Stefano Donati
@stefanodonati.bsky.social
Understanding and engineering microbial metabolism @ DTU Biosustain.
Just trying this BlueSky thing out... (@BioSteDonati on Twitter)
https://www.biosustain.dtu.dk/research/research-areas/sustainable-chemicals/c1-systems-biology
Just trying this BlueSky thing out... (@BioSteDonati on Twitter)
https://www.biosustain.dtu.dk/research/research-areas/sustainable-chemicals/c1-systems-biology
4/ 🙌 Big thanks to lead author Catarina Rocha and to the rest of the team Sergi Pujol Pinto, Sheila Ingemann Jensen, Lars Keld Nielsen
August 12, 2025 at 9:50 AM
4/ 🙌 Big thanks to lead author Catarina Rocha and to the rest of the team Sergi Pujol Pinto, Sheila Ingemann Jensen, Lars Keld Nielsen
3/ Biological insight:
• A comparative analysis of E. coli, P. putida, C. necator, B. subtilis, P. thermoglucosidasius
• PCA reveals strain- and condition-specific metabolic fingerprints
• P. thermoglucosidasius shows a lower adenylate energy charge than mesophiles
• A comparative analysis of E. coli, P. putida, C. necator, B. subtilis, P. thermoglucosidasius
• PCA reveals strain- and condition-specific metabolic fingerprints
• P. thermoglucosidasius shows a lower adenylate energy charge than mesophiles
August 12, 2025 at 9:50 AM
3/ Biological insight:
• A comparative analysis of E. coli, P. putida, C. necator, B. subtilis, P. thermoglucosidasius
• PCA reveals strain- and condition-specific metabolic fingerprints
• P. thermoglucosidasius shows a lower adenylate energy charge than mesophiles
• A comparative analysis of E. coli, P. putida, C. necator, B. subtilis, P. thermoglucosidasius
• PCA reveals strain- and condition-specific metabolic fingerprints
• P. thermoglucosidasius shows a lower adenylate energy charge than mesophiles
2/ Highlights:
• Cost-effective 13C-labeled internal standard production
• 96 metabolites quantified in <16 minutes
• Applicable across Gram-positive and Gram-negative species
• Captures inter- and intra-species metabolic phenotypes
• Cost-effective 13C-labeled internal standard production
• 96 metabolites quantified in <16 minutes
• Applicable across Gram-positive and Gram-negative species
• Captures inter- and intra-species metabolic phenotypes
August 12, 2025 at 9:50 AM
2/ Highlights:
• Cost-effective 13C-labeled internal standard production
• 96 metabolites quantified in <16 minutes
• Applicable across Gram-positive and Gram-negative species
• Captures inter- and intra-species metabolic phenotypes
• Cost-effective 13C-labeled internal standard production
• 96 metabolites quantified in <16 minutes
• Applicable across Gram-positive and Gram-negative species
• Captures inter- and intra-species metabolic phenotypes
P.S.: @angelgm.bsky.social you were right!
November 19, 2024 at 10:36 AM
P.S.: @angelgm.bsky.social you were right!
About me, I work at DTU Biosustain applying systems and synthetic biology approaches to microbial metabolism, focusing on assimilation of C1 compounds
www.biosustain.dtu.dk/research/res...
www.biosustain.dtu.dk/research/res...
C1 Systems Biology
In this group we study the metabolism of microbes capable of growth on CO2 and derivative molecules (C1 substrates). Our objective is to understand and engineer microorganisms suitable for sustainable...
www.biosustain.dtu.dk
November 19, 2024 at 10:36 AM
About me, I work at DTU Biosustain applying systems and synthetic biology approaches to microbial metabolism, focusing on assimilation of C1 compounds
www.biosustain.dtu.dk/research/res...
www.biosustain.dtu.dk/research/res...
A nice collaborative effort with @enricoorsi (first of many!) born after many long conversations. I am also grateful to @pabnik.bsky.social and Lars Nielsen for their feedback and mentorship.
October 21, 2023 at 11:34 AM
A nice collaborative effort with @enricoorsi (first of many!) born after many long conversations. I am also grateful to @pabnik.bsky.social and Lars Nielsen for their feedback and mentorship.