Sven T. Stripp
@stripplab.bsky.social
The Spectroscopy & Biocatalysis Group @ Uni Potsdam | DFG Heisenberg Fellow | Guest-Prof TU Berlin 2023/24 | Born at 338 ppm | Not a Strip Club
https://uni-potsdam.de/en/specbiocat | https://scholar.google.com/citations?user=ZtfyzRoAAAAJ&hl=en
https://uni-potsdam.de/en/specbiocat | https://scholar.google.com/citations?user=ZtfyzRoAAAAJ&hl=en
Let's grade this dissertation shall we
November 11, 2025 at 3:39 PM
Let's grade this dissertation shall we
I wonder if anyone ever got rejected from MDPI
November 11, 2025 at 2:28 PM
I wonder if anyone ever got rejected from MDPI
Amazing how easy math can be (with a teenager who always hated school) once it becomes a handy tool you actually need to get a job done... 🙏🏻
November 11, 2025 at 8:33 AM
Amazing how easy math can be (with a teenager who always hated school) once it becomes a handy tool you actually need to get a job done... 🙏🏻
Neubeck & Kirschning celebrate nickel (aka the second best transition metal) and its role in (geo-) chemical and biological evolution
doi.org/10.1016/j.ea...
doi.org/10.1016/j.ea...
November 10, 2025 at 6:50 AM
Neubeck & Kirschning celebrate nickel (aka the second best transition metal) and its role in (geo-) chemical and biological evolution
doi.org/10.1016/j.ea...
doi.org/10.1016/j.ea...
mechanistically, the Zundel ion may "safe-up" one proton to react with the hydride ligand at the H-cluster forming or splitting H2 as rapidly as the specific activities suggest...
Find all the details in our preprint‼️
doi.org/10.26434/che...
Find all the details in our preprint‼️
doi.org/10.26434/che...
November 6, 2025 at 12:40 PM
mechanistically, the Zundel ion may "safe-up" one proton to react with the hydride ligand at the H-cluster forming or splitting H2 as rapidly as the specific activities suggest...
Find all the details in our preprint‼️
doi.org/10.26434/che...
Find all the details in our preprint‼️
doi.org/10.26434/che...
Finally, QM calculations by Federica and Claudio reproduced the experimentally observed differences between Hox and HoxH - the previously mentioned "blue shift" - in the presence of a Zundel ion in the catalytic PT pathway.
November 6, 2025 at 12:40 PM
Finally, QM calculations by Federica and Claudio reproduced the experimentally observed differences between Hox and HoxH - the previously mentioned "blue shift" - in the presence of a Zundel ion in the catalytic PT pathway.
The XRD structure of the E279D variant revealed that protons shall easily travel toward a "conserved" water cluster (H4O2) while in wild-type enzyme, a gap between S319 and E279 blocks PT in the oxidized state.
We suggest that variant E279D can form a so-called "Zundel ion" (H5O2+) at low pH.
We suggest that variant E279D can form a so-called "Zundel ion" (H5O2+) at low pH.
November 6, 2025 at 12:40 PM
The XRD structure of the E279D variant revealed that protons shall easily travel toward a "conserved" water cluster (H4O2) while in wild-type enzyme, a gap between S319 and E279 blocks PT in the oxidized state.
We suggest that variant E279D can form a so-called "Zundel ion" (H5O2+) at low pH.
We suggest that variant E279D can form a so-called "Zundel ion" (H5O2+) at low pH.
So, what might cause the HoxH state? When revisiting our paper from 2018 we were puzzled to find that one variant of the catalytic PT pathway - E279D - seemed to adopt HoxH independent of dithionite. Luckily, this observation was reproducable. 😮💨
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 6, 2025 at 12:40 PM
So, what might cause the HoxH state? When revisiting our paper from 2018 we were puzzled to find that one variant of the catalytic PT pathway - E279D - seemed to adopt HoxH independent of dithionite. Luckily, this observation was reproducable. 😮💨
www.nature.com/articles/s41...
www.nature.com/articles/s41...
the same which is true for the IR profile. All variants adopted the HoxH state at low pH and in the presence of dithionite, just like wild-type enzyme. Can't say I was too happy when the results came in!
😑
😑
November 6, 2025 at 12:40 PM
the same which is true for the IR profile. All variants adopted the HoxH state at low pH and in the presence of dithionite, just like wild-type enzyme. Can't say I was too happy when the results came in!
😑
😑
Althought the variants clearly showed that water molecules in the PT pathway were missing, the H2 evolution activities were not signifiantly affected;
November 6, 2025 at 12:40 PM
Althought the variants clearly showed that water molecules in the PT pathway were missing, the H2 evolution activities were not signifiantly affected;
Disrupting the PT pathway at the backend had always been an idea - and frequent request from reviewers! - but we never sat down to produce the respective enzymes...
Now, seven years later, Jifu & his team went the extra mile and characterized three variants by biochemical methods and protein XRD.
Now, seven years later, Jifu & his team went the extra mile and characterized three variants by biochemical methods and protein XRD.
