Giacomo Donati
@giacomodonatijd.bsky.social
Associate Prof. @ University of Turin | Into RNA, chromatin & stem cell adaptation in wound healing and early epithelial tumorigenesis | www.donatilab.org | Ex @cruk-ci.bsky.social @KCLstemcells
Reposted by Giacomo Donati
I am thrilled to share the latest manuscript from the Shain lab, describing the genetic changes that transform a keratinocyte into cutaneous squamous cell carcinoma (and everything in between). www.nature.com/articles/s41...
Genetic evolution of keratinocytes to cutaneous squamous cell carcinoma
Nature Communications - The evolution of cutaneous squamous cell carcinoma (cSCC) remains poorly understood. Here, the authors employ multi-omics and multi-scale analyses to explore the genetic...
www.nature.com
November 27, 2025 at 4:11 PM
I am thrilled to share the latest manuscript from the Shain lab, describing the genetic changes that transform a keratinocyte into cutaneous squamous cell carcinoma (and everything in between). www.nature.com/articles/s41...
Reposted by Giacomo Donati
The latest in my favorite love triangle:
Chromatin <-(💜)-> Condensates <-(💚)-> Mechanics
Chromatin <-(💜)-> Condensates <-(💚)-> Mechanics
Congrats to Jing @jesszhiyuezhao.bsky.social
& @dnamystrom.bsky.social on cool manuscript!!! Reveals how cell nucleus is a heterogeneous material, w chromatin organization controlling formation & phase behavior of nuclear condensates. #CellBiology #Chromatin #PhaseSeparation rdcu.be/evWxR
& @dnamystrom.bsky.social on cool manuscript!!! Reveals how cell nucleus is a heterogeneous material, w chromatin organization controlling formation & phase behavior of nuclear condensates. #CellBiology #Chromatin #PhaseSeparation rdcu.be/evWxR
Chromatin heterogeneity modulates nuclear condensate dynamics and phase behavior
Nature Communications - Chromatin heterogeneity in the cell nucleus modulates the size, mobility, and formation of biomolecular condensates; decreasing chromatin heterogeneity correlates with...
rdcu.be
July 13, 2025 at 4:46 PM
The latest in my favorite love triangle:
Chromatin <-(💜)-> Condensates <-(💚)-> Mechanics
Chromatin <-(💜)-> Condensates <-(💚)-> Mechanics
Reposted by Giacomo Donati
Selective RNA sequestration in biomolecular condensates directs cell fate transitions - @distefanolab.bsky.social @brumbaugh-lab.bsky.social go.nature.com/3X215du
Selective RNA sequestration in biomolecular condensates directs cell fate transitions - Nature Biotechnology
Stem cell differentiation is controlled by manipulating RNA condensates.
go.nature.com
October 28, 2025 at 6:50 PM
Selective RNA sequestration in biomolecular condensates directs cell fate transitions - @distefanolab.bsky.social @brumbaugh-lab.bsky.social go.nature.com/3X215du
Reposted by Giacomo Donati
Now online! Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunity
Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunity
Acute stress causes immediate hair follicle necrosis and hair loss via sympathetic nerves, which in turn activates and expands autoreactive CD8⁺ T cells targeting hair follicles. Thus, a single stress event not only causes immediate tissue damage but also predisposes the tissue to future autoimmune attack.
dlvr.it
November 26, 2025 at 4:00 PM
Now online! Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunity
Reposted by Giacomo Donati
Finally announcing our new study in @cp-immunity.bsky.social!
We show that IL-17RA fulfills dual tumor suppressor function in late-stage colorectal cancer (CRC)!
#immunosky #cancer #immunology @loewe-fci.bsky.social
@cellpress.bsky.social
@goetheuni.bsky.social
Read here🧵👇:
tinylink.net/OS41F
We show that IL-17RA fulfills dual tumor suppressor function in late-stage colorectal cancer (CRC)!
#immunosky #cancer #immunology @loewe-fci.bsky.social
@cellpress.bsky.social
@goetheuni.bsky.social
Read here🧵👇:
tinylink.net/OS41F
IL-17RA signaling provides dual tumor-suppressor function during late-stage colorectal carcinogenesis
Members of the interleukin-17 family are associated with tumor progression in various
settings. Denk et al. find that, in colorectal cancer, IL-17RA switches from tumor
promotion during early tumorige...
www.cell.com
February 28, 2025 at 7:49 PM
Finally announcing our new study in @cp-immunity.bsky.social!
We show that IL-17RA fulfills dual tumor suppressor function in late-stage colorectal cancer (CRC)!
