However, NASP cannot do it all. It is supported by (1) the INO80 complex which remodels chromatin to evict histones, (2) which NASP then stabilises and stores and (3) PARP1 and other factors help reincorporate them. (6/8)

Lose NASP and even resistant tumor cells become resistant to PARPi. (5/8)

What are the factors mediating this histone release? To answer this we performed genome-wide genetic screens and identified NASP, a histone chaperone, as essential for handling these evicted histones. (4/8)

The answer was puzzling: Histones got kicked off the chromatin upon PARPi exposure. These free histones must be stored & recycled, otherwise cancer cells struggle to survive. (3/8)