SinghLab@SNIoE
@singhlab.bsky.social
Regenerative & Stress Biology Lab, Shiv Nadar University, Delhi-NCR, India
Regenerative Plasticity | Zebrafish | In vivo Fluorescent Imaging | Liver Regeneration | Starvation Response | Alcoholic Liver Disease
Regenerative Plasticity | Zebrafish | In vivo Fluorescent Imaging | Liver Regeneration | Starvation Response | Alcoholic Liver Disease
As part of the Faculty Development Program at Shiv Nadar University , received these 10 commandments for faculty. This is the best advice I have seen, and certainly hits the nail on the head. Especially about focusing and getting the first study out early on.
July 31, 2025 at 10:59 AM
As part of the Faculty Development Program at Shiv Nadar University , received these 10 commandments for faculty. This is the best advice I have seen, and certainly hits the nail on the head. Especially about focusing and getting the first study out early on.
Surprisingly, de novo hepatocytes emerged even when spared ones were present—a key deviation from the idea that cholangiocytes only activate after massive hepatocyte loss.
We confirmed this with a 17% partial hepatectomy model. ✂️🐟
(4/8)
We confirmed this with a 17% partial hepatectomy model. ✂️🐟
(4/8)
June 6, 2025 at 11:39 AM
Surprisingly, de novo hepatocytes emerged even when spared ones were present—a key deviation from the idea that cholangiocytes only activate after massive hepatocyte loss.
We confirmed this with a 17% partial hepatectomy model. ✂️🐟
(4/8)
We confirmed this with a 17% partial hepatectomy model. ✂️🐟
(4/8)
Enter CellCousin: a new zebrafish tool we designed to trace liver regeneration.
🟢 Spared hepatocytes
🔴 Ablated hepatocytes
🔵 De novo hepatocytes (not hepatocytes before injury)
This lets us precisely map where new hepatocytes come from after injury.
(3/8)
🟢 Spared hepatocytes
🔴 Ablated hepatocytes
🔵 De novo hepatocytes (not hepatocytes before injury)
This lets us precisely map where new hepatocytes come from after injury.
(3/8)
June 6, 2025 at 11:39 AM
Enter CellCousin: a new zebrafish tool we designed to trace liver regeneration.
🟢 Spared hepatocytes
🔴 Ablated hepatocytes
🔵 De novo hepatocytes (not hepatocytes before injury)
This lets us precisely map where new hepatocytes come from after injury.
(3/8)
🟢 Spared hepatocytes
🔴 Ablated hepatocytes
🔵 De novo hepatocytes (not hepatocytes before injury)
This lets us precisely map where new hepatocytes come from after injury.
(3/8)
How does DDCreER work?
We fused CreER to a destabilizing domain (DD) that is degraded unless stabilized by TMP.
💊 TMP = protein stabilization
🟣 4-OHT = nuclear translocation
Only when both are present does recombination occur—ensuring tight temporal control.
We fused CreER to a destabilizing domain (DD) that is degraded unless stabilized by TMP.
💊 TMP = protein stabilization
🟣 4-OHT = nuclear translocation
Only when both are present does recombination occur—ensuring tight temporal control.
May 28, 2025 at 3:18 PM
How does DDCreER work?
We fused CreER to a destabilizing domain (DD) that is degraded unless stabilized by TMP.
💊 TMP = protein stabilization
🟣 4-OHT = nuclear translocation
Only when both are present does recombination occur—ensuring tight temporal control.
We fused CreER to a destabilizing domain (DD) that is degraded unless stabilized by TMP.
💊 TMP = protein stabilization
🟣 4-OHT = nuclear translocation
Only when both are present does recombination occur—ensuring tight temporal control.
What is CellCousin?
It uses stochastic recombination and NTR-based ablation to remove only a subset of hepatocytes.
🟢 Spared cells
🔵 New (De novo) hepatocytes
This allows direct comparison of lineage contributions during liver repair.
It uses stochastic recombination and NTR-based ablation to remove only a subset of hepatocytes.
🟢 Spared cells
🔵 New (De novo) hepatocytes
This allows direct comparison of lineage contributions during liver repair.
May 28, 2025 at 3:18 PM
What is CellCousin?
It uses stochastic recombination and NTR-based ablation to remove only a subset of hepatocytes.
🟢 Spared cells
🔵 New (De novo) hepatocytes
This allows direct comparison of lineage contributions during liver repair.
It uses stochastic recombination and NTR-based ablation to remove only a subset of hepatocytes.
🟢 Spared cells
🔵 New (De novo) hepatocytes
This allows direct comparison of lineage contributions during liver repair.
