Osvaldo Burastero
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osvalb.bsky.social
Osvaldo Burastero
@osvalb.bsky.social
850 followers 950 following 25 posts
Biophysics enthusiast. Currently focused on data analysis: https://spc.embl-hamburg.de
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osvalb.bsky.social
Do you want to analyze mass photometry (MP) count data with Python scripts? Try pyPhotoMol, a package to process, fit, and visualize the MP data 👨‍💻

github.com/osvalB/pypho...

pyPhotoMol is derived from PhotoMol, our web tool for MP:

spc.embl-hamburg.de/app/photoMol

#massphotometry #Python #code
Simulated mass photometry results showing count distributions at a constant concentration of A (150 kDa) and seven different concentrations of B (30 kDa). B free is under the limit of detection (40 kDa). A and B interact with an equilibrium dissociation constant (Kd) of 1 nM. Screenshot of the pyPhotoMol package repository (https://github.com/osvalB/pyphotomol)
August 13, 2025 at 9:45 AM
osvalb.bsky.social
Interested in antimicrobial resistance and drug discovery? Attend the talk of my colleague and partner Tania Szal 😊
Monday 17th at 8:30. Platform: protein-small molecule interactions I
#BPS2025
February 14, 2025 at 2:52 PM
osvalb.bsky.social
Looking forward to making new connections at #BPS2025👨 Meet me at poster B38 on Tuesday 18 Feb 13:45. Session "protein stability folding and chaperones"
espc poster details BPS conference banner
February 14, 2025 at 2:41 PM
osvalb.bsky.social
Just finished a science poster (in Figma) and wanted to share the template I used! 🎨 Hope it's useful: github.com/osvalB/figma...
Poster template done in Figma
February 11, 2025 at 11:51 AM
osvalb.bsky.social
The estimated Kd (micromolar) is 1.3, and we also provide the asymmetric confidence interval: [0.5 - 3.1] (link.springer.com/article/10.1...)

The reported Kd by the authors was 1.2.

6. Extra: I scaled the plot to start around 0.
Points: measured MST signal. Line: Fitted curved based on the estimated Kd of 1.3 micromolar.
January 31, 2025 at 10:24 AM
osvalb.bsky.social
4. Go to the '2. Fitting' section. Inside the 'Advanced settings', set the Kd max value to 5 micromolar (to help the fitting algorithm 😄😬 ).

5. Press on 'Run fitting' and voila!
Screenshot of ThermoAffinity showing that the Kd upper bound (for the fitting) is 5 micromolar.
January 31, 2025 at 10:24 AM
osvalb.bsky.social
2. Import the file into our tool ThermoAffinity (spc.embl-hamburg.de/app/thermoAf...)

3. Set the units to 'Nanomolar' and the hot region between 14 - 20 seconds (based on what the authors reported)
Screenshot of ThermoAffinity showing that the selected units are 'Nanomolar', and that the hot region interval goes from 14 to 20 seconds. Microscale thermophoresis traces of the entry https://mbdb-data.org/mst/5s81t-w6s49
January 31, 2025 at 10:24 AM
osvalb.bsky.social
Are you working with circular dichroism (CD)? Check out the latest update to our ChiraKit tool, now with 10 tutorials to get you started! 🚀 Try it here: spc.embl-hamburg.de/app/chirakit
An abstract representation of what happens during a circular dichroism measurement. Left- and right-circularly polarized light passes through the sample. One of the components is more absorbed.
January 29, 2025 at 4:00 PM
osvalb.bsky.social
Just out! 🥳🥳 Great work done at the @spcembl.bsky.social to understand clathrin-mediated endocytosis. We investigated how clathrin and adaptor proteins interact in vitro and in vivo. Kudos to the 1st author @lucasdefelipe.bsky.social!

www.nature.com/articles/s41...

www.embl.org/news/science...
November 18, 2024 at 9:30 AM