@jihwanjameslee.bsky.social
4/7
🎉 The result? Meet mGold2s and mGold2t:
🟡 ~20–25× more photostable than mVenus and mCitrine
🟡 4× more photostable than our previous champion, mGold
🟡 Retain high brightness and other properties
🎉 The result? Meet mGold2s and mGold2t:
🟡 ~20–25× more photostable than mVenus and mCitrine
🟡 4× more photostable than our previous champion, mGold
🟡 Retain high brightness and other properties
April 9, 2025 at 8:59 PM
4/7
🎉 The result? Meet mGold2s and mGold2t:
🟡 ~20–25× more photostable than mVenus and mCitrine
🟡 4× more photostable than our previous champion, mGold
🟡 Retain high brightness and other properties
🎉 The result? Meet mGold2s and mGold2t:
🟡 ~20–25× more photostable than mVenus and mCitrine
🟡 4× more photostable than our previous champion, mGold
🟡 Retain high brightness and other properties
3/7
To fix this, we used SPOTlight—our pooled, single-cell, microscopy-based screening platform—to screen 1.1 million cells encoding >200,000 variants.
By simultaneously screening for brightness and photostability, we beat the typical trade-off between the two.
To fix this, we used SPOTlight—our pooled, single-cell, microscopy-based screening platform—to screen 1.1 million cells encoding >200,000 variants.
By simultaneously screening for brightness and photostability, we beat the typical trade-off between the two.
April 9, 2025 at 8:59 PM
3/7
To fix this, we used SPOTlight—our pooled, single-cell, microscopy-based screening platform—to screen 1.1 million cells encoding >200,000 variants.
By simultaneously screening for brightness and photostability, we beat the typical trade-off between the two.
To fix this, we used SPOTlight—our pooled, single-cell, microscopy-based screening platform—to screen 1.1 million cells encoding >200,000 variants.
By simultaneously screening for brightness and photostability, we beat the typical trade-off between the two.
🧵 1/6
Just dropped in @naturecomms.bsky.social
We’ve engineered the most photostable yellow fluorescent proteins (YFPs) to date—mGold2s and mGold2t—with up to 25× greater photostability than mVenus and mCitrine, without compromising brightness.
👇
🔗 www.nature.com/articles/s41...
Just dropped in @naturecomms.bsky.social
We’ve engineered the most photostable yellow fluorescent proteins (YFPs) to date—mGold2s and mGold2t—with up to 25× greater photostability than mVenus and mCitrine, without compromising brightness.
👇
🔗 www.nature.com/articles/s41...
April 9, 2025 at 8:59 PM
🧵 1/6
Just dropped in @naturecomms.bsky.social
We’ve engineered the most photostable yellow fluorescent proteins (YFPs) to date—mGold2s and mGold2t—with up to 25× greater photostability than mVenus and mCitrine, without compromising brightness.
👇
🔗 www.nature.com/articles/s41...
Just dropped in @naturecomms.bsky.social
We’ve engineered the most photostable yellow fluorescent proteins (YFPs) to date—mGold2s and mGold2t—with up to 25× greater photostability than mVenus and mCitrine, without compromising brightness.
👇
🔗 www.nature.com/articles/s41...