Stunning 3D fluorescent images of the human #tooth and the first cellular/molecular map of tooth #decay! Hoang Thai Ha from Nicolas Baeyens Lab nails it with tissue clearing + imaging.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
May 16, 2025 at 6:16 PM
Stunning 3D fluorescent images of the human #tooth and the first cellular/molecular map of tooth #decay! Hoang Thai Ha from Nicolas Baeyens Lab nails it with tissue clearing + imaging.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Liver regeneration after killing part of the liver! In this image, the same liver from same #zebrafish is imaged before (left) and after (right) regeneration. The newly born liver cells are in blue.
Project led by @selifeski.bsky.social
Project led by @selifeski.bsky.social
May 14, 2025 at 6:53 PM
Liver regeneration after killing part of the liver! In this image, the same liver from same #zebrafish is imaged before (left) and after (right) regeneration. The newly born liver cells are in blue.
Project led by @selifeski.bsky.social
Project led by @selifeski.bsky.social
We celebrated a big milestone in our lab: our first #Undergrad intern presented her work at lab meeting! She helped develop two new #liver #injury models, and is on her way to becoming a fantastic scientist. Thanks to PhD student Garnik for mentoring her so well! 👏🧪 #TeamScience
May 3, 2025 at 6:33 AM
We celebrated a big milestone in our lab: our first #Undergrad intern presented her work at lab meeting! She helped develop two new #liver #injury models, and is on her way to becoming a fantastic scientist. Thanks to PhD student Garnik for mentoring her so well! 👏🧪 #TeamScience
Whole mount EdU staining of 5 day old #zebrafish.
April 12, 2025 at 5:34 PM
Whole mount EdU staining of 5 day old #zebrafish.
I'm excited to share that I will join School of Natural Sciences, Shiv Nadar University as an Associate Professor! Looking forward to working with amazing students and being part of a thriving research community.
April 8, 2025 at 7:26 AM
I'm excited to share that I will join School of Natural Sciences, Shiv Nadar University as an Associate Professor! Looking forward to working with amazing students and being part of a thriving research community.
Delighted to receive FOXO Transcription Factors edited by Dr. Wolfgang Link! FOXO genes are key in stress response & metabolism. Inés Garteizgogeascoa Suñer details a knock-in #zebrafish foxo1a reporter: pubmed.ncbi.nlm.nih.gov/39565585/ Check out the book for key advances!
March 3, 2025 at 9:16 AM
Delighted to receive FOXO Transcription Factors edited by Dr. Wolfgang Link! FOXO genes are key in stress response & metabolism. Inés Garteizgogeascoa Suñer details a knock-in #zebrafish foxo1a reporter: pubmed.ncbi.nlm.nih.gov/39565585/ Check out the book for key advances!
We only do for liver, where cryosections without any detergent work great with Nile Red (yellow), phalloidin (red) and Hoechst. We can share protocol. Works for fish and mouse :)
February 14, 2025 at 6:19 PM
We only do for liver, where cryosections without any detergent work great with Nile Red (yellow), phalloidin (red) and Hoechst. We can share protocol. Works for fish and mouse :)
Had a great time meeting the students at Freedom Employability Academy (FEA:
feaindia.org ) today! Inspiring to see their enthusiasm and growth. Excited to support their journey towards meaningful careers! #Mentorship #Education
feaindia.org ) today! Inspiring to see their enthusiasm and growth. Excited to support their journey towards meaningful careers! #Mentorship #Education
February 12, 2025 at 3:08 PM
Had a great time meeting the students at Freedom Employability Academy (FEA:
feaindia.org ) today! Inspiring to see their enthusiasm and growth. Excited to support their journey towards meaningful careers! #Mentorship #Education
feaindia.org ) today! Inspiring to see their enthusiasm and growth. Excited to support their journey towards meaningful careers! #Mentorship #Education
To validate these findings, we added a partial #hepatectomy (PHx) model—removing 17% of liver mass. Again, de novo hepatocytes emerged, highlighting cholangiocyte plasticity as a key regenerative mechanism, even after moderate injuries. ✂️🩺 (6/12)
January 10, 2025 at 7:57 AM
To validate these findings, we added a partial #hepatectomy (PHx) model—removing 17% of liver mass. Again, de novo hepatocytes emerged, highlighting cholangiocyte plasticity as a key regenerative mechanism, even after moderate injuries. ✂️🩺 (6/12)
Surprisingly, we found that de novo hepatocytes emerged even when spared hepatocytes were present. This challenges the prevailing idea that cholangiocyte-driven regeneration only kicks in after severe hepatocyte loss. 🤔 (5/12)
January 10, 2025 at 7:57 AM
Surprisingly, we found that de novo hepatocytes emerged even when spared hepatocytes were present. This challenges the prevailing idea that cholangiocyte-driven regeneration only kicks in after severe hepatocyte loss. 🤔 (5/12)
Here's how CellCousin works:
Hepatocytes are stochastically labeled green (spared) or red (ablated).
Upon injury, any blue-labeled hepatocytes that emerge are de novo—they weren’t hepatocytes before.
This gives us a clear picture of regeneration dynamics. 🎨🐟 (4/12)
Hepatocytes are stochastically labeled green (spared) or red (ablated).
