Nice afternoon spent learning about the clinical status of tick borne diseases here in Denmark. Still lot convinced by the Pfizer vaccine though #borrelia #tbe #tickborneencephalitusvirus

Second conference of 2024! This time my side-pathogen #borrelia and will be presenting my work on using blood-brain barrier #organoids to investigate #neuroborreliosis It’s all about the barrier!! Onwards to Fyn and #Nordtick

Happy #FluorescenceFriday Normally, I'm all about the endothelium, but this time it's some lovely tight-junctions from an epithelial monolayer. There are multiple barriers in the human brain and now we are focusing on the blood-CSF barrier #malaria #neuroscience

In 2021 I was awarded funding by the Danish 3Rs/Landbrugsstyrelsen to work on BBB organoids. We've shown they can model cerebral malaria and tissue tropism in Lyme disease. You can read more about it in more detail here: bit.ly/3XbVnUg and here bit.ly/3167j5q
#borrelia #neuroscience #malaria

Fantastic afternoon of all things 3D cell culture/organoids/spheroids. So many interesting talks covering a range of tissues and organs, from my personal favourite the BBB, to the retina, intestinal organoids, tonsil/thymus organoids, liver and full thickness skin models 🤯 #malaria #neuroscience

By comparing multiple isolates from Lyme neuro, non-Lyme neuro and N America, we observed only those from Lyme neuro patients crossing the barrier

…and we don’t just use them for cerebral malaria. We also use them to investigate Lyme neuroborreliosis more commonly found here in Europe.

In doing so, this leads to swelling and BBB dysfunction

By utilizing ICAM-1, a subset can enter endothelial cells and cross the BBB

So what do we do in my lab? 🤔 We use BBB-organoids to study how RBCs infected with malaria parasites interact with ICAM-1….