Masahiro Ono @ Tocky Lab
@monotockylab.bsky.social
Immunologist | Creator and Developer of Tocky | Integrative Experimental and Computational Immunology | T cells | Cancer Immunology | Immunotherapy
Imperial College London, Reader
Website: monotockylab.github.io
GitHub: github.com/MonoTockyLab
Imperial College London, Reader
Website: monotockylab.github.io
GitHub: github.com/MonoTockyLab
Pinned
🚀Thrilled to announce our new Tocky-CRISPR-Deep Learning paper!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
Reposted by Masahiro Ono @ Tocky Lab
Honoured that our T-cell immunotherapy research at Imperial is featured in The Telegraph via Cancer Research UK. Using Tocky, we reveal the behaviour of therapy-responsive and anti-cancer T cells, aiming to further improve immunotherapy approaches to melanoma.
www.telegraph.co.uk/science/canc...
www.telegraph.co.uk/science/canc...
How good bugs are fighting back against skin cancer
The answer lies in the microbiome. Read about the breakthrough research offering new hope
www.telegraph.co.uk
July 27, 2025 at 9:22 AM
Honoured that our T-cell immunotherapy research at Imperial is featured in The Telegraph via Cancer Research UK. Using Tocky, we reveal the behaviour of therapy-responsive and anti-cancer T cells, aiming to further improve immunotherapy approaches to melanoma.
www.telegraph.co.uk/science/canc...
www.telegraph.co.uk/science/canc...
Honoured that our T-cell immunotherapy research at Imperial is featured in The Telegraph via Cancer Research UK. Using Tocky, we reveal the behaviour of therapy-responsive and anti-cancer T cells, aiming to further improve immunotherapy approaches to melanoma.
www.telegraph.co.uk/science/canc...
www.telegraph.co.uk/science/canc...
How good bugs are fighting back against skin cancer
The answer lies in the microbiome. Read about the breakthrough research offering new hope
www.telegraph.co.uk
July 27, 2025 at 9:22 AM
Honoured that our T-cell immunotherapy research at Imperial is featured in The Telegraph via Cancer Research UK. Using Tocky, we reveal the behaviour of therapy-responsive and anti-cancer T cells, aiming to further improve immunotherapy approaches to melanoma.
www.telegraph.co.uk/science/canc...
www.telegraph.co.uk/science/canc...
🚀 Thrilled to share our new paper using Deep Learning and Tocky!
www.nature.com/articles/s41...
👉 We build on our original Tocky method by integrating CRISPR & deep learning to decode transcriptional dynamics.
🔥 7 years of work - toward data-driven immunology!
www.nature.com/articles/s41...
👉 We build on our original Tocky method by integrating CRISPR & deep learning to decode transcriptional dynamics.
🔥 7 years of work - toward data-driven immunology!
Machine learning-assisted decoding of temporal transcriptional dynamics via fluorescent timer - Nature Communications
Transcriptional dynamics govern gene regulation across development and immunity. Here, the authors combine CRISPR-engineered Timer reporter mice with machine learning to reveal age- and enhancer-depen...
www.nature.com
July 20, 2025 at 9:53 PM
🚀 Thrilled to share our new paper using Deep Learning and Tocky!
www.nature.com/articles/s41...
👉 We build on our original Tocky method by integrating CRISPR & deep learning to decode transcriptional dynamics.
🔥 7 years of work - toward data-driven immunology!
www.nature.com/articles/s41...
👉 We build on our original Tocky method by integrating CRISPR & deep learning to decode transcriptional dynamics.
🔥 7 years of work - toward data-driven immunology!
Reposted by Masahiro Ono @ Tocky Lab
🚀 Our next-generation cytometry tool GatingTree now published in #CytometryPartA!
🔗 onlinelibrary.wiley.com/doi/10.1002/...
GatingTree enables systematic analysis of high-dimensional flow cytometry data, without relying on dimensionality reduction. 💻
🔗 onlinelibrary.wiley.com/doi/10.1002/...
GatingTree enables systematic analysis of high-dimensional flow cytometry data, without relying on dimensionality reduction. 💻
GatingTree: Pathfinding Analysis of Group‐Specific Effects in Cytometry Data
Advancements in cytometry technologies have led to a remarkable increase in the number of markers that can be analyzed simultaneously, presenting significant challenges in data analysis. Traditional ....
onlinelibrary.wiley.com
July 13, 2025 at 12:24 PM
🚀 Our next-generation cytometry tool GatingTree now published in #CytometryPartA!
