Bharath Kumar Raghuraman
@rbharathkumar91.bsky.social
Proteomics expert | Clinical proteomics | Phosphatase drug discovery at Anavo Therapeutics
Reposted by Bharath Kumar Raghuraman
Leading these (and many ongoing) projects has been demanding, exhilarating & rewarding.
They required highly coordinated collaborations among diverse experts within PTI, MIT, MGH and beyond.
1/2
They required highly coordinated collaborations among diverse experts within PTI, MIT, MGH and beyond.
1/2
May 30, 2025 at 1:24 PM
Leading these (and many ongoing) projects has been demanding, exhilarating & rewarding.
They required highly coordinated collaborations among diverse experts within PTI, MIT, MGH and beyond.
1/2
They required highly coordinated collaborations among diverse experts within PTI, MIT, MGH and beyond.
1/2
Reposted by Bharath Kumar Raghuraman
The corresponding author on this work @searleb.bsky.social has posted a tutorial diving in on the MAP-MS approach and how it improves MS1 dynamic range:
www.linkedin.com/pulse/how-ma...
www.linkedin.com/pulse/how-ma...
May 29, 2025 at 8:56 PM
The corresponding author on this work @searleb.bsky.social has posted a tutorial diving in on the MAP-MS approach and how it improves MS1 dynamic range:
www.linkedin.com/pulse/how-ma...
www.linkedin.com/pulse/how-ma...
Reposted by Bharath Kumar Raghuraman
Almost intentionally, looks like all of the new Thermo instrument pages are up (Astral Zoom, Excedion Pro, Optispray).
Overall vibes?
Excedion is the bigger upgrade, almost 2x faster and can now do ETD. Astral Zoom improvements are more incremental.
No change to instrument sensitivity specs.
Overall vibes?
Excedion is the bigger upgrade, almost 2x faster and can now do ETD. Astral Zoom improvements are more incremental.
No change to instrument sensitivity specs.
May 28, 2025 at 11:04 AM
Almost intentionally, looks like all of the new Thermo instrument pages are up (Astral Zoom, Excedion Pro, Optispray).
Overall vibes?
Excedion is the bigger upgrade, almost 2x faster and can now do ETD. Astral Zoom improvements are more incremental.
No change to instrument sensitivity specs.
Overall vibes?
Excedion is the bigger upgrade, almost 2x faster and can now do ETD. Astral Zoom improvements are more incremental.
No change to instrument sensitivity specs.
Reposted by Bharath Kumar Raghuraman
optTMT: A Shiny application to optimize any experimental design to minimize the TMT reporter ion interference zenodo.org/records/1...
---
#proteomics #prot-other
---
#proteomics #prot-other
February 18, 2025 at 8:40 AM
optTMT: A Shiny application to optimize any experimental design to minimize the TMT reporter ion interference zenodo.org/records/1...
---
#proteomics #prot-other
---
#proteomics #prot-other
Reposted by Bharath Kumar Raghuraman
Excited to share this new work led by postdoc Brendan Floyd, deep mapping of cell-surface protein interactomes of T and B cells reveals maverick mitochondrial proteins and immune modulatory complexes.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Mapping the nanoscale organization of the human cell surface proteome reveals new functional associations and surface antigen clusters
The cell surface is a dynamic interface that controls cell-cell communication and signal transduction relevant to organ development, homeostasis and repair, immune reactivity, and pathologies driven b...
www.biorxiv.org
February 18, 2025 at 7:27 PM
Excited to share this new work led by postdoc Brendan Floyd, deep mapping of cell-surface protein interactomes of T and B cells reveals maverick mitochondrial proteins and immune modulatory complexes.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Bharath Kumar Raghuraman
Looks like another fantastic proteomics data tool from the @kusterlab.bsky.social Thanks a lot, must read for sure.
🎉 We're happy to announce that our latest project was published in @naturecomms.bsky.social this week: PTMNavigator, a #bioinformatics web platform for in-depth analysis of post-translational modification (PTM) perturbation datasets.
📄 doi.org/10.1038/s414... (1/6)
📄 doi.org/10.1038/s414... (1/6)
PTMNavigator: interactive visualization of differentially regulated post-translational modifications in cellular signaling pathways - Nature Communications
Post-translational modifications are important regulators of cellular pathways, but our understanding of these processes is limited. Here, the authors present a web tool that integrates various databa...
doi.org
January 14, 2025 at 6:55 AM
Looks like another fantastic proteomics data tool from the @kusterlab.bsky.social Thanks a lot, must read for sure.
Reposted by Bharath Kumar Raghuraman
If you’re thinking of what meeting to go to this year then check out the #phosphatase meeting in Würzburg. Lots of great speaking slots and events for junior researchers. It’s always a fun meeting meetings.embo.org/event/25-pho...
Phosphatases: from basic research to translation
Phosphatases have been considered undruggable for decades. However, recent breakthroughs with the first small molecule phosphatase inhibitors for cancer immunotherapy now in clinical trials have resu…
meetings.embo.org
January 17, 2025 at 6:58 PM
If you’re thinking of what meeting to go to this year then check out the #phosphatase meeting in Würzburg. Lots of great speaking slots and events for junior researchers. It’s always a fun meeting meetings.embo.org/event/25-pho...
