We experimentally show that two PGHs encoded by salt-loving archaeon Halogranum salarium B-1 do kill one of the predicted targets, salt-tolerant bacterium Halalkalibacterium halodurans. 5/6

We developed a structural homology-based pipeline to infer the putative bacterial targets of these proteins and found that most seem to target monoderm bacteria living in the same environment as the producer. 4/6

Here, we show that about 5% of archaea encode peptidoglycan hydrolases (PGHs) in their genomes, though most archaea do not use peptidoglycan. They are enriched on plasmids and display a high level of structural conservation with their bacterial counterpart, hinting at their use as weapons. 3/6