IMAGE Lab
@labphan.bsky.social
B cells | Macrophages | Immunity | Autoimmunity | Cancer | Osteoclasts | Osteomorphs | Intravital imaging | Precision Immunology
Very excited at the chance to work with new people with new ideas in immunology! Really keen to hear from people from diverse backgrounds with different ways of thinking. International applicants seeking freedom to pursue BlueSky projects welcome. Come for the science. Stay for the vibe!
#immunosky
#immunosky
July 10, 2025 at 1:36 AM
Very excited at the chance to work with new people with new ideas in immunology! Really keen to hear from people from diverse backgrounds with different ways of thinking. International applicants seeking freedom to pursue BlueSky projects welcome. Come for the science. Stay for the vibe!
#immunosky
#immunosky
But sc data is noisy and nonlinear. AAnet takes archetype analysis to the next level by using an autoencoder to transform the data into a simplicial latent space. AAnet more accurately defines heterogeneity within cellular clusters & druggable transcriptional programs that drive heterogeneity. 3/3
June 26, 2025 at 11:38 AM
But sc data is noisy and nonlinear. AAnet takes archetype analysis to the next level by using an autoencoder to transform the data into a simplicial latent space. AAnet more accurately defines heterogeneity within cellular clusters & druggable transcriptional programs that drive heterogeneity. 3/3
This was associated with more rapid secretion of neutralising antibodies against the ancestral strain in the vaccine as well as Delta and Omicron variants. In agreement, there was more somatic hypermutation & antibody diversification in the boosted dLN compared to ndLN.
April 29, 2025 at 1:20 PM
This was associated with more rapid secretion of neutralising antibodies against the ancestral strain in the vaccine as well as Delta and Omicron variants. In agreement, there was more somatic hypermutation & antibody diversification in the boosted dLN compared to ndLN.
Please note caveats about persistent GCs from the COVID mRNA vaccine (Turner, Ellebedy et al. Nature 2021). Regardless, we did see more GCs in the boosted dLN compared to ndLN
April 29, 2025 at 1:20 PM
Please note caveats about persistent GCs from the COVID mRNA vaccine (Turner, Ellebedy et al. Nature 2021). Regardless, we did see more GCs in the boosted dLN compared to ndLN
This was all done in inbred genetically modified mice using intravital microscopy. Could boosting the same versus opposite side make a difference in 'wildtype' 'outbred' humans? This is where the Kirby Institute team came into their own! In the clinical study they also sampled the LN by FNB!
April 29, 2025 at 1:20 PM
This was all done in inbred genetically modified mice using intravital microscopy. Could boosting the same versus opposite side make a difference in 'wildtype' 'outbred' humans? This is where the Kirby Institute team came into their own! In the clinical study they also sampled the LN by FNB!
What about the SSMs? To test if vaccine had 'primed' the dLN SSMs we synchronously immunised the ndLN with an irrelevant non-cognate antigen. Now when we boost the 'ndLN' with cognate antigen there was larger recall & more GCs, just like dLN! This effect was abolished by anti-CSF1R mAb blocking.
April 29, 2025 at 1:20 PM
What about the SSMs? To test if vaccine had 'primed' the dLN SSMs we synchronously immunised the ndLN with an irrelevant non-cognate antigen. Now when we boost the 'ndLN' with cognate antigen there was larger recall & more GCs, just like dLN! This effect was abolished by anti-CSF1R mAb blocking.
Rama did scRNAseq & showed that dLN Bmems were distinct from ndLN Bmems. Upregulated dLN genes were involved in cell adhesion, migration & localisation, BCR activation & GC formation. Upregulated ndLN genes were involved in cell migration, tissue residence & plasma cell vs GC differentiation.
April 29, 2025 at 1:20 PM
Rama did scRNAseq & showed that dLN Bmems were distinct from ndLN Bmems. Upregulated dLN genes were involved in cell adhesion, migration & localisation, BCR activation & GC formation. Upregulated ndLN genes were involved in cell migration, tissue residence & plasma cell vs GC differentiation.
Is this relative positioning of Bmems in relation to the SCS niche important? Boosting of the dLN led to bigger and better Ab responses with more GCs & affinity maturation compared ndLN. But when SSMs are depleted with anti-CSF1R this effect is abolished.
