Jan Felix
@janfelix.bsky.social
Structural Biologist from Gent. Former Post-Doc @ Gutsche group, IBS, Grenoble, FR and @ Savvides Lab, IRC, Gent, BE.
Currently part of the VIB Bioimaging Core Gent Team.
Interested in ❄️🔬 #CryoEM and #Cytokines.
Currently part of the VIB Bioimaging Core Gent Team.
Interested in ❄️🔬 #CryoEM and #Cytokines.
Just tried your HR-HAIR + local refinement pipeline (with tweaked settings based on the pre-print) on a dataset of a small protein complex which did not result in any decent ab-initio reconstructions. HR-HAIR took 19 hours, but damn, result is insane! Many thanks!
September 17, 2025 at 7:39 AM
Just tried your HR-HAIR + local refinement pipeline (with tweaked settings based on the pre-print) on a dataset of a small protein complex which did not result in any decent ab-initio reconstructions. HR-HAIR took 19 hours, but damn, result is insane! Many thanks!
This is fantastic!! Very nice work!
September 15, 2025 at 7:19 AM
This is fantastic!! Very nice work!
And additionally, many thanks to Wouter Van Putte @pxn001.bsky.social (PUXANO) for providing their fantastic graphene-coated EM grids!!!
September 2, 2025 at 11:49 AM
And additionally, many thanks to Wouter Van Putte @pxn001.bsky.social (PUXANO) for providing their fantastic graphene-coated EM grids!!!
Congrats to everyone involved at Sophie Lucas Lab (especially Blueskyless co-first authors Fanny Lambert and Séverine Wautier), @argenx.com and @savvideslab.bsky.social at @viblifesciences.bsky.social!
September 2, 2025 at 11:41 AM
Congrats to everyone involved at Sophie Lucas Lab (especially Blueskyless co-first authors Fanny Lambert and Séverine Wautier), @argenx.com and @savvideslab.bsky.social at @viblifesciences.bsky.social!
It is definitely a pseudo symmetry case (symmetric dimeric receptor assembly with asymmetric ligand in between). I did not try your suggested workflow. For this particular case 3D orientation rebalancing + BLUSH combo did the trick and results in a nice 3ish Å map from severely anisotropic data.
January 10, 2025 at 4:04 PM
It is definitely a pseudo symmetry case (symmetric dimeric receptor assembly with asymmetric ligand in between). I did not try your suggested workflow. For this particular case 3D orientation rebalancing + BLUSH combo did the trick and results in a nice 3ish Å map from severely anisotropic data.