🔗 onlinelibrary.wiley.com/doi/10.1002/...
GatingTree enables systematic analysis of high-dimensional flow cytometry data, without relying on dimensionality reduction. 💻
🔗 onlinelibrary.wiley.com/doi/10.1002/...
GatingTree enables systematic analysis of high-dimensional flow cytometry data, without relying on dimensionality reduction. 💻
Reposted by Masahiro Ono @ Tocky Lab
🚀 New paper out!
Our ConvNet + Grad-CAM approach decodes Fluorescent Timer (Tocky) data at single-cell resolution.
Using Foxp3 Tocky, we reveal CNS2-controlled and developmental transcriptional dynamics.
Built on 7 years of work with AI and CRISPR!
www.nature.com/articles/s41...
Our ConvNet + Grad-CAM approach decodes Fluorescent Timer (Tocky) data at single-cell resolution.
Using Foxp3 Tocky, we reveal CNS2-controlled and developmental transcriptional dynamics.
Built on 7 years of work with AI and CRISPR!
www.nature.com/articles/s41...
Machine learning-assisted decoding of temporal transcriptional dynamics via fluorescent timer - Nature Communications
Transcriptional dynamics govern gene regulation across development and immunity. Here, the authors combine CRISPR-engineered Timer reporter mice with machine learning to reveal age- and enhancer-depen...
www.nature.com
July 2, 2025 at 8:42 AM
🚀 New paper out!
Our ConvNet + Grad-CAM approach decodes Fluorescent Timer (Tocky) data at single-cell resolution.
Using Foxp3 Tocky, we reveal CNS2-controlled and developmental transcriptional dynamics.
Built on 7 years of work with AI and CRISPR!
www.nature.com/articles/s41...
Our ConvNet + Grad-CAM approach decodes Fluorescent Timer (Tocky) data at single-cell resolution.
Using Foxp3 Tocky, we reveal CNS2-controlled and developmental transcriptional dynamics.
Built on 7 years of work with AI and CRISPR!
www.nature.com/articles/s41...
Reposted by Masahiro Ono @ Tocky Lab
Bored of black-box UMAP and clustering in #Cytometry?
GatingTree skips such methods, directly analyses high-dimensional data, builds reproducible tree paths across markers, and reveals group-specific effects.
No dimensionality reduction, just interpretable gates. 🌳
GatingTree skips such methods, directly analyses high-dimensional data, builds reproducible tree paths across markers, and reveals group-specific effects.
No dimensionality reduction, just interpretable gates. 🌳
July 13, 2025 at 12:25 PM
Bored of black-box UMAP and clustering in #Cytometry?
GatingTree skips such methods, directly analyses high-dimensional data, builds reproducible tree paths across markers, and reveals group-specific effects.
No dimensionality reduction, just interpretable gates. 🌳
GatingTree skips such methods, directly analyses high-dimensional data, builds reproducible tree paths across markers, and reveals group-specific effects.
No dimensionality reduction, just interpretable gates. 🌳
🚀 Our next-generation cytometry tool GatingTree now published in #CytometryPartA!
🔗 onlinelibrary.wiley.com/doi/10.1002/...
GatingTree enables systematic analysis of high-dimensional flow cytometry data, without relying on dimensionality reduction. 💻
🔗 onlinelibrary.wiley.com/doi/10.1002/...
GatingTree enables systematic analysis of high-dimensional flow cytometry data, without relying on dimensionality reduction. 💻
GatingTree: Pathfinding Analysis of Group‐Specific Effects in Cytometry Data
Advancements in cytometry technologies have led to a remarkable increase in the number of markers that can be analyzed simultaneously, presenting significant challenges in data analysis. Traditional ....
onlinelibrary.wiley.com
July 13, 2025 at 12:24 PM
🚀 Our next-generation cytometry tool GatingTree now published in #CytometryPartA!
🔗 onlinelibrary.wiley.com/doi/10.1002/...
GatingTree enables systematic analysis of high-dimensional flow cytometry data, without relying on dimensionality reduction. 💻
🔗 onlinelibrary.wiley.com/doi/10.1002/...
