TJ Krzystek
@lakeerieneuro.bsky.social
PhD in #CellBiology || Senior Scientist 🔬
#Microscopy #Neuroscience #DiseaseModeling #hiPSCs
#Phenomics #TargetBiology #DrugDiscovery #LiveImaging
#BillsMafia || Posts represent only myself
#Microscopy #Neuroscience #DiseaseModeling #hiPSCs
#Phenomics #TargetBiology #DrugDiscovery #LiveImaging
#BillsMafia || Posts represent only myself
appreciate the compliment, Rachael!
December 20, 2024 at 9:09 PM
appreciate the compliment, Rachael!
caption/alt:
iPSC-derived spinal motor neurons from a control/healthy individual were aged 32 days, probed with Spirochrome's pkMito mitochondria dye, then assayed 24hrs later on our automated imager.
The sequence depicted was sped up to 20fps,
but images were capture every 4s across 5mins.
iPSC-derived spinal motor neurons from a control/healthy individual were aged 32 days, probed with Spirochrome's pkMito mitochondria dye, then assayed 24hrs later on our automated imager.
The sequence depicted was sped up to 20fps,
but images were capture every 4s across 5mins.
December 20, 2024 at 6:06 PM
caption/alt:
iPSC-derived spinal motor neurons from a control/healthy individual were aged 32 days, probed with Spirochrome's pkMito mitochondria dye, then assayed 24hrs later on our automated imager.
The sequence depicted was sped up to 20fps,
but images were capture every 4s across 5mins.
iPSC-derived spinal motor neurons from a control/healthy individual were aged 32 days, probed with Spirochrome's pkMito mitochondria dye, then assayed 24hrs later on our automated imager.
The sequence depicted was sped up to 20fps,
but images were capture every 4s across 5mins.
I can watch the ebb-and-flow of that leading edge all day!
December 20, 2024 at 2:51 PM
I can watch the ebb-and-flow of that leading edge all day!
That'll be a really interesting comparison! Mitochondria movement is quite challenging to predict with shorter timelapses like this.
Not sure how much it applies beyond axonal transport, but lysosomes and other vesicles always give me less noisy and more linear particle tracking data.
Not sure how much it applies beyond axonal transport, but lysosomes and other vesicles always give me less noisy and more linear particle tracking data.
December 17, 2024 at 2:55 PM
That'll be a really interesting comparison! Mitochondria movement is quite challenging to predict with shorter timelapses like this.
Not sure how much it applies beyond axonal transport, but lysosomes and other vesicles always give me less noisy and more linear particle tracking data.
Not sure how much it applies beyond axonal transport, but lysosomes and other vesicles always give me less noisy and more linear particle tracking data.
Much appreciated 🫡
December 16, 2024 at 10:44 PM
Much appreciated 🫡
It's also #FluorescenceFriday 🔬
so, here's a taste of the lysosomal transport you can find at my poster (HCB-23)
At TDI, we've been integrating #microscopy and #phenomics to identify and validate phenotypes of ALS patient-derived spinal motor neurons for subsequent drug screening.
so, here's a taste of the lysosomal transport you can find at my poster (HCB-23)
At TDI, we've been integrating #microscopy and #phenomics to identify and validate phenotypes of ALS patient-derived spinal motor neurons for subsequent drug screening.
December 6, 2024 at 11:58 AM
It's also #FluorescenceFriday 🔬
so, here's a taste of the lysosomal transport you can find at my poster (HCB-23)
At TDI, we've been integrating #microscopy and #phenomics to identify and validate phenotypes of ALS patient-derived spinal motor neurons for subsequent drug screening.
so, here's a taste of the lysosomal transport you can find at my poster (HCB-23)
At TDI, we've been integrating #microscopy and #phenomics to identify and validate phenotypes of ALS patient-derived spinal motor neurons for subsequent drug screening.
At HCB-24,
Anushka B. will share a novel TDP-43 KO cell line for disease modeling.
At HCB-14,
Therese D. will share how C9orf72 HRE or LoF impacts TDP-43 mis-splicing.
