Because this protocol retains proteins in the gel, it is natively compatible with antibody labeling, allowing localization of synaptic proteins, cell-type and subcellular markers with ultrastructural resolution, effectively a sort of “multicolor electron microscopy”. (3/5)

We developed a "pan-expansion" protocol that works well in brain tissue, using a ~20x iterative expansion compatible with pan-protein and lipid stains to reveal dense, nanoscale ultrastructure of neuronal circuits (~20 nm effective resolution) with a confocal microscope. (2/5)

Our new paper showcasing molecular connectomics with pan-expansion microscopy is out in @natbiotech.nature.com! www.nature.com/articles/s41...
This wonderful collaboration with @bewersdorflab.bsky.social was led by Ons M'Saad (now founder/CEO of Panluminate) and @allisonphysics.bsky.social. (1/5)