GatingTree enables systematic analysis of high-dimensional flow cytometry data, without relying on dimensionality reduction. 💻
Reposted by Masahiro Ono @ Tocky Lab
🚀Thrilled to announce our new Tocky-CRISPR-Deep Learning paper!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
July 5, 2025 at 8:31 PM
🚀Thrilled to announce our new Tocky-CRISPR-Deep Learning paper!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
🚀Thrilled to announce our new Tocky-CRISPR-Deep Learning paper!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
July 5, 2025 at 8:31 PM
🚀Thrilled to announce our new Tocky-CRISPR-Deep Learning paper!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
🚀 New paper out!
Our ConvNet + Grad-CAM approach decodes Fluorescent Timer (Tocky) data at single-cell resolution.
Using Foxp3 Tocky, we reveal CNS2-controlled and developmental transcriptional dynamics.
Built on 7 years of work with AI and CRISPR!
www.nature.com/articles/s41...
Our ConvNet + Grad-CAM approach decodes Fluorescent Timer (Tocky) data at single-cell resolution.
Using Foxp3 Tocky, we reveal CNS2-controlled and developmental transcriptional dynamics.
Built on 7 years of work with AI and CRISPR!
www.nature.com/articles/s41...
Machine learning-assisted decoding of temporal transcriptional dynamics via fluorescent timer - Nature Communications
Transcriptional dynamics govern gene regulation across development and immunity. Here, the authors combine CRISPR-engineered Timer reporter mice with machine learning to reveal age- and enhancer-depen...
www.nature.com
July 2, 2025 at 8:42 AM
🚀 New paper out!
Our ConvNet + Grad-CAM approach decodes Fluorescent Timer (Tocky) data at single-cell resolution.
Using Foxp3 Tocky, we reveal CNS2-controlled and developmental transcriptional dynamics.
Built on 7 years of work with AI and CRISPR!
www.nature.com/articles/s41...
Our ConvNet + Grad-CAM approach decodes Fluorescent Timer (Tocky) data at single-cell resolution.
Using Foxp3 Tocky, we reveal CNS2-controlled and developmental transcriptional dynamics.
Built on 7 years of work with AI and CRISPR!
www.nature.com/articles/s41...
Reposted by Masahiro Ono @ Tocky Lab
Excellent work from my long-term collaborator of over 17 years, @Sato_Y_Lab (on X) 🔬 on Runx/HTLV-1!
The start of our collaboration goes back to my own Runx paper in 2007: www.nature.com/articles/nat...
This Runx/HTLV-1 study is the latest milestone 🔬.
Honoured to be a co-author 📄✨
The start of our collaboration goes back to my own Runx paper in 2007: www.nature.com/articles/nat...
This Runx/HTLV-1 study is the latest milestone 🔬.
Honoured to be a co-author 📄✨
🚨Out now!
An intragenic open chromatin region in human T cell leukemia virus type 1 functions as a transcriptional silencer and regulates transcriptional burst by recruiting the host transcription factor RUNX.
#MicroSky 🧬🦠
www.nature.com/articles/s41...
An intragenic open chromatin region in human T cell leukemia virus type 1 functions as a transcriptional silencer and regulates transcriptional burst by recruiting the host transcription factor RUNX.
#MicroSky 🧬🦠
www.nature.com/articles/s41...
Intragenic viral silencer element regulates HTLV-1 latency via RUNX complex recruitment - Nature Microbiology
An intragenic open chromatin region in human T cell leukaemia virus type 1 functions as a transcriptional silencer and regulates transcriptional burst by recruiting the host transcription factor RUNX.
www.nature.com
May 17, 2025 at 4:41 PM
Excellent work from my long-term collaborator of over 17 years, @Sato_Y_Lab (on X) 🔬 on Runx/HTLV-1!
The start of our collaboration goes back to my own Runx paper in 2007: www.nature.com/articles/nat...
This Runx/HTLV-1 study is the latest milestone 🔬.
Honoured to be a co-author 📄✨
The start of our collaboration goes back to my own Runx paper in 2007: www.nature.com/articles/nat...
This Runx/HTLV-1 study is the latest milestone 🔬.
Honoured to be a co-author 📄✨
Excellent work from my long-term collaborator of over 17 years, @Sato_Y_Lab (on X) 🔬 on Runx/HTLV-1!
The start of our collaboration goes back to my own Runx paper in 2007: www.nature.com/articles/nat...
This Runx/HTLV-1 study is the latest milestone 🔬.
