From functional studies: we found that Zbtb16 up-regulation due to delayed Bcl11b activation was necessary and sufficient for ILC2 redirection:

[Note that after up-regulating Zbtb16, cells can still fully turn on Bcl11b, which is also important for the ILC2 specification] (7/n)

How does ILC redirection happen? Using scRNA-seq, we found that delayed Bcl11b activation in progenitors results in up-regulation of the ILC regulator Zbtb16. (6/n)

Delaying Bcl11b activation moderately attenuated T cell output but, remarkably, redirected progenitors towards the type 2 innate lymphoid cells ILC2 lineages in thymus. (5/n)

We are curious how progenitors can give rise to multiple cell types in defined numbers and proportions. Unlike some embryos, blood progenitors often have to generate multiple cell types in the absence of predefined spatial cues. (see doi.org/10.1016/j.co...) (2/n)