Siyuan (Steven) Wang
@sstevenwang.bsky.social
Image-based spatial multi-omics. Associate Professor at Yale. NIH Director's New Innovator Awardee. MIT Technology Review 35 Innovators Under 35.
Check out our new RAEFISH spatial transcriptomics technology published in Cell! This work represents the first time that transcripts from more than 20,000 genes were directly imaged in situ with any technology, and the first time numerous gRNAs were directly probed in an image-based CRISPR screen.
Now online! Sequencing-free whole-genome spatial transcriptomics at single-molecule resolution
Sequencing-free whole-genome spatial transcriptomics at single-molecule resolution
RAEFISH delivers whole-transcriptome (>20,000 genes) imaging at single-molecule resolution in cells and tissues and enables direct gRNA detection for high-content, image-based CRISPR screens.
dlvr.it
October 8, 2025 at 7:24 AM
Check out our new RAEFISH spatial transcriptomics technology published in Cell! This work represents the first time that transcripts from more than 20,000 genes were directly imaged in situ with any technology, and the first time numerous gRNAs were directly probed in an image-based CRISPR screen.
Reposted by Siyuan (Steven) Wang
New highlight video! 🧪🧬💻
RAEFISH from Steven Wang at Yale unlocks whole-transcriptome imaging at single-molecule resolution. See 20,000+ RNAs light up in cells and tissues. Plus get direct gRNA detection in high-content CRISPR screens. Spatial biology just leveled up
www.youtube.com/watch?v=P2j6...
RAEFISH from Steven Wang at Yale unlocks whole-transcriptome imaging at single-molecule resolution. See 20,000+ RNAs light up in cells and tissues. Plus get direct gRNA detection in high-content CRISPR screens. Spatial biology just leveled up
www.youtube.com/watch?v=P2j6...
Sequencing-free whole-genome spatial transcriptomics at single-molecule resolution
YouTube video by GenomeTDCC
www.youtube.com
October 1, 2025 at 5:17 PM
New highlight video! 🧪🧬💻
RAEFISH from Steven Wang at Yale unlocks whole-transcriptome imaging at single-molecule resolution. See 20,000+ RNAs light up in cells and tissues. Plus get direct gRNA detection in high-content CRISPR screens. Spatial biology just leveled up
www.youtube.com/watch?v=P2j6...
RAEFISH from Steven Wang at Yale unlocks whole-transcriptome imaging at single-molecule resolution. See 20,000+ RNAs light up in cells and tissues. Plus get direct gRNA detection in high-content CRISPR screens. Spatial biology just leveled up
www.youtube.com/watch?v=P2j6...
Reposted by Siyuan (Steven) Wang
🎉PUBLISHED @natgenet.nature.com
📰Tracing the evolution of single-cell 3D genomes in Kras-driven #cancers.
By Miao Liu, @muzumdarlab.bsky.social, @sstevenwang.bsky.social and colleagues.
⬇️
www.nature.com/articles/s41...
📰Tracing the evolution of single-cell 3D genomes in Kras-driven #cancers.
By Miao Liu, @muzumdarlab.bsky.social, @sstevenwang.bsky.social and colleagues.
⬇️
www.nature.com/articles/s41...
Tracing the evolution of single-cell 3D genomes in Kras-driven cancers - Nature Genetics
This study uses chromatin tracing to identify alterations in single-cell 3D genome conformation during the progression of Kras-driven mouse lung adenocarcinoma and pancreatic ductal adenocarcinoma, an...
www.nature.com
August 27, 2025 at 1:33 PM
🎉PUBLISHED @natgenet.nature.com
📰Tracing the evolution of single-cell 3D genomes in Kras-driven #cancers.
By Miao Liu, @muzumdarlab.bsky.social, @sstevenwang.bsky.social and colleagues.
⬇️
www.nature.com/articles/s41...
📰Tracing the evolution of single-cell 3D genomes in Kras-driven #cancers.
By Miao Liu, @muzumdarlab.bsky.social, @sstevenwang.bsky.social and colleagues.
⬇️
www.nature.com/articles/s41...
Reposted by Siyuan (Steven) Wang
New @natgenet.nature.com study from @muzumdarlab.bsky.social + @sstevenwang.bsky.social
maps 3D genomes in KRAS-driven lung & pancreatic #cancer, revealing stage-specific changes + potential biomarkers.