Honoured to be a co-author 📄✨
The start of our collaboration goes back to my own Runx paper in 2007: www.nature.com/articles/nat...
This Runx/HTLV-1 study is the latest milestone 🔬.
Honoured to be a co-author 📄✨
🚨Out now!
An intragenic open chromatin region in human T cell leukemia virus type 1 functions as a transcriptional silencer and regulates transcriptional burst by recruiting the host transcription factor RUNX.
#MicroSky 🧬🦠
www.nature.com/articles/s41...
An intragenic open chromatin region in human T cell leukemia virus type 1 functions as a transcriptional silencer and regulates transcriptional burst by recruiting the host transcription factor RUNX.
#MicroSky 🧬🦠
www.nature.com/articles/s41...
Intragenic viral silencer element regulates HTLV-1 latency via RUNX complex recruitment - Nature Microbiology
An intragenic open chromatin region in human T cell leukaemia virus type 1 functions as a transcriptional silencer and regulates transcriptional burst by recruiting the host transcription factor RUNX.
www.nature.com
May 17, 2025 at 4:41 PM
Excellent work from my long-term collaborator of over 17 years, @Sato_Y_Lab (on X) 🔬 on Runx/HTLV-1!
The start of our collaboration goes back to my own Runx paper in 2007: www.nature.com/articles/nat...
This Runx/HTLV-1 study is the latest milestone 🔬.
Honoured to be a co-author 📄✨
The start of our collaboration goes back to my own Runx paper in 2007: www.nature.com/articles/nat...
This Runx/HTLV-1 study is the latest milestone 🔬.
Honoured to be a co-author 📄✨
Reposted by Masahiro Ono @ Tocky Lab
What if Tregs are not a separate lineage, but part of spontaneously activated T cells?
In this work, we integrate this idea with our recent findings: Adult T cell leukemia (ATL) is not 'Treg cancer', challenging the current concept of Treg as a distinct entity.🧵(1/4)
academic.oup.com/discovimmuno...
In this work, we integrate this idea with our recent findings: Adult T cell leukemia (ATL) is not 'Treg cancer', challenging the current concept of Treg as a distinct entity.🧵(1/4)
academic.oup.com/discovimmuno...
Spectrum of Treg and self-reactive T cells: single cell perspectives from old friend HTLV-1
Summary. Despite extensive regulatory T cell (Treg) research, fundamental questions on in vivo dynamics remain to be answered. The current study aims to di
academic.oup.com
April 26, 2025 at 12:22 PM
What if Tregs are not a separate lineage, but part of spontaneously activated T cells?
In this work, we integrate this idea with our recent findings: Adult T cell leukemia (ATL) is not 'Treg cancer', challenging the current concept of Treg as a distinct entity.🧵(1/4)
academic.oup.com/discovimmuno...
In this work, we integrate this idea with our recent findings: Adult T cell leukemia (ATL) is not 'Treg cancer', challenging the current concept of Treg as a distinct entity.🧵(1/4)
academic.oup.com/discovimmuno...
What if Tregs are not a separate lineage, but part of spontaneously activated T cells?
In this work, we integrate this idea with our recent findings: Adult T cell leukemia (ATL) is not 'Treg cancer', challenging the current concept of Treg as a distinct entity.🧵(1/4)
academic.oup.com/discovimmuno...
In this work, we integrate this idea with our recent findings: Adult T cell leukemia (ATL) is not 'Treg cancer', challenging the current concept of Treg as a distinct entity.🧵(1/4)
academic.oup.com/discovimmuno...
Spectrum of Treg and self-reactive T cells: single cell perspectives from old friend HTLV-1
Summary. Despite extensive regulatory T cell (Treg) research, fundamental questions on in vivo dynamics remain to be answered. The current study aims to di
academic.oup.com
April 26, 2025 at 12:22 PM
What if Tregs are not a separate lineage, but part of spontaneously activated T cells?
In this work, we integrate this idea with our recent findings: Adult T cell leukemia (ATL) is not 'Treg cancer', challenging the current concept of Treg as a distinct entity.🧵(1/4)
academic.oup.com/discovimmuno...
In this work, we integrate this idea with our recent findings: Adult T cell leukemia (ATL) is not 'Treg cancer', challenging the current concept of Treg as a distinct entity.🧵(1/4)
academic.oup.com/discovimmuno...
