Looking for a method to characterise cell #motility and #ERK signalling waves?
Check out our STAR protocols paper:
star-protocols.cell.com/protocols/41...

Thanks for the great work Julio Cesar Sanchez-Rendon & @effiebastounis.bsky.social!

#protocol #epithelia #FRET #singlecell #imaging

Collective infected cell extrusion (CICE) also occurs with another #pathogen & in vivo! Huge thanks to Marie Muenkel & the @sergemostowylab.bsky.social lab for showing that #zebrafish larvae fins infected with #Shigella also extrude the infected cells in an ERK activity dependent manner. (3/4)

Epithelial cells in monolayer ( #MDCK) coordinate their collective cell migration via ERK activation waves, thereby squeezing & extruding #host cells infected with #Listeria. ERK inhibition or constitutive activation abolish ERK activation waves and obstruct this cell extrusion. (2/4)