April 29, 2025 at 1:20 PM
Is this relative positioning of Bmems in relation to the SCS niche important? Boosting of the dLN led to bigger and better Ab responses with more GCs & affinity maturation compared ndLN. But when SSMs are depleted with anti-CSF1R this effect is abolished.
Rama used intravital microscopy to show that Bmems were positioned deeper in follicle ndLN vs dLN where they were largely confined to the SCS layer. Depletion of CD169+ SCS macrophages (SSMs) with blocking anti-CSF1R mAb led to relocalisation of dLN Bmems deeper in the follicle.
April 29, 2025 at 1:20 PM
Rama used intravital microscopy to show that Bmems were positioned deeper in follicle ndLN vs dLN where they were largely confined to the SCS layer. Depletion of CD169+ SCS macrophages (SSMs) with blocking anti-CSF1R mAb led to relocalisation of dLN Bmems deeper in the follicle.
Look on my works, ye Mighty, and despair!
December 23, 2024 at 9:33 AM
Look on my works, ye Mighty, and despair!
B CELL FUN FACT: Secrets of the GC #1
Walther Flemming described GCs in 1885 paper "Studies in Tissue Regeneration". He mistakenly thought they were sites of cell birth (hence name) & tingible bodies ("tingiblen Körper") were daughter cells about to be born!
link.springer.com/article/10.1...
Walther Flemming described GCs in 1885 paper "Studies in Tissue Regeneration". He mistakenly thought they were sites of cell birth (hence name) & tingible bodies ("tingiblen Körper") were daughter cells about to be born!
link.springer.com/article/10.1...
December 6, 2024 at 12:02 AM
B CELL FUN FACT: Secrets of the GC #1
Walther Flemming described GCs in 1885 paper "Studies in Tissue Regeneration". He mistakenly thought they were sites of cell birth (hence name) & tingible bodies ("tingiblen Körper") were daughter cells about to be born!
link.springer.com/article/10.1...
Walther Flemming described GCs in 1885 paper "Studies in Tissue Regeneration". He mistakenly thought they were sites of cell birth (hence name) & tingible bodies ("tingiblen Körper") were daughter cells about to be born!
link.springer.com/article/10.1...
... presidential address by "the Austin Powers of Australian immunology" José Villadangos & awards ceremony was great reminder of all things wonderful ASI does for members & esp promoting ECRs & a fair go for everyone. Congrats to organisers on great meeting & all the winners! Proud to be a part! 🙏
December 2, 2024 at 8:39 PM
... presidential address by "the Austin Powers of Australian immunology" José Villadangos & awards ceremony was great reminder of all things wonderful ASI does for members & esp promoting ECRs & a fair go for everyone. Congrats to organisers on great meeting & all the winners! Proud to be a part! 🙏
This brings us to the last day #ASI2024 & a packed auditorium still bringing the energy! Laura Mackay kept it real talking about TRM cells & breast cancer. Kenji Kabashima talking about perivascular immune hubs in the skin. Claudier Kemper took us on a TdF through complosome - who knew!...
December 2, 2024 at 8:30 PM
This brings us to the last day #ASI2024 & a packed auditorium still bringing the energy! Laura Mackay kept it real talking about TRM cells & breast cancer. Kenji Kabashima talking about perivascular immune hubs in the skin. Claudier Kemper took us on a TdF through complosome - who knew!...
... #ASI2024 would not be complete without the conference dinner limerick competition won by Jarrod Kennedy & table 27:
For Ideas grants Suzanne waited,
But the news left her deflated,
If you've no Ideas,
Then have a few beers,
Next round just call yourself David!
But can you spot the bursa?
For Ideas grants Suzanne waited,
But the news left her deflated,
If you've no Ideas,
Then have a few beers,
Next round just call yourself David!
But can you spot the bursa?
December 1, 2024 at 11:49 AM
... #ASI2024 would not be complete without the conference dinner limerick competition won by Jarrod Kennedy & table 27:
For Ideas grants Suzanne waited,
But the news left her deflated,
If you've no Ideas,
Then have a few beers,
Next round just call yourself David!
But can you spot the bursa?
For Ideas grants Suzanne waited,
But the news left her deflated,
If you've no Ideas,
Then have a few beers,
Next round just call yourself David!
But can you spot the bursa?
