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Upon PAM recognition the DNA-scanning supercomplex adopts the 'docked' conformation. Its further rearrangement via displacement of #Cas9 REC2 domain yields the 'locked' supercomplex with straight DNA.
June 13, 2025 at 10:48 AM
Upon PAM recognition the DNA-scanning supercomplex adopts the 'docked' conformation. Its further rearrangement via displacement of #Cas9 REC2 domain yields the 'locked' supercomplex with straight DNA.
Here, we present #cryoEM structures of the type II-A prespacer selection supercomplex consisting of #Cas9 RNP, Cas1-Cas2 #integrase fragment and Csn2 ring in the DNA-scanning and two different PAM-bound configurations.
June 13, 2025 at 10:48 AM
Here, we present #cryoEM structures of the type II-A prespacer selection supercomplex consisting of #Cas9 RNP, Cas1-Cas2 #integrase fragment and Csn2 ring in the DNA-scanning and two different PAM-bound configurations.