Tino Pleiner
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pleinerlab.bsky.social
Tino Pleiner
@pleinerlab.bsky.social
110 followers 75 following 1 posts
Assistant Professor @Stanford_Molecular & Cellular Physiology; Membrane protein homeostasis; Protein quality control; Antibody engineering; Engineering cells with genetically encodable #nanobodies, #FirstGen; Pronouns: He/Him
https://www.pleinerlab.org/
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pleinerlab.bsky.social
Tino Pleiner @pleinerlab.bsky.social · Dec 18
My lab @Stanford studies membrane protein insertion, assembly and quality control at the human ER membrane. We develop tiny genetically encodable antibodies from alpacas, called nanobodies, as tools to acutely manipulate intracellular biology. Join us as a postdoc! Apply here: bit.ly/_Postdocs
Reposted by Tino Pleiner
laskerfdn.bsky.social
2025 Basic Award winner Dirk Görlich @mpsgoettingen.bsky.social advises scientists to overcome "headwinds against new ways of thinking" by putting ideas to the "strictest possible test" and finding satisfaction in the journey, not just the destination. #LaskerAwards
September 29, 2025 at 4:33 PM
Reposted by Tino Pleiner
denkenny.bsky.social
✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv

Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo

🔗 www.biorxiv.org/content/10.1...
October 8, 2025 at 3:59 PM
Reposted by Tino Pleiner
rhuttenhain.bsky.social
My lab @stanfordmedicine.bsky.social is recruiting! We are looking for a postdoc at the interface of quantitative proteomics and G protein-coupled receptor (GPCR) biology:
postdocs.stanford.edu/prospective/...
#TeamMassSpec #Proteomics #GPCR #Postdoc
Open Postdoctoral position, faculty mentor Ruth Huttenhain | Office of Postdoctoral Affairs
postdocs.stanford.edu
October 2, 2025 at 6:27 PM
Reposted by Tino Pleiner
samuelmaiwald.bsky.social
Excited to share our latest study on how K29/K48-branched #ubiquitin chains are forged by the #E3 ligase TRIP12, and how this suggests a consensus mechanism for chain formation by HECT E3s!

@natsmb.nature.com

1/7

www.nature.com/articles/s41...
TRIP12 structures reveal HECT E3 formation of K29 linkages and branched ubiquitin chains - Nature Structural & Molecular Biology
Using biochemistry, chemical biology, and cryo-EM, Maiwald et al. elucidate how TRIP12 forms K29 linkages and K29/K48-linked branched ubiquitin chains, revealing a mechanism for polyubiquitylation sha...
www.nature.com
May 26, 2025 at 9:37 AM
Reposted by Tino Pleiner
cahirokane.bsky.social
ER needs ATP for processes like protein quality control, but can't make its own. Here, how ATP is imported into the ER lumen and exchanged for ADP.
May 24, 2025 at 4:56 AM
Reposted by Tino Pleiner
carolynbertozzi.bskyverified.social
David Liu @harvard.edu beautifully articulates the criticality of basic science funding for developing revolutionary therapeutics like life-saving base editors 👏

youtu.be/8YhJM6zxYDw?...
Breakthrough Prize-Winning Biochemist on the Deadly Cost of Funding Cuts | Amanpour and Company
YouTube video by Amanpour and Company
youtu.be
May 24, 2025 at 1:13 AM
Reposted by Tino Pleiner
micharapelab.bsky.social
Amazing story from #MolecularTherapeutics faculty @robzonculab.bsky.social with lead author @aakritijain.bsky.social describing membrane protein degradation in the lysosome. Congratulations!!

www.science.org/doi/10.1126/...
Leucine aminopeptidase LyLAP enables lysosomal degradation of membrane proteins
Breakdown of every transmembrane protein trafficked to lysosomes requires proteolysis of their hydrophobic helical transmembrane domains. Combining lysosomal proteomics with functional genomic dataset...
www.science.org
March 27, 2025 at 9:36 PM
Reposted by Tino Pleiner
mratz.bsky.social
Excited to share our paper with Jakobs Lab @mpi-nat.bsky.social on the in situ structure of the prohibitin complex in human mitochondria! Although mitochondria contain >1000 proteins, it seems a single, 2-subunit complex occupies 1-3% of the crista membrane area 🤯

www.nature.com/articles/s41...
In situ architecture of the human prohibitin complex - Nature Cell Biology
Lange, Ratz, et al. investigate the number and distribution of human prohibitin complexes in the mitochondrial inner membrane, uncovering their bell-shaped structure and assembly of alternating PHB1 a...
www.nature.com
March 25, 2025 at 8:47 PM
Reposted by Tino Pleiner
carolynbertozzi.bskyverified.social
Exciting discovery by Svensson lab and collaborators @stanfordmedicine.bsky.social, computational discovery of 2500 new bioactive peptides, including 12-mer named BRP that reduces food intake leading to weight loss w/o nausea in mice!

www.nature.com/articles/s41...
Prohormone cleavage prediction uncovers a non-incretin anti-obesity peptide - Nature
Computational drug discovery is used to identify a 12-mer peptide derived from BRINP2 with potent anti-obesity effects that are independent of leptin, glucagon-like peptide 1 receptor and melanocortin...
www.nature.com
March 11, 2025 at 11:22 PM
Reposted by Tino Pleiner
sylviecallegari.bsky.social
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...