Reposted by Bharath Kumar Raghuraman
Not bad for some boring work of a bunch of nerds 🤓 😂
proteomicsnews.blogspot.com/2025/01/boring…
@proteomicsnews.bsky.social
proteomicsnews.blogspot.com/2025/01/boring…
@proteomicsnews.bsky.social
https://proteomicsnews.blogspot.com/2025/01/boring…
January 10, 2025 at 9:09 PM
Not bad for some boring work of a bunch of nerds 🤓 😂
proteomicsnews.blogspot.com/2025/01/boring…
@proteomicsnews.bsky.social
proteomicsnews.blogspot.com/2025/01/boring…
@proteomicsnews.bsky.social
Reposted by Bharath Kumar Raghuraman
Excited to share our work investigating how ligand residence time influences targeted protein degradation outcomes at scale, and how we can combine kinetic scout degrader approaches with mathematical modeling to accelerate hit finding and optimization!
onlinelibrary.wiley.com/doi/abs/10.1...
onlinelibrary.wiley.com/doi/abs/10.1...
A Kinetic Scout Approach Accelerates Targeted Protein Degrader Development
Bifunctional molecules such as targeted protein degraders induce proximity to promote gain-of-function pharmacology. These powerful approaches have gained broad traction across academia and the pharm....
onlinelibrary.wiley.com
November 27, 2024 at 10:29 PM
Excited to share our work investigating how ligand residence time influences targeted protein degradation outcomes at scale, and how we can combine kinetic scout degrader approaches with mathematical modeling to accelerate hit finding and optimization!
onlinelibrary.wiley.com/doi/abs/10.1...
onlinelibrary.wiley.com/doi/abs/10.1...
Reposted by Bharath Kumar Raghuraman
Enjoy the weekend with a discussion about proteomic technologies & the biology progress that they can enable.
They may unblock biology research.
www.youtube.com/watch?v=sGW4...
They may unblock biology research.
www.youtube.com/watch?v=sGW4...
Unveiling Proteins: The Future Of Single-Cell Proteomics With Professor Nikolai Slavov
YouTube video by ARK Invest
www.youtube.com
November 22, 2024 at 12:22 PM
Enjoy the weekend with a discussion about proteomic technologies & the biology progress that they can enable.
They may unblock biology research.
www.youtube.com/watch?v=sGW4...
They may unblock biology research.
www.youtube.com/watch?v=sGW4...
Reposted by Bharath Kumar Raghuraman
This paper should be cited in the response to every reviewer questions asking to validate MS results by WB..
November 21, 2024 at 1:10 PM
This paper should be cited in the response to every reviewer questions asking to validate MS results by WB..
Worth a read
Excellent thread about affinity vs MS for plasma proteomics. It is 100% worth a read
I spend a bit of time talking to folk with plasma cohorts about MS vs affinity approaches for discovery. Thought it was worth a thread.
Most love the marketing. 5,000 proteins? 10,000 proteins? OLink was used in UK Biobank? Wow, mass spec is sooooo last year. Except life is not that simple.
Most love the marketing. 5,000 proteins? 10,000 proteins? OLink was used in UK Biobank? Wow, mass spec is sooooo last year. Except life is not that simple.
November 21, 2024 at 1:33 PM
Worth a read
Reposted by Bharath Kumar Raghuraman
PXD036000 🚨
SHP099, an inhibitor of the protein-tyrosine phosphatase SHP2 (encoded by the PTPN11 gene) and trametinib (MEKi) in an NF1 MPNST patient.
🚨 New dataset alert! 🚨
SHP099, an inhibitor of the protein-tyrosine phosphatase SHP2 (encoded by the PTPN11 gene) and trametinib (MEKi) in an NF1 MPNST patient.
🚨 New dataset alert! 🚨
November 21, 2024 at 7:00 AM
PXD036000 🚨
SHP099, an inhibitor of the protein-tyrosine phosphatase SHP2 (encoded by the PTPN11 gene) and trametinib (MEKi) in an NF1 MPNST patient.
🚨 New dataset alert! 🚨
SHP099, an inhibitor of the protein-tyrosine phosphatase SHP2 (encoded by the PTPN11 gene) and trametinib (MEKi) in an NF1 MPNST patient.
🚨 New dataset alert! 🚨
Reposted by Bharath Kumar Raghuraman
We are! … or will be, we just had a hiccup with the xodus. Last year when we ditched spaces due to Musk, we went back to OG clubhouse. This time we (@ucdproteomics.bsky.social @leannewg.bsky.social) had to chat about it again, hence the delay. Expect announcements here and likely a Dec 4 POTRH.
November 19, 2024 at 11:16 AM
We are! … or will be, we just had a hiccup with the xodus. Last year when we ditched spaces due to Musk, we went back to OG clubhouse. This time we (@ucdproteomics.bsky.social @leannewg.bsky.social) had to chat about it again, hence the delay. Expect announcements here and likely a Dec 4 POTRH.
@benneely.com any plans for continuing POTRH here?
November 19, 2024 at 8:47 AM
@benneely.com any plans for continuing POTRH here?