From sequencing results' analysis, we showed that 1) our method was a valuable tool for precise trait mapping - our locus under selection could be detected with ~ kilo base precision and 2) exposed a surprisingly diversity in recombined fragment sizes contrary to precedent data on conjugation. 4/4

We successfully created libraries of donors and mixed them with several recipient bacteria (from diverse strains and phylogroups). We obtained libraries of recombinants that we analyzed at the individual and population level through sequencing 🧵 3/4

In this paper, we developed a method for the construction of donor libraries combining the power of transposon mutagenesis & lambda-red Cas9 mediated recombination🧵 2/4