmerli, Mark Bates 🤘🔬

But what triggers pearling, and how can we make sense of the distinct morphological changes that take place? We pinpoint several perturbations that differentially impact membrane tension, elasticity, or osmotic pressure, and present a unifying framework: t.ly/yTsPr Collab with Calico Labs.

Nucleoids are known to be regularly spaced along mitochondria, important for their inheritance and maintenance of the local proteome. We identified pearling, a Plateau-Rayleigh type instability described since the 1980s, to be responsible: t.ly/KKmGK. Collab with JLS lab @hhmijanelia.bsky.social

I’m excited to join this session organized by Assaf Zaritsky and Megan Driscoll for the "Beyond Pretty Pictures" subgroup. I’ll share our latest on Smart Microscopy and mitochondria.
#cellbio2024 @ascbiology.bsky.social

Sat. Dec. 14, 11:15am, Rm 30C

plan.core-apps.com/ascbembo2024/e…

Still on my oldies kick. Schwann proposed in his MDCCCXLVII monograph that muscle came from cell coalescence. "filaments are formed, which, in some situations, have an appearance like strings of pearls ... At a subsequent period, all trace of granules or division in the filament vanishes ..."

I’m reading some old mito classics, Margaret Reed and Walter Harmon Lewis with their camera lucida renderings. From various sources I thought the 1914 Science paper was ‘the one’, but on jstor there were no figures. Instead, it’s the 1915 AJA paper that’s a goldmine (Figs 6 & 10). Am I mistaken?

Here is a blue sky — an auspicious sign for the day I joined bluesky? Enjoying lovely Puerto Varas for the Chilean Cell Biology Meeting with my host Veronica Eisner. 💚