Reposted by Masahiro Ono @ Tocky Lab
Explore how HTLV-1 hijacks self-reactive T cells via Periodic TCR signalling - a fresh lens on Treg biology and immune regulation. Game-changing insights from single-cell analysis. 👇
📄 Ono & Satou (2024)
🧬 bit.ly/42gRcuR
📄 Ono & Satou (2024)
🧬 bit.ly/42gRcuR
April 25, 2025 at 1:18 PM
Explore how HTLV-1 hijacks self-reactive T cells via Periodic TCR signalling - a fresh lens on Treg biology and immune regulation. Game-changing insights from single-cell analysis. 👇
📄 Ono & Satou (2024)
🧬 bit.ly/42gRcuR
📄 Ono & Satou (2024)
🧬 bit.ly/42gRcuR
Reposted by Masahiro Ono @ Tocky Lab
🔍 New Blog Post: The Dogma of Treg and The True Origins of Tocky
monotockylab.github.io/blog/2025/As...
The blog explores what is certain in Treg immunology and what is not, unraveling the past two decades of my research life that have been triggered by the non-reproducible evidence in Treg biology.
monotockylab.github.io/blog/2025/As...
The blog explores what is certain in Treg immunology and what is not, unraveling the past two decades of my research life that have been triggered by the non-reproducible evidence in Treg biology.
Masahiro Ono | The Dogma of Treg and The True Origins of Tocky
What is Preventing Advancements and Whats is Required for the Future?
monotockylab.github.io
March 24, 2025 at 10:51 PM
🔍 New Blog Post: The Dogma of Treg and The True Origins of Tocky
monotockylab.github.io/blog/2025/As...
The blog explores what is certain in Treg immunology and what is not, unraveling the past two decades of my research life that have been triggered by the non-reproducible evidence in Treg biology.
monotockylab.github.io/blog/2025/As...
The blog explores what is certain in Treg immunology and what is not, unraveling the past two decades of my research life that have been triggered by the non-reproducible evidence in Treg biology.
Reposted by Masahiro Ono @ Tocky Lab
Reposted by Masahiro Ono @ Tocky Lab
Single-cell analysis reveals how periodic TCR signalling defines the spectrum of Treg and self-reactive T cells—and how HTLV-1 may hijack this for immune evasion and transformation.
The study fills key gaps in Treg biology and viral #immunology.
🔗 bit.ly/42gRcuR
The study fills key gaps in Treg biology and viral #immunology.
🔗 bit.ly/42gRcuR
April 4, 2025 at 3:48 PM
Single-cell analysis reveals how periodic TCR signalling defines the spectrum of Treg and self-reactive T cells—and how HTLV-1 may hijack this for immune evasion and transformation.
The study fills key gaps in Treg biology and viral #immunology.
🔗 bit.ly/42gRcuR
The study fills key gaps in Treg biology and viral #immunology.
🔗 bit.ly/42gRcuR
🔍 New Blog Post: The Dogma of Treg and The True Origins of Tocky
monotockylab.github.io/blog/2025/As...
The blog explores what is certain in Treg immunology and what is not, unraveling the past two decades of my research life that have been triggered by the non-reproducible evidence in Treg biology.
monotockylab.github.io/blog/2025/As...
The blog explores what is certain in Treg immunology and what is not, unraveling the past two decades of my research life that have been triggered by the non-reproducible evidence in Treg biology.
Masahiro Ono | The Dogma of Treg and The True Origins of Tocky
What is Preventing Advancements and Whats is Required for the Future?
monotockylab.github.io
March 24, 2025 at 10:51 PM
🔍 New Blog Post: The Dogma of Treg and The True Origins of Tocky
monotockylab.github.io/blog/2025/As...
The blog explores what is certain in Treg immunology and what is not, unraveling the past two decades of my research life that have been triggered by the non-reproducible evidence in Treg biology.
monotockylab.github.io/blog/2025/As...
The blog explores what is certain in Treg immunology and what is not, unraveling the past two decades of my research life that have been triggered by the non-reproducible evidence in Treg biology.
Reposted by Masahiro Ono @ Tocky Lab
🌟 Excited to share my new publication on TockyPrep, now published in BMC Bioinformatics🚀 link.springer.com/article/10.1...