November 6, 2025 at 12:40 PM
Disrupting the PT pathway at the backend had always been an idea - and frequent request from reviewers! - but we never sat down to produce the respective enzymes...
Now, seven years later, Jifu & his team went the extra mile and characterized three variants by biochemical methods and protein XRD.
Now, seven years later, Jifu & his team went the extra mile and characterized three variants by biochemical methods and protein XRD.
The HoxH intermediate is important because a little earlier we had discovered that [FeFe]-hydrogenase in the HoxH state would adopt the so-called "hydride state" once reacted with H2. Previously, this had only been seen in variants of the catalytic PT pathway...
www.nature.com/articles/nco...
www.nature.com/articles/nco...
November 6, 2025 at 12:40 PM
The HoxH intermediate is important because a little earlier we had discovered that [FeFe]-hydrogenase in the HoxH state would adopt the so-called "hydride state" once reacted with H2. Previously, this had only been seen in variants of the catalytic PT pathway...
www.nature.com/articles/nco...
www.nature.com/articles/nco...
In 2018, we suggested that the H-cluster intermediate HoxH - distinct from Hox by a small "blue shift" of all CO/CN IR bands - is formed upon protonation of cysteine ligand C499 at the [4Fe-4S] backend of the H-cluster. An aqueous PT pathway seemed to lead toward C499.
doi.org/10.1039/C7CP...
doi.org/10.1039/C7CP...
November 6, 2025 at 12:40 PM
In 2018, we suggested that the H-cluster intermediate HoxH - distinct from Hox by a small "blue shift" of all CO/CN IR bands - is formed upon protonation of cysteine ligand C499 at the [4Fe-4S] backend of the H-cluster. An aqueous PT pathway seemed to lead toward C499.
doi.org/10.1039/C7CP...
doi.org/10.1039/C7CP...
I am very excited to share our new preprint - "A Zundel Ion in the Catalytic Proton Transfer Pathway of [FeFe]-Hydrogenase" - for which we revisited an older theory of ours, and came to surprising conclusions... 🧵
doi.org/10.26434/che...
doi.org/10.26434/che...
November 6, 2025 at 12:40 PM
I am very excited to share our new preprint - "A Zundel Ion in the Catalytic Proton Transfer Pathway of [FeFe]-Hydrogenase" - for which we revisited an older theory of ours, and came to surprising conclusions... 🧵
doi.org/10.26434/che...
doi.org/10.26434/che...
The authors mention that their results are in excellent agreement with this early 2024 preprint by Carr, Vincent, and Ash. Hopefully it will be published "properly" somewhere soon, I nearly forgot these data exist! 🫣
doi.org/10.26434/che...
doi.org/10.26434/che...
November 5, 2025 at 10:25 AM
The authors mention that their results are in excellent agreement with this early 2024 preprint by Carr, Vincent, and Ash. Hopefully it will be published "properly" somewhere soon, I nearly forgot these data exist! 🫣
doi.org/10.26434/che...
doi.org/10.26434/che...
Bielefeld, Marburg und Konstanz fühlen sich definitiv ähnlich an
November 4, 2025 at 8:18 PM
Bielefeld, Marburg und Konstanz fühlen sich definitiv ähnlich an
I've been using @chemrxiv.org since 2019. While waiting for a new submission to be approved, I did the math... More than 4.500 views per preprint (on average), not too bad!
November 4, 2025 at 10:09 AM
I've been using @chemrxiv.org since 2019. While waiting for a new submission to be approved, I did the math... More than 4.500 views per preprint (on average), not too bad!
When the proofs come in 🧪
November 3, 2025 at 7:21 PM
When the proofs come in 🧪
The 15yo is 16 years old today so we're having risotto
November 2, 2025 at 2:48 PM
The 15yo is 16 years old today so we're having risotto
Emotional concert by Henry "Shlohmo" Laufer tonight at Passionskirche 🥀🥀🥀
October 31, 2025 at 11:05 PM
Emotional concert by Henry "Shlohmo" Laufer tonight at Passionskirche 🥀🥀🥀
Inspired by dinuclear copper centers found in various metalloenzymes Tezcan et al. report the de novo design of a Cu-Cu paddlewheel coordination motif with a surprisingly negative reduction potential of −85 mV (via @jacs.acspublications.org).
doi.org/10.1021/jacs...
doi.org/10.1021/jacs...
October 31, 2025 at 1:54 PM
Inspired by dinuclear copper centers found in various metalloenzymes Tezcan et al. report the de novo design of a Cu-Cu paddlewheel coordination motif with a surprisingly negative reduction potential of −85 mV (via @jacs.acspublications.org).
doi.org/10.1021/jacs...
doi.org/10.1021/jacs...
Page 105, we're talking
October 31, 2025 at 9:59 AM
Page 105, we're talking
OK ANOTHER GRANT SUBMITTED 🚀
October 29, 2025 at 1:19 PM
OK ANOTHER GRANT SUBMITTED 🚀