Always great to connect and share science with colleagues from the Genome Biology Program at @crg.eu
🦠🔬🏖️

Big thanks to the @crg.eu community and the Novoa lab memebers for all the support and discussions.

Please read us and share your comments! :)

m6ABasecaller can predict m6A modifications at SINGLE MOLECULE resolution and we use it to ask exciting biological questions: we explored the cross-talk between m6A sites within the same read, the interaction between m6A and polyA tail lengths, the m6A differences between isoforms and much more!

You can use the m6ABasecaller on a stand-alone sample, without the need for a WT/KO comparison to identify your sites to call m6A modifications completely de novo in different species (e.g. human, mouse, zebrafish…)

But what’s the big deal with modification basecalling?

Yes, it was developed with RNA002 chemistry, so if you have RNA002 sequencing data you can use m6ABasecaller to identify m6A in individual reads directly during the basecalling process. If you only have RNA004 data, there’s also promising results about it so stay tuned for updates from Novoa lab ;)

Leveraging the tool NanoRMS2 (github.com/novoalab/nan...) we generated labels to train the first modification-aware basecaller for m6A, which we called m6ABasecaller (github.com/novoalab/m6A...).

This was a great collaborative effort co-lead by @soniacruciani.bsky.social, Anna Delgado-Tejedor and Leszek Pryszcz with the help of Rebeca Medina and Laia Llovera and under the supervision of @evamarianovoa.bsky.social