The TockyPrep R package automates and standardizes flow cytometric analysis of Fluorescent Timer reporters, unlocking the analysis of Nr4a3 Tocky mice and other Timer reporters🐭
The TockyPrep R package automates and standardizes flow cytometric analysis of Fluorescent Timer reporters, unlocking the analysis of Nr4a3 Tocky mice and other Timer reporters🐭
TockyPrep: data preprocessing methods for flow cytometric fluorescent timer analysis - BMC Bioinformatics
Background: Fluorescent Timer proteins, which display time-dependent changes in their emission spectra, are invaluable for analyzing the temporal dynamics of cellular events at the single-cell level. ...
link.springer.com
February 8, 2025 at 7:50 PM
🌟 Excited to share my new publication on TockyPrep, now published in BMC Bioinformatics🚀 link.springer.com/article/10.1...
The TockyPrep R package automates and standardizes flow cytometric analysis of Fluorescent Timer reporters, unlocking the analysis of Nr4a3 Tocky mice and other Timer reporters🐭
The TockyPrep R package automates and standardizes flow cytometric analysis of Fluorescent Timer reporters, unlocking the analysis of Nr4a3 Tocky mice and other Timer reporters🐭
🌟 Excited to share my new publication on TockyPrep, now published in BMC Bioinformatics🚀 link.springer.com/article/10.1...
The TockyPrep R package automates and standardizes flow cytometric analysis of Fluorescent Timer reporters, unlocking the analysis of Nr4a3 Tocky mice and other Timer reporters🐭
The TockyPrep R package automates and standardizes flow cytometric analysis of Fluorescent Timer reporters, unlocking the analysis of Nr4a3 Tocky mice and other Timer reporters🐭
TockyPrep: data preprocessing methods for flow cytometric fluorescent timer analysis - BMC Bioinformatics
Background: Fluorescent Timer proteins, which display time-dependent changes in their emission spectra, are invaluable for analyzing the temporal dynamics of cellular events at the single-cell level. ...
link.springer.com
February 8, 2025 at 7:50 PM
🌟 Excited to share my new publication on TockyPrep, now published in BMC Bioinformatics🚀 link.springer.com/article/10.1...
The TockyPrep R package automates and standardizes flow cytometric analysis of Fluorescent Timer reporters, unlocking the analysis of Nr4a3 Tocky mice and other Timer reporters🐭
The TockyPrep R package automates and standardizes flow cytometric analysis of Fluorescent Timer reporters, unlocking the analysis of Nr4a3 Tocky mice and other Timer reporters🐭
Pleased to announce TockyPrep update - the R package for preprocessing flow cytometric Fluorescent Timer data 🚀
monotockylab.github.io/TockyPrep/in...
Now featuring an Interactive application to fine-tune your analysis! 🛠️
monotockylab.github.io/TockyPrep/ar...
monotockylab.github.io/TockyPrep/in...
Now featuring an Interactive application to fine-tune your analysis! 🛠️
monotockylab.github.io/TockyPrep/ar...
January 18, 2025 at 8:14 AM
Pleased to announce TockyPrep update - the R package for preprocessing flow cytometric Fluorescent Timer data 🚀
monotockylab.github.io/TockyPrep/in...
Now featuring an Interactive application to fine-tune your analysis! 🛠️
monotockylab.github.io/TockyPrep/ar...
monotockylab.github.io/TockyPrep/in...
Now featuring an Interactive application to fine-tune your analysis! 🛠️
monotockylab.github.io/TockyPrep/ar...
Reposted by Masahiro Ono @ Tocky Lab
A multidimensional toolkit for elucidating temporal trajectories in cell development in vivo
Read this Techniques and Resources Article by Masahiro Ono @monotockylab.bsky.social and Tessa Crompton:
https://doi.org/10.1242/dev.204255
Read this Techniques and Resources Article by Masahiro Ono @monotockylab.bsky.social and Tessa Crompton:
https://doi.org/10.1242/dev.204255
December 29, 2024 at 9:51 AM
A multidimensional toolkit for elucidating temporal trajectories in cell development in vivo
Read this Techniques and Resources Article by Masahiro Ono @monotockylab.bsky.social and Tessa Crompton:
https://doi.org/10.1242/dev.204255
Read this Techniques and Resources Article by Masahiro Ono @monotockylab.bsky.social and Tessa Crompton:
https://doi.org/10.1242/dev.204255
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 20
#Books
#BookSky💙📚
#BookChallenge
Day 20
December 25, 2024 at 6:57 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 20
#Books
#BookSky💙📚
#BookChallenge
Day 20