...leading to THE highlight of #ASI2024 the Lafferty Debate chaired by Misty Jenkins featuring for AFF. Alex Davenport (C), Di Yu & Deborah Burnett & NEG. Antje Blumenthal (C), Vanessa Bryant & @ianparish.bsky.social with brilliant ROFL monologue about coffee...
Photo by @kimljacobson.bsky.social
Photo by @kimljacobson.bsky.social
December 1, 2024 at 2:21 AM
...leading to THE highlight of #ASI2024 the Lafferty Debate chaired by Misty Jenkins featuring for AFF. Alex Davenport (C), Di Yu & Deborah Burnett & NEG. Antje Blumenthal (C), Vanessa Bryant & @ianparish.bsky.social with brilliant ROFL monologue about coffee...
Photo by @kimljacobson.bsky.social
Photo by @kimljacobson.bsky.social
#ASI2024 day 3 was a big day for B cells! Julie Zikherman showed how particulate Ag bypass LYN inhibition, @montserrat-anguera.bsky.social showed how XCI in T cells contribute to SLE, there was a whole B cell session featuring @kimljacobson.bsky.social @iancockburn.bsky.social & Julia Ellyard...
December 1, 2024 at 1:53 AM
#ASI2024 day 3 was a big day for B cells! Julie Zikherman showed how particulate Ag bypass LYN inhibition, @montserrat-anguera.bsky.social showed how XCI in T cells contribute to SLE, there was a whole B cell session featuring @kimljacobson.bsky.social @iancockburn.bsky.social & Julia Ellyard...
It's been a whirlwind #ASI2024 only now catching my breath! Filling in the gaps...
Day 2 highlights Peter Doherty Medals awarded by Peter Doherty to Susan Christo & Carolien van de Sandt from the Peter Doherty Institute! Bookended by extraordinary Burnett Oration by the one & only Carola Vinuesa.
Day 2 highlights Peter Doherty Medals awarded by Peter Doherty to Susan Christo & Carolien van de Sandt from the Peter Doherty Institute! Bookended by extraordinary Burnett Oration by the one & only Carola Vinuesa.
November 30, 2024 at 3:03 AM
It's been a whirlwind #ASI2024 only now catching my breath! Filling in the gaps...
Day 2 highlights Peter Doherty Medals awarded by Peter Doherty to Susan Christo & Carolien van de Sandt from the Peter Doherty Institute! Bookended by extraordinary Burnett Oration by the one & only Carola Vinuesa.
Day 2 highlights Peter Doherty Medals awarded by Peter Doherty to Susan Christo & Carolien van de Sandt from the Peter Doherty Institute! Bookended by extraordinary Burnett Oration by the one & only Carola Vinuesa.
What an epic day 1 #ASI2024! So many brilliant talks. Highlight was Prof Antony Basten’s Foundation Lecture on the origin story of the “Australian model” of double fellowship in clinical & laboratory immunology. Did you know Jacques Miller, Gus Nossal and Don Metcalfe all trained at RPAH lab?
November 26, 2024 at 2:18 PM
What an epic day 1 #ASI2024! So many brilliant talks. Highlight was Prof Antony Basten’s Foundation Lecture on the origin story of the “Australian model” of double fellowship in clinical & laboratory immunology. Did you know Jacques Miller, Gus Nossal and Don Metcalfe all trained at RPAH lab?
The #ASI2024 SIG Workshops did not disappoint. Great range talks. Some highlights. Carola Vinuesa taking us on a TdF through TLR7 and monogenic SLE. Marcel Nold on his Anakinra Pilot study (comes with light sabre!) and @lggray95.bsky.social looking at XCI in SLE. Day 1 about officially start!
November 25, 2024 at 7:58 PM
The #ASI2024 SIG Workshops did not disappoint. Great range talks. Some highlights. Carola Vinuesa taking us on a TdF through TLR7 and monogenic SLE. Marcel Nold on his Anakinra Pilot study (comes with light sabre!) and @lggray95.bsky.social looking at XCI in SLE. Day 1 about officially start!
Tmrw is the start of #ASI2024 at the ICC in #Sydney. If you want to talk B cells | macrophages | germinal centres | vaccine-induced immunity | autoimmunity | SLE | all things immunology or if you are new to the Emerald City and just want tips on where to go pls mssg me!
November 24, 2024 at 12:17 PM