➡️ yalecancercenter.org/news-article...
maps 3D genomes in KRAS-driven lung & pancreatic #cancer, revealing stage-specific changes + potential biomarkers.
➡️ yalecancercenter.org/news-article...
Cancer's Progress Detailed by 3D Genomic Maps
Using new technology, Yale researchers examine cancer progression in single cells to visualize and understand its evolution from pre-cancerous to advanced
yalecancercenter.org
August 19, 2025 at 6:41 PM
New @natgenet.nature.com study from @muzumdarlab.bsky.social + @sstevenwang.bsky.social
maps 3D genomes in KRAS-driven lung & pancreatic #cancer, revealing stage-specific changes + potential biomarkers.
➡️ yalecancercenter.org/news-article...
maps 3D genomes in KRAS-driven lung & pancreatic #cancer, revealing stage-specific changes + potential biomarkers.
➡️ yalecancercenter.org/news-article...
Glad to share our latest publication in Nature Genetics: Tracing the evolution of single-cell 3D genomes in Kras-driven cancers. In this work, we generated the first single-cell 3D genome atlases of any cancer using any technique. 1/12
August 19, 2025 at 4:53 PM
Glad to share our latest publication in Nature Genetics: Tracing the evolution of single-cell 3D genomes in Kras-driven cancers. In this work, we generated the first single-cell 3D genome atlases of any cancer using any technique. 1/12
Check out this new video highlighting our lab :-) Thank you @yaleschoolofmed.bsky.social Video Team for the production! m.youtube.com/watch?v=p-hE...
Genome Architectures & Spatial Transcriptomics - The Siyuan Wang Lab at Yale School of Medicine
YouTube video by Yale Medicine
m.youtube.com
August 9, 2025 at 1:58 AM
Check out this new video highlighting our lab :-) Thank you @yaleschoolofmed.bsky.social Video Team for the production! m.youtube.com/watch?v=p-hE...
Reposted by Siyuan (Steven) Wang
How does 6ft of #DNA fit inside a cell only 10 microns wide?
@sstevenwang.bsky.social provides a brief history of contemporary 3D #genomics technologies. Learn how spatial organization of DNA plays a vital role in gene regulation, development, and disease. 🧬🖥️🧪 #STS
www.youtube.com/watch?v=gXCO...
@sstevenwang.bsky.social provides a brief history of contemporary 3D #genomics technologies. Learn how spatial organization of DNA plays a vital role in gene regulation, development, and disease. 🧬🖥️🧪 #STS
www.youtube.com/watch?v=gXCO...
A Brief History of Contemporary 3D Genomics Technologies
YouTube video by GenomeTDCC
www.youtube.com
July 30, 2025 at 3:53 PM
How does 6ft of #DNA fit inside a cell only 10 microns wide?
@sstevenwang.bsky.social provides a brief history of contemporary 3D #genomics technologies. Learn how spatial organization of DNA plays a vital role in gene regulation, development, and disease. 🧬🖥️🧪 #STS
www.youtube.com/watch?v=gXCO...
@sstevenwang.bsky.social provides a brief history of contemporary 3D #genomics technologies. Learn how spatial organization of DNA plays a vital role in gene regulation, development, and disease. 🧬🖥️🧪 #STS
www.youtube.com/watch?v=gXCO...
Reposted by Siyuan (Steven) Wang
Introducing Epi-PHR, the first non-disruptive, high-genomic-resolution, image-based epigenetic profiling method. Allelic Epi-PHR generates the first single-cell phased examination of histone mark deposition and 3D genome architecture at an imprinted locus by any technique. 1/8
June 26, 2025 at 4:29 AM
Introducing Epi-PHR, the first non-disruptive, high-genomic-resolution, image-based epigenetic profiling method. Allelic Epi-PHR generates the first single-cell phased examination of histone mark deposition and 3D genome architecture at an imprinted locus by any technique. 1/8
Introducing Epi-PHR, the first non-disruptive, high-genomic-resolution, image-based epigenetic profiling method. Allelic Epi-PHR generates the first single-cell phased examination of histone mark deposition and 3D genome architecture at an imprinted locus by any technique. 1/8
June 26, 2025 at 4:29 AM
Introducing Epi-PHR, the first non-disruptive, high-genomic-resolution, image-based epigenetic profiling method. Allelic Epi-PHR generates the first single-cell phased examination of histone mark deposition and 3D genome architecture at an imprinted locus by any technique. 1/8