Upon injury, any blue-labeled hepatocytes that emerge are de novo—they weren’t hepatocytes before.
This gives us a clear picture of regeneration dynamics. 🎨🐟 (4/12)
January 10, 2025 at 7:57 AM
Here's how CellCousin works:
Hepatocytes are stochastically labeled green (spared) or red (ablated).
Upon injury, any blue-labeled hepatocytes that emerge are de novo—they weren’t hepatocytes before.
This gives us a clear picture of regeneration dynamics. 🎨🐟 (4/12)
Hepatocytes are stochastically labeled green (spared) or red (ablated).
Upon injury, any blue-labeled hepatocytes that emerge are de novo—they weren’t hepatocytes before.
This gives us a clear picture of regeneration dynamics. 🎨🐟 (4/12)
Thank you FNRS for supporting our research for two more years! We recieved funding to look into #starvation-induced #fattyliver in #zebrafish. All the credit goes to PhD students who worked hard to get the data for the grant!
December 10, 2024 at 1:46 PM
Thank you FNRS for supporting our research for two more years! We recieved funding to look into #starvation-induced #fattyliver in #zebrafish. All the credit goes to PhD students who worked hard to get the data for the grant!
Delighted with two PhD students, Elif and Gabriel, who proudly represented the lab at Belgian Society of Stem Cell Research (BeSSCR) annual meeting, with Elif winning the best talk award!!
December 3, 2024 at 7:58 AM
Delighted with two PhD students, Elif and Gabriel, who proudly represented the lab at Belgian Society of Stem Cell Research (BeSSCR) annual meeting, with Elif winning the best talk award!!
Thank you Gilbert Weidinger Lab, University Ulm, for hosting me for a seminar and exciting discussion! It was great to walk around the city of Ulm as well and visit the Christmas market.
November 28, 2024 at 7:46 PM
Thank you Gilbert Weidinger Lab, University Ulm, for hosting me for a seminar and exciting discussion! It was great to walk around the city of Ulm as well and visit the Christmas market.
Using comparative transcriptomics, we identified a lipid transporter SLC27A2/FATP2 to be responsible for lack of starvation-induced fatty liver in cavefish. The Hua Bai Lab (Iowa State Uni) found that inhibiting FATP2 in flies reduced fatty liver, showing FATP2's role from flies to fish. (4/5)
January 12, 2024 at 11:08 AM
Using comparative transcriptomics, we identified a lipid transporter SLC27A2/FATP2 to be responsible for lack of starvation-induced fatty liver in cavefish. The Hua Bai Lab (Iowa State Uni) found that inhibiting FATP2 in flies reduced fatty liver, showing FATP2's role from flies to fish. (4/5)
We have shown that zebrafish larvae develop fatty liver upon starvation. With @rohner.bsky.social Lab we asked if Mexican cavefish, an amazing model of starvation resistance, collect excess fat to survive long periods of starvation. To our surprise, they did not get fatty liver!!(3/5)
January 12, 2024 at 11:07 AM
We have shown that zebrafish larvae develop fatty liver upon starvation. With @rohner.bsky.social Lab we asked if Mexican cavefish, an amazing model of starvation resistance, collect excess fat to survive long periods of starvation. To our surprise, they did not get fatty liver!!(3/5)
Since the discovery of Dr Williams, starvation-induced #fattyliver has been observed in animals (flies, mouse, cat and minks). In humans, patients suffering from anorexia nervosa or bulimia can develop #fattyliver. Storage of fat in the liver is evolutionarily conserved (2/5)
January 12, 2024 at 11:06 AM
Since the discovery of Dr Williams, starvation-induced #fattyliver has been observed in animals (flies, mouse, cat and minks). In humans, patients suffering from anorexia nervosa or bulimia can develop #fattyliver. Storage of fat in the liver is evolutionarily conserved (2/5)
Fatty Liver is linked with a high fat diet and obesity. But 90 years ago, Dr Cicely Williams, found that malnourished babies develop fatty liver. Now, @rohner.bsky.social , Hua Bai and our lab found the gene responsible for starvation-induced fatty liver from flies to fish! A 🧵(1/5)
January 12, 2024 at 11:05 AM
Fatty Liver is linked with a high fat diet and obesity. But 90 years ago, Dr Cicely Williams, found that malnourished babies develop fatty liver. Now, @rohner.bsky.social , Hua Bai and our lab found the gene responsible for starvation-induced fatty liver from flies to fish! A 🧵(1/5)
Was a pleasure to meet with @simoesfilipa.bsky.social and listen about the role of macrophages in heart #regeneration in #zebrafish! Lots of interesting things to come from her group!!
November 24, 2023 at 3:12 PM
Was a pleasure to meet with @simoesfilipa.bsky.social and listen about the role of macrophages in heart #regeneration in #zebrafish! Lots of interesting things to come from her group!!