Joey Tidei
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j-today.bsky.social
Joey Tidei
@j-today.bsky.social
PhD candidate in the Beach and Oakes Labs at Loyola University Chicago. I like microscopes. I like neurons. I like bread.
Microtubules 🟢 protruding into the dynamic actin 🟣 network of a neuronal growth cone in DIV2 hippocampal neurons. @spirochrome.com SiR-Tubulin and SPY-FastActX working wonders on this #FluorescenceFriday
August 1, 2025 at 12:47 PM
Reposted by Joey Tidei
Our new preprint is out! Led by @stefanosala89.bsky.social sky.social we investigate the difference between molecular tension sensing and global tension on the focal adhesion. Follow along for a thread of our findings:
www.biorxiv.org/content/10.1...
LIM Domain Proteins link molecular and global tension by recognizing strained actin in adhesions
Mechanotransduction is fundamental to cell signaling and depends on force-sensitive adhesion proteins. How these proteins differentiate and integrate their responses to tension remains an open questio...
www.biorxiv.org
July 21, 2025 at 5:33 PM
Reposted by Joey Tidei
A day ahead of #FluorescenceFriday, a (temporal) switch from the bulb to the cortex.

🟣 NeuN
🔵 AnkG

🔬🧠🧪
July 10, 2025 at 9:16 AM
Imaging neurons quickly for 3min typically won't give you huge cell-wide cytoskeletal events, but you do get to capture fun little tugs and loops in the soma and neurites (as well as whatever the hell is going on in the bottom right).

Stain: @spirochrome.com SiR-Tubulin

Happy #FlourescenceFriday!
May 9, 2025 at 1:40 PM
Did you wake up this morning and think "Huh, I'd love to learn more about how myosin 2 filaments assemble and their contribution to cell architecture and function"? What a coincidence, I've got just the review for you.
Myosin 2 – A general contractor for the cytoskeleton
Cells derive their shape, and in turn much of their behavior, from the organization of the cytoskeleton. While a myriad of proteins contribute to the …
www.sciencedirect.com
May 5, 2025 at 3:39 PM
Reposted by Joey Tidei
Our PhD student Louis Romette is optimizing long-term live-cell imaging. He just casually dropped this 65-hour movie of a growing neuron (div 3 to 6) with knocked-in actin 🔥🕶️ #realtimemicroscopy
April 17, 2025 at 1:30 PM
Reposted by Joey Tidei
Two months left for FASEB Conference - Septins: Spatial regulators of cell biology
Register now:
events.faseb.org/event/septin...
Come find out what these filaments do at the interface of the actin and microtubule cytoskeletons and cell membranes. You'll be hooked for life.
April 16, 2025 at 3:08 PM
Reposted by Joey Tidei
Congrats to the NeuroArt Grand Prize winners!
🥇 Totally Tubular –Joey Tidei @j-today.bsky.social
🥈 Neurosphere – Adam Soos
🥉 Unwelcome Guest – Danielle Beckman @daniellebeckman.bsky.social

Thanks to all who shared their art! Want to be next? Enter our contest! 🔗 neuroart.com/annual-grand...
March 18, 2025 at 5:53 PM
Reposted by Joey Tidei
For this #FluorescenceFriday I share another example of the possibilities of making art with imageJ processing. This is a 3 day long time-lapse of a growing slime mold, with 15 min growth waves color coded.
the white things are oat flakes. I'll share the original video in comments

#Sciart
March 14, 2025 at 8:05 PM
I've been lacking on #FluorescenceFriday the past month or two, but winning the annual MBF NeuroArt competition was a lovely surprise to end the week. Thank you @mbfbioscience.bsky.social for the recognition!

neuroart.com/annual-grand...
March 14, 2025 at 7:35 PM
Zoomed in on the base of an axon looking at some funky actin dynamics for this #FluorescenceFriday
January 17, 2025 at 1:30 PM
Reposted by Joey Tidei
#TIRFSIMThursday with a COS cell labeled for actin, clathrin and microtubules
January 2, 2025 at 8:18 PM
Reposted by Joey Tidei
It'll be a slightly smaller crew than previous years but we'll still be at #cellbio24 #ASCB! If you like LIM proteins or septins come check out our posters:
December 12, 2024 at 4:51 PM
This place really is bumpin’… My first #FluorescenceFriday on Bluesky. Recycled, but a DIV4 rat hippocampal neuron expressing a probe for F-Actin and imaged on an LSM880 with Airyscan.
November 22, 2024 at 2:26 PM
Reposted by Joey Tidei
Hi everyone! Kicking off my first #FluorescenceFriday here with a 3D SIM image of actin, and of course had to go with my favorite blue LUT (JDM Duo Intense Cyan) for Bluesky.
November 15, 2024 at 5:46 PM