Thanks Ute!

Thanks very much Johana!

5/5. MetaFlowTrain confirms that specific bacterial exometabolites, not media depletion, drive microbial antagonism.
🦠 Pseudomonas R401 WT suppresses SynCom growth via known inhibitory exometabolites.
❌ A triple mutant loses inhibitory power.

4/5. MetaFlowTrain dissects inter-kingdom microbial interactions via directional exometabolite flow.
🦠 Bacterial SynComs strongly inhibit fungal growth, even without physical contact.
🚫 Up to 82% growth suppression via exometabolites.

3/5. MetaFlowTrain reveals how soil conditioning alters microbial assembly/exometabolite output.
🦠 SynComs exposed to plant-conditioned peat undergo major community shifts & consume key metabolites (α-KG, fumarate).
🌱 BF SynCom-derived exometabolites impact Arabidopsis germination & root growth.

2/5. MetaFlowTrain cultivates diverse microbes & collects their exometabolites under continuous nutrient flow.
🌱 Using sterile peat extract, we grew 11 bacterial & 4 fungal strains from the Arabidopsis root microbiota.
💥 Exometabolite profiles differed significantly by strain.

📝 Paper: www.nature.com/articles/s41...
1/5. 🚂 MetaFlowTrain = 4 modular components:
a. Variety of liquid inputs
b. 24-channel pump enabling parallel metabolic trains
c. 3D-printed gnotobiotic microchambers
d. Multi-layered outputs for cell/metabolite analysis
🔗 protocols.io/view/36wgqd68ovk5/v3

Hi Ken! Thanks!!!

Thanks! Let’s hope this system will be useful to the community.

🙏🙏🙏

Thanks! We will be happy to help you to set it up.

Many thanks Fantin!!!