Matteo Gravino
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matteogravino.bsky.social
Matteo Gravino
@matteogravino.bsky.social
35 followers 37 following 9 posts
Scientist at the John Innes Centre. Interested in plant-aphid interactions.
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matteogravino.bsky.social
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🌟Congrats to the amazing team:
@matteogravino.bsky.social
@samtmugford.bsky.social
Daniela Pontiggia
Joshua Joyce
Claire Drurey
David Prince
Felice Cervone
Giulia De Lorenzo
@saskiahogenhout.bsky.social

🙏Thanks to editor Carlos Ballaré & referees for their valuable feedback!
August 1, 2025 at 12:40 PM
matteogravino.bsky.social
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🧬Our model also includes recent findings:

· Mp10’s immune suppression involves interaction with AMSH deubiquitinases
👉 Matteo Gravino and @samtmugford.bsky.social work: bsky.app/profile/samt...

· Other aphid effectors actively target EDS1
👉 @qunliu07.bsky.social's work: bsky.app/profile/qunl...
August 1, 2025 at 12:40 PM
matteogravino.bsky.social
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🔑 Conclusion: EDS1 mediates Mp10's dual function—suppressing DTI/PTI and triggering ETI.

🧩 Mp10 operates at the crossroads of PTI/DTI and ETI, manipulating plant immunity to the aphid’s advantage.
Model: Activation and suppression of defences in plant–aphid interactions. Upper left panel: aphid stylet penetration of the cell wall releases oligogalacturonides (OGs), which induce PAMP/DAMP-triggered immunity (PTI/DTI) in a process dependent on BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1 (BAK1), ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), and CPK5/6/11 (data herein). The stabilisation of pattern recognition receptors (PRRs) and coreceptors at the plasma membrane may rely on the deubiquitination (DUB) activity of ASSOCIATED MOLECULE WITH THE SH3 DOMAIN OF STAM (AMSHs; Gravino et al., 2024). Upper right panel: the Mp10 effector, introduced by aphids into the cell cytoplasm (Mugford et al., 2016), suppresses OG-induced reactive oxygen species (ROS) (data herein) and flg22-induced PTI (Bos et al., 2010). Mp10 targeting of plant AMSHs is implicated in these processes (Gravino et al., 2024). EDS1 is essential for Mp10-mediated ROS suppression and PRR destabilisation (data herein), acting through an unidentified mechanism (denoted by the double-sided arrow with an asterisk). Lower left panel: effector-triggered immunity (ETI) is activated through EDS1, either directly or indirectly, upon recognition of Mp10 and/or its activities by a TNL (Gravino et al., 2024; Rao et al., 2024). Salicylic acid glucosyltransferase 1 (SGT1) is also required for TNL/ETI activation (Bos et al., 2010). Lower right panel: aphids secrete additional effectors, such as cathepsin B proteins (e.g. CathB6), which target EDS1 to suppress ETI (Liu et al., 2025). Solid arrows, increased activation; solid blunt-ended arrows, increased suppression; Dashed arrows, reduced activation; Dashed -blunt-ended arrows, reduced suppression.
August 1, 2025 at 12:40 PM
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We found Mp10's effect is EDS1-dependent.
Without EDS1:
· No ETI
· PRRs stabilize
· DTI/PTI aren't suppressed
⏩ Aphids colonise more easily
August 1, 2025 at 12:40 PM
matteogravino.bsky.social
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However, Mp10 also triggers ETI-like responses, including chlorosis—ultimately reducing aphid success. Why?
August 1, 2025 at 12:40 PM
matteogravino.bsky.social
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But aphids fight back. How?

🛡️ They suppress OG production upon wounding

🧬 And deploy Mp10, an aphid-secreted saliva effector protein that:
· Reduces OG/flg22 sensitivity
· Destabilises pattern recognition receptors (PRRs)
August 1, 2025 at 12:40 PM
matteogravino.bsky.social
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💥 DTI—triggered by the plant cell-wall-derived fragments oligogalacturonides (OGs)—strongly limits aphid colonisation. This defence requires:
· BAK1/BKK1
· CPK5/6/11
· GRP3
· EDS1
August 1, 2025 at 12:40 PM
matteogravino.bsky.social
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We explored how the polyphagous peach–potato aphid (Myzus persicae) colonises Arabidopsis thaliana, and uncovered a key role for DAMP-triggered immunity (DTI).
Peach–potato aphid (Myzus persicae)
August 1, 2025 at 12:40 PM