Marie Winz
@mariewinz.bsky.social
RNA biologist at Johannes Gutenberg University Mainz, Germany: co-translational quality control - RNA modification - and beyond.
Lab url: ak-winz.pharmazie.uni-mainz.de
Lab url: ak-winz.pharmazie.uni-mainz.de
Curiously, Jlp2 acts not only on peptidyl-tRNA on large ribosomal subunits, but also on full monosomes. However, we also show that Jlp2 requires ribosome splitting for activity, suggesting that the monosomes represent re-assembled subunits. Read our preprint for full story!
September 9, 2025 at 6:17 PM
Curiously, Jlp2 acts not only on peptidyl-tRNA on large ribosomal subunits, but also on full monosomes. However, we also show that Jlp2 requires ribosome splitting for activity, suggesting that the monosomes represent re-assembled subunits. Read our preprint for full story!
Instead, we find that Jlp2 acts as a release factor enabling peptide release from ribosome-bound peptidyl-tRNA. We show that Jlp2 acts independently of the RQC complex and of other, known or expected release factors. Through peptide release, Jlp2 maintains cellular fitness in translation stress.
September 9, 2025 at 6:17 PM
Instead, we find that Jlp2 acts as a release factor enabling peptide release from ribosome-bound peptidyl-tRNA. We show that Jlp2 acts independently of the RQC complex and of other, known or expected release factors. Through peptide release, Jlp2 maintains cellular fitness in translation stress.
We identify yeast Jlp2 as a translation quality control factor that regulates CAT tailing upon RQC dysfunction, counteracting CAT tail-dependent protein aggregation. However, NOT by physically competing with or influencing abundance or function of Rqc2, the factor that catalyzes CAT tailing.
September 9, 2025 at 6:17 PM
We identify yeast Jlp2 as a translation quality control factor that regulates CAT tailing upon RQC dysfunction, counteracting CAT tail-dependent protein aggregation. However, NOT by physically competing with or influencing abundance or function of Rqc2, the factor that catalyzes CAT tailing.
Stalled or collided ribosomes are split during quality control, leaving aberrant nascent peptides conjugated to tRNA bound to large ribosomal subunits. Those peptides are targeted by #RQC, and ubiquitinated and/or CAT tailed to facilitate degradation. If RQC fails, who comes to the rescue?
September 9, 2025 at 6:17 PM
Stalled or collided ribosomes are split during quality control, leaving aberrant nascent peptides conjugated to tRNA bound to large ribosomal subunits. Those peptides are targeted by #RQC, and ubiquitinated and/or CAT tailed to facilitate degradation. If RQC fails, who comes to the rescue?
4. Backtracked RNA can be polyadenylated (AAA) on the 3' end by the TRAMP complex. We speculate that AAA-marked nascent RNA could either be degraded or trigger reverse torpedo termination.
Although this work focuses on RNAPI, many findings apply broadly to RNAPs
Although this work focuses on RNAPI, many findings apply broadly to RNAPs
February 25, 2025 at 6:36 PM
4. Backtracked RNA can be polyadenylated (AAA) on the 3' end by the TRAMP complex. We speculate that AAA-marked nascent RNA could either be degraded or trigger reverse torpedo termination.
Although this work focuses on RNAPI, many findings apply broadly to RNAPs
Although this work focuses on RNAPI, many findings apply broadly to RNAPs
3. We proposed an alternative model for torpedo termination, where a 5'→3' exonuclease trims the nascent RNA to RNAP boundaries, followed by backtracking-mediated cleavage.
February 25, 2025 at 6:35 PM
3. We proposed an alternative model for torpedo termination, where a 5'→3' exonuclease trims the nascent RNA to RNAP boundaries, followed by backtracking-mediated cleavage.
2. We uncovered multiple mechanisms contributing to RNAPI transcription termination, including road-block termination, end-nucleolytic cleavage, and Rat1 torpedo termination.
February 25, 2025 at 6:34 PM
2. We uncovered multiple mechanisms contributing to RNAPI transcription termination, including road-block termination, end-nucleolytic cleavage, and Rat1 torpedo termination.
Summary by Tomasz:
Some key takeaways:
1. The first experimental evidence supporting the model of immobilized RNAPs and spinning DNA—validated by experimental confirmation of theoretical predictions.
Some key takeaways:
1. The first experimental evidence supporting the model of immobilized RNAPs and spinning DNA—validated by experimental confirmation of theoretical predictions.
February 25, 2025 at 6:34 PM
Summary by Tomasz:
Some key takeaways:
1. The first experimental evidence supporting the model of immobilized RNAPs and spinning DNA—validated by experimental confirmation of theoretical predictions.
Some key takeaways:
1. The first experimental evidence supporting the model of immobilized RNAPs and spinning DNA—validated by experimental confirmation of theoretical predictions.