Makaía Papasergi-Scott
@mpapasergiscott.bsky.social
Structural Biology. Cell Biology. G protein signaling. GPCRs. Protein structure & biochemistry. Membrane proteins. CryoEM/CryoET. Microscopes. Asst Prof at University of Rochester.
Pinned
Time-resolved cryo-EM of G-protein activation by a GPCR - Nature
Time-resolved cryo-EM is used to capture structural transitions during G-protein activation stimulated by a G-protein-coupled receptor.
www.nature.com
"Time-resolved cryo-EM of G protein activation by a GPCR" #CryoEM #membrane #protein #structure #GPCR www.nature.com/articles/s41...
Reposted by Makaía Papasergi-Scott
Reposted by Makaía Papasergi-Scott
Happy New Year! (and first post here 😀) Excited to share the final version of our work where we combine deep mutational scanning, cryo-EM, and molecular dynamics to further resolve the mechanisms of pH sensing in GPCRs.
🧵(1/n)
www.cell.com/cell/fulltex...
🧵(1/n)
www.cell.com/cell/fulltex...
January 6, 2025 at 7:57 PM
Happy New Year! (and first post here 😀) Excited to share the final version of our work where we combine deep mutational scanning, cryo-EM, and molecular dynamics to further resolve the mechanisms of pH sensing in GPCRs.
🧵(1/n)
www.cell.com/cell/fulltex...
🧵(1/n)
www.cell.com/cell/fulltex...
Reposted by Makaía Papasergi-Scott
Elucidating the dynamics of Integrin αIIb[beta]3 from native platelet membranes by cryo-EM with build and retrieve method https://www.biorxiv.org/content/10.1101/2024.11.27.625729v1
Elucidating the dynamics of Integrin αIIb[beta]3 from native platelet membranes by cryo-EM with build and retrieve method https://www.biorxiv.org/content/10.1101/2024.11.27.625729v1
Platelets fulfill their essential physiological roles sensing the extracellular environment through
www.biorxiv.org
November 28, 2024 at 1:45 AM
Elucidating the dynamics of Integrin αIIb[beta]3 from native platelet membranes by cryo-EM with build and retrieve method https://www.biorxiv.org/content/10.1101/2024.11.27.625729v1
Reposted by Makaía Papasergi-Scott
Very excited to share the first story from my postdoc where we uncover the structural basis for slow Cas9 turnover!
We use kinetics-guided cryo-EM to capture 25 conformational states of SpCas9 from a single dataset - including the first look at Cas9 releasing product.
biorxiv.org/content/10.1...
We use kinetics-guided cryo-EM to capture 25 conformational states of SpCas9 from a single dataset - including the first look at Cas9 releasing product.
biorxiv.org/content/10.1...
November 28, 2024 at 12:32 PM
Very excited to share the first story from my postdoc where we uncover the structural basis for slow Cas9 turnover!
We use kinetics-guided cryo-EM to capture 25 conformational states of SpCas9 from a single dataset - including the first look at Cas9 releasing product.
biorxiv.org/content/10.1...
We use kinetics-guided cryo-EM to capture 25 conformational states of SpCas9 from a single dataset - including the first look at Cas9 releasing product.
biorxiv.org/content/10.1...
Reposted by Makaía Papasergi-Scott
We will be hosting 3rd Full Circle Symposium for Native Biologists hosted at UCSF in April 2025! We aim to build community and provide meaningful opportunities for Native scientists. If you are a Native biologist who is interested plz fill this out to stay up to date: forms.gle/AN7nfkhdB3Fv...
November 18, 2024 at 8:06 PM
We will be hosting 3rd Full Circle Symposium for Native Biologists hosted at UCSF in April 2025! We aim to build community and provide meaningful opportunities for Native scientists. If you are a Native biologist who is interested plz fill this out to stay up to date: forms.gle/AN7nfkhdB3Fv...