At HCB-23,
I'll share our design & development of an automated, live-cell imaging pipeline for drug screening and phenomics.
Anushka B. will share a novel TDP-43 KO cell line for disease modeling.
At HCB-14,
Therese D. will share how C9orf72 HRE or LoF impacts TDP-43 mis-splicing.
At HCB-23,
I'll share our design & development of an automated, live-cell imaging pipeline for drug screening and phenomics.
December 6, 2024 at 11:58 AM
At HCB-24,
Anushka B. will share a novel TDP-43 KO cell line for disease modeling.
At HCB-14,
Therese D. will share how C9orf72 HRE or LoF impacts TDP-43 mis-splicing.
At HCB-23,
I'll share our design & development of an automated, live-cell imaging pipeline for drug screening and phenomics.
Anushka B. will share a novel TDP-43 KO cell line for disease modeling.
At HCB-14,
Therese D. will share how C9orf72 HRE or LoF impacts TDP-43 mis-splicing.
At HCB-23,
I'll share our design & development of an automated, live-cell imaging pipeline for drug screening and phenomics.
I curated more new works here: biomed.news/bims-axbals/...
This collection focuses on recent studies related to axonal biology, cell biology of neurons, neurodegenerative diseases, and some iPSC biology.
@gavinmcstay.bsky.social @biomednews.bsky.social
#Neuroscience #CellBiology #AxonalBiology
This collection focuses on recent studies related to axonal biology, cell biology of neurons, neurodegenerative diseases, and some iPSC biology.
@gavinmcstay.bsky.social @biomednews.bsky.social
#Neuroscience #CellBiology #AxonalBiology
December 2, 2024 at 12:46 PM
I curated more new works here: biomed.news/bims-axbals/...
This collection focuses on recent studies related to axonal biology, cell biology of neurons, neurodegenerative diseases, and some iPSC biology.
@gavinmcstay.bsky.social @biomednews.bsky.social
#Neuroscience #CellBiology #AxonalBiology
This collection focuses on recent studies related to axonal biology, cell biology of neurons, neurodegenerative diseases, and some iPSC biology.
@gavinmcstay.bsky.social @biomednews.bsky.social
#Neuroscience #CellBiology #AxonalBiology
ImageJ Fiji 💯
Adding setBatchMode(true) saves a lot of time if you're using a custom macro/script.
I've been processing tifs from 384-well plates with 180-360 frames/well.
Adding setBatchMode(true) saves a lot of time if you're using a custom macro/script.
I've been processing tifs from 384-well plates with 180-360 frames/well.
November 28, 2024 at 10:02 AM
ImageJ Fiji 💯
Adding setBatchMode(true) saves a lot of time if you're using a custom macro/script.
I've been processing tifs from 384-well plates with 180-360 frames/well.
Adding setBatchMode(true) saves a lot of time if you're using a custom macro/script.
I've been processing tifs from 384-well plates with 180-360 frames/well.
The alt text disappeared on me 😱
Here it is:
iPSC-derived motor neurons (labeled green with a membrane dye) were treated with four concentrations of an exogenous stressor (0uM, 0.01uM, 0.1uM, 1.0uM) and imaged every 6 hours across 54 hours using our automated spinning disk.
Scale = 100microns
Here it is:
iPSC-derived motor neurons (labeled green with a membrane dye) were treated with four concentrations of an exogenous stressor (0uM, 0.01uM, 0.1uM, 1.0uM) and imaged every 6 hours across 54 hours using our automated spinning disk.
Scale = 100microns
November 28, 2024 at 9:09 AM
The alt text disappeared on me 😱
Here it is:
iPSC-derived motor neurons (labeled green with a membrane dye) were treated with four concentrations of an exogenous stressor (0uM, 0.01uM, 0.1uM, 1.0uM) and imaged every 6 hours across 54 hours using our automated spinning disk.
Scale = 100microns
Here it is:
iPSC-derived motor neurons (labeled green with a membrane dye) were treated with four concentrations of an exogenous stressor (0uM, 0.01uM, 0.1uM, 1.0uM) and imaged every 6 hours across 54 hours using our automated spinning disk.
Scale = 100microns