@wehi-research.bsky.social @komanderlab.bsky.social
March 13, 2025 at 7:20 PM
Reposted by Tino Pleiner
danieljdrucker.bsky.social
The 2025 Wolf Prize Laureate in Medicine, Professor Pamela J. Bjorkman “For pioneering innovative strategies to overcome viral defenses through novel antibody-focused approaches” @caltech.edu wolffund.org.il/pamela-j-bjo... @bjorkman-lab.bsky.social
Pamela J. Bjorkman
Wolf Prize Laureate in Medicine 2025
wolffund.org.il
March 10, 2025 at 7:38 PM
Reposted by Tino Pleiner
clausenlab.bsky.social
scary but fascinating - cells have a degradation pathway that hunts down C-terminal scars (amides!) on damaged proteins. just wow. chemical biology plus CRISPR at its best. big congrats to all authors!

www.nature.com/articles/s41...
C-terminal amides mark proteins for degradation via SCF–FBXO31 - Nature
SCF–FBXO31 scans proteins for C-terminal amidation and marks them for subsequent proteasomal degradation.
www.nature.com
January 29, 2025 at 5:57 PM
Reposted by Tino Pleiner
benlehner.bsky.social
Nice highlighting of @taylor-mighell.bsky.social's GPCR pharmacochaperone preprint by @dereklowe.bsky.social @science.org www.biorxiv.org/content/10.1...
January 19, 2025 at 2:05 PM
Reposted by Tino Pleiner
becklab.bsky.social
In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:
January 17, 2025 at 6:43 PM
Reposted by Tino Pleiner
mick-lab.bsky.social
Proud to share our work spear-headed by PhD student Tommy Sroka with major contributions by #xenopus expert Kerstin Feistel.

Our iAPEX #ProximityLabeling method for #MassSpec based subcellular #proteomics works by locally generating H2O2 using a D-amino acid oxidase that activates APEX2 in situ.
iAPEX: Improved APEX-based proximity labeling for subcellular proteomics using an enzymatic reaction cascade
Ascorbate peroxidase (APEX) is a versatile labeling enzyme used for live-cell proteomics at high spatial and temporal resolution. However, toxicity of its substrate hydrogen peroxide and background la...
www.biorxiv.org
January 11, 2025 at 7:51 PM
Reposted by Tino Pleiner
cellsandprots.bsky.social
Protein quality control is a tug-of-war between degradation machinery and chaperones that promote folding. Thrilled to see our latest manuscript is now published!
👇 Follow 🧵 for quick summary
journals.biologists.com/jcs/article-...
January 10, 2025 at 5:10 PM
Reposted by Tino Pleiner
mikelangelipid.bsky.social
I am excited to report the preprint of my postdoc work in the @olzmannlab.bsky.social! We have discovered the first lipid droplet quality control pathway with broad implications for lipid physiology and diseases associated with LD accumulation and oxidative stress! #lipidtime

shorturl.at/2HqFd
January 8, 2025 at 1:46 PM
Reposted by Tino Pleiner
willowcoyote.bsky.social
Check out our work, where we discover how receptor GPCR senses pH! We solved a longstanding question by developing foundational GPCR DMS tech dev + structural bio! Led by brilliant PhD student @matthewkhoward.bsky.social and Nick Hoppe w/ @amanglik.bsky.social!

www.cell.com/cell/fulltex...
January 6, 2025 at 11:47 PM
Reposted by Tino Pleiner
biancaschrul.bsky.social
Interested in #organelle biogenesis, subcellular #membrane #protein targeting or #LipidMetabolism? We are hiring! #PhD student or #postdoc.
We combine #Biochemistry & #CellBiology with Computational Modeling & Experimental Physics / Physical Chemistry approaches.
#research #academicsky #AcademicJob
November 26, 2024 at 2:51 PM
Reposted by Tino Pleiner
cellarchlab.com
I’ll have more to say about this later, but please have a look, and I hope the #TeamTomo community finds good uses for those 1829 Chlamy tomograms 🧪🧶🧬🌾🔬
December 29, 2024 at 4:17 PM
Reposted by Tino Pleiner
wallaceucsf.bsky.social
Mitochondria consist of networks of cylindrical tubes, right? Not necessarily! - in our new preprint, @gavsturm.bsky.social investigates how mitochondria transiently adopt a beads-on-a-string morphology

www.biorxiv.org/content/10.1...
December 23, 2024 at 5:49 PM
pleinerlab.bsky.social
My lab @Stanford studies membrane protein insertion, assembly and quality control at the human ER membrane. We develop tiny genetically encodable antibodies from alpacas, called nanobodies, as tools to acutely manipulate intracellular biology. Join us as a postdoc! Apply here: bit.ly/_Postdocs
December 18, 2024 at 12:37 AM
Reposted by Tino Pleiner
mpi-nat.bsky.social
Görlich lab develops secondary #nanobodies! They outperform conventional secondary antibodies and can replace these and their controversial production in animals: Nanobodies can be produced in large scale by bacteria. @JCellBiol http://www.mpibpc.mpg.de/15856308/pr_1726
November 25, 2024 at 6:41 AM