Reposted by Makaía Papasergi-Scott
Improved automated model building for cryo-EM maps using CryFold https://www.biorxiv.org/content/10.1101/2024.11.13.623164v1
Improved automated model building for cryo-EM maps using CryFold https://www.biorxiv.org/content/10.1101/2024.11.13.623164v1
Constructing atomic models from cryogenic electron microscopy (cryo-EM) density maps is essential fo
www.biorxiv.org
November 15, 2024 at 11:47 PM
Improved automated model building for cryo-EM maps using CryFold https://www.biorxiv.org/content/10.1101/2024.11.13.623164v1
Reposted by Makaía Papasergi-Scott
as a Big Nerd I have to tell you I went to Mendel’s abbey to look at his peas but the most notable part is actually the little hats he made for his microscopes
October 13, 2024 at 12:05 PM
as a Big Nerd I have to tell you I went to Mendel’s abbey to look at his peas but the most notable part is actually the little hats he made for his microscopes
Reposted by Makaía Papasergi-Scott
Do you know who Douglas Prasher is? Many don't, even though he is the person who cloned the original #GFP gene in the late 1980s. In my short history of plant light #microscopy I also cover a bit of his story - & why he is relatively unknown today, despite the importance of his work. See this 🧵👇
May 20, 2024 at 2:19 PM
Do you know who Douglas Prasher is? Many don't, even though he is the person who cloned the original #GFP gene in the late 1980s. In my short history of plant light #microscopy I also cover a bit of his story - & why he is relatively unknown today, despite the importance of his work. See this 🧵👇
Reposted by Makaía Papasergi-Scott
A certain researcher has had 3 papers retracted due to obvious manipulation as in this example below. Saying that scientists cannot push for retraction because a paper is already peer reviewed or should shut up as they never tried to republish something completely wrong is not a good look
November 8, 2024 at 3:49 PM
A certain researcher has had 3 papers retracted due to obvious manipulation as in this example below. Saying that scientists cannot push for retraction because a paper is already peer reviewed or should shut up as they never tried to republish something completely wrong is not a good look
Reposted by Makaía Papasergi-Scott
A first attempt to assemble GPCR aficionados here on 🦋 Let me know who I missed by replying below!
go.bsky.app/75ukb81
go.bsky.app/75ukb81
November 16, 2024 at 6:05 PM
A first attempt to assemble GPCR aficionados here on 🦋 Let me know who I missed by replying below!
go.bsky.app/75ukb81
go.bsky.app/75ukb81
Reposted by Makaía Papasergi-Scott
I am already running a service to notify about new #SerialEM releases by email at nexperion.net/serialemupdatenotices, but considering to make this a Bluesky bot as well ... is this something the #teamtomo #cryoEM #cryoET community would be interested in?
Nexperion - SerialEM Update Notices
Register here for a Nexperion Service to receive automatic email notifications once a SerialEM version is published.
nexperion.net
November 14, 2024 at 8:58 AM
I am already running a service to notify about new #SerialEM releases by email at nexperion.net/serialemupdatenotices, but considering to make this a Bluesky bot as well ... is this something the #teamtomo #cryoEM #cryoET community would be interested in?
Reposted by Makaía Papasergi-Scott
I kind of forgot how to announce Feeds, but here goes
For an all-in-one CryoEM Feed:
#CryoEM, #CryoET, #TeamTomo all-in-one feed that curates posts from the last 7 days containing the various cryoEM and cryoET related hashtags and keywords.
bsky.app/profile/did:...
For an all-in-one CryoEM Feed:
#CryoEM, #CryoET, #TeamTomo all-in-one feed that curates posts from the last 7 days containing the various cryoEM and cryoET related hashtags and keywords.
bsky.app/profile/did:...
November 9, 2024 at 3:14 AM
I kind of forgot how to announce Feeds, but here goes
For an all-in-one CryoEM Feed:
#CryoEM, #CryoET, #TeamTomo all-in-one feed that curates posts from the last 7 days containing the various cryoEM and cryoET related hashtags and keywords.
bsky.app/profile/did:...
For an all-in-one CryoEM Feed:
#CryoEM, #CryoET, #TeamTomo all-in-one feed that curates posts from the last 7 days containing the various cryoEM and cryoET related hashtags and keywords.
bsky.app/profile/did:...
"Time-resolved cryo-EM of G protein activation by a GPCR" #CryoEM #membrane #protein #structure #GPCR www.nature.com/articles/s41...
Time-resolved cryo-EM of G-protein activation by a GPCR - Nature
Time-resolved cryo-EM is used to capture structural transitions during G-protein activation stimulated by a G-protein-coupled receptor.
www.nature.com
March 17, 2024 at 9:28 PM
"Time-resolved cryo-EM of G protein activation by a GPCR" #CryoEM #membrane #protein #structure #GPCR www.nature.com/articles/s41...
Reposted by Makaía Papasergi-Scott
This one is for the #cryoEM afficionados. Ten years ago tomorrow (March 14th, 2014), I was at the Tahoe symposium on cryoEM 3D image analysis (taking place again this year, starting today!) and attended a talk by Alberto Bartesaghi, then from NCI-NIH now Duke.
March 13, 2024 at 3:39 PM
This one is for the #cryoEM afficionados. Ten years ago tomorrow (March 14th, 2014), I was at the Tahoe symposium on cryoEM 3D image analysis (taking place again this year, starting today!) and attended a talk by Alberto Bartesaghi, then from NCI-NIH now Duke.
Reposted by Makaía Papasergi-Scott
Interplay between Mg2+ and Ca2+ at multiple sites of the ryanodine receptor https://www.biorxiv.org/content/10.1101/2024.02.16.580716v1
Interplay between Mg2+ and Ca2+ at multiple sites of the ryanodine receptor https://www.biorxiv.org/content/10.1101/2024.02.16.580716v1
RyR1 is an intracellular Ca2+ channel important in excitable cells. Ca2+ activates it at low concent
www.biorxiv.org
February 21, 2024 at 12:49 PM
Interplay between Mg2+ and Ca2+ at multiple sites of the ryanodine receptor https://www.biorxiv.org/content/10.1101/2024.02.16.580716v1
Super cool study on the nicotinic receptor using time-resolved cryoEM! www.cell.com/cell/fulltex...
Structural mechanisms of α7 nicotinic receptor allosteric modulation and activation
Structural interrogation of α7 nicotinic receptor positive modulation clarifies class-specific
differences, reveals unique properties of the α7 subtype, and supports a conserved
potentiation mechanism...
www.cell.com
February 21, 2024 at 4:17 AM
Super cool study on the nicotinic receptor using time-resolved cryoEM! www.cell.com/cell/fulltex...
Reposted by Makaía Papasergi-Scott
Some rings for #FluorescenceFriday: spectrin labeling of the axonal periodic scaffold seen by 3D-STORM. Did you know that rings are synchronized between axons when bundle together? Now you do!
February 9, 2024 at 11:16 AM
Some rings for #FluorescenceFriday: spectrin labeling of the axonal periodic scaffold seen by 3D-STORM. Did you know that rings are synchronized between axons when bundle together? Now you do!
Reposted by Makaía Papasergi-Scott
Here's an exciting job for the right person.
Chance to join the senior staff at EMBO.
Based in Heidelberg at the EMBO headquarters, it's a great organisation and a great place to work.
www.embl.org/jobs/positio...
Chance to join the senior staff at EMBO.
Based in Heidelberg at the EMBO headquarters, it's a great organisation and a great place to work.
www.embl.org/jobs/positio...
January 24, 2024 at 5:06 PM
Here's an exciting job for the right person.
Chance to join the senior staff at EMBO.
Based in Heidelberg at the EMBO headquarters, it's a great organisation and a great place to work.
www.embl.org/jobs/positio...
Chance to join the senior staff at EMBO.
Based in Heidelberg at the EMBO headquarters, it's a great organisation and a great place to work.
www.embl.org/jobs/positio...
Reposted by Makaía Papasergi-Scott
Enlightening interview with David Gloriam about how to properly measure GPCR ligand bias.
youtube.com/watch?v=g5PX...
youtube.com/watch?v=g5PX...
Community guidelines for GPCR ligand bias: IUPHAR review 32 - interview with Prof David Gloriam
Read the Community guidelines for GPCR ligand bias: IUPHAR review 32: https://bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15811Browse the British Journal...
youtube.com
January 19, 2024 at 4:27 PM
Enlightening interview with David Gloriam about how to properly measure GPCR ligand bias.
youtube.com/watch?v=g5PX...
youtube.com/watch?v=g5PX...
Reposted by Makaía Papasergi-Scott
www.sciencedirect.com/science/arti...
Our Cell paper appeared today:
"Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling" --
New chip, described in detail + biol. application: intermediates at 10, 25, 140 ms.
Our Cell paper appeared today:
"Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling" --
New chip, described in detail + biol. application: intermediates at 10, 25, 140 ms.
January 19, 2024 at 3:55 PM
www.sciencedirect.com/science/arti...
Our Cell paper appeared today:
"Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling" --
New chip, described in detail + biol. application: intermediates at 10, 25, 140 ms.
Our Cell paper appeared today:
"Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling" --
New chip, described in detail + biol. application: intermediates at 10, 25, 140 ms.
Reposted by Makaía Papasergi-Scott
Science Writers: NASW is transitioning from Twitter to Bluesky as its short-form social media platform for community building. To facilitate Bluesky signups for our member network, we've created a code donation and receiving tool. See you all here soon! www.nasw.org/article/nasw... 💚
Transitioning from tweets, NASW migrates across to Bluesky
NASW is transitioning to Bluesky as its short-form social media platform for community building. Find out how NASW members can join the conversation.
www.nasw.org
January 2, 2024 at 4:48 PM
Science Writers: NASW is transitioning from Twitter to Bluesky as its short-form social media platform for community building. To facilitate Bluesky signups for our member network, we've created a code donation and receiving tool. See you all here soon! www.nasw.org/article/nasw... 💚