#immunosky #cancer #immunology @loewe-fci.bsky.social
@cellpress.bsky.social
@goetheuni.bsky.social
Read here🧵👇:
tinylink.net/OS41F
We show that IL-17RA fulfills dual tumor suppressor function in late-stage colorectal cancer (CRC)!
#immunosky #cancer #immunology @loewe-fci.bsky.social
@cellpress.bsky.social
@goetheuni.bsky.social
Read here🧵👇:
tinylink.net/OS41F
Reposted by Giacomo Donati
Messenger RNA is made in the nucleus before it is exported to the cytoplasm for translation. But how are only correctly made mRNAs chosen and remodeled in the nucleus for export?
Our new paper investigates the nuclear events leading to human mRNA export. www.nature.com/articles/s41.... (1/4)
Our new paper investigates the nuclear events leading to human mRNA export. www.nature.com/articles/s41.... (1/4)
November 21, 2025 at 5:02 AM
Messenger RNA is made in the nucleus before it is exported to the cytoplasm for translation. But how are only correctly made mRNAs chosen and remodeled in the nucleus for export?
Our new paper investigates the nuclear events leading to human mRNA export. www.nature.com/articles/s41.... (1/4)
Our new paper investigates the nuclear events leading to human mRNA export. www.nature.com/articles/s41.... (1/4)
Reposted by Giacomo Donati
Last but not least, we are thrilled to have representatives from academia, industry, foundations and publishers to share with us their take during the roundtable discussion on Bridging Sectors for Translational Impact!
November 20, 2025 at 4:50 PM
Last but not least, we are thrilled to have representatives from academia, industry, foundations and publishers to share with us their take during the roundtable discussion on Bridging Sectors for Translational Impact!
Reposted by Giacomo Donati
The 2nd @eurocancerdepmap.bsky.social meeting is starting with introduction from @francescoiorio.bsky.social ! Full day of exciting talks ahead. Stay with us for live updates @humantechnopole.bsky.social
November 20, 2025 at 8:59 AM
The 2nd @eurocancerdepmap.bsky.social meeting is starting with introduction from @francescoiorio.bsky.social ! Full day of exciting talks ahead. Stay with us for live updates @humantechnopole.bsky.social
Reposted by Giacomo Donati
Our latest paper has just been published in Cell!
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
November 5, 2025 at 5:20 PM
Our latest paper has just been published in Cell!
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
Reposted by Giacomo Donati
Great collaboration on uncovering how ER organisation, curvature and mechanics are regulating collective cell migration. Experiments by Simran, Tamal and collaborators and a model by Pradeep.
💫NEW: Das, @fabianspill.bsky.social & co combine intracellular cartography and biophysical modelling to reveal how edge curvature governs endoplasmic reticulum (ER) morphology, showing that curvature-dependent ER reorganization directs distinct modes of epithelial cell migration.
bit.ly/3HFAQ8S
bit.ly/3HFAQ8S
Edge curvature drives endoplasmic reticulum reorganization and dictates epithelial migration mode - Nature Cell Biology
Rawal et al. combine intracellular cartography and biophysical modelling to reveal how edge curvature governs endoplasmic reticulum (ER) morphology, showing that curvature-dependent ER reorganization ...
bit.ly
September 8, 2025 at 7:40 PM
Great collaboration on uncovering how ER organisation, curvature and mechanics are regulating collective cell migration. Experiments by Simran, Tamal and collaborators and a model by Pradeep.
Reposted by Giacomo Donati
Regulatory T cells in skin utilize the Cxcr4-Cxcl12 axis to promote hair follicle regeneration @cp-cellreports.bsky.social
www.cell.com/cell-reports...
www.cell.com/cell-reports...
October 23, 2025 at 5:07 PM
Regulatory T cells in skin utilize the Cxcr4-Cxcl12 axis to promote hair follicle regeneration @cp-cellreports.bsky.social
www.cell.com/cell-reports...
www.cell.com/cell-reports...
Reposted by Giacomo Donati
Multimodal learning enables chat-based exploration of single-cell data - @bocklab.bsky.social @cemm.oeaw.ac.at @meduniwien.ac.at go.nature.com/3WPGJnW
Multimodal learning enables chat-based exploration of single-cell data - Nature Biotechnology
CellWhisperer uses multimodal learning of transcriptomes and text to answer questions about single-cell RNA-sequencing data.
go.nature.com
November 11, 2025 at 4:58 PM
Multimodal learning enables chat-based exploration of single-cell data - @bocklab.bsky.social @cemm.oeaw.ac.at @meduniwien.ac.at go.nature.com/3WPGJnW
Reposted by Giacomo Donati
How does aging alter the translational landscape of stem cells and how do these changes impact their regenerative capacity? Here we developed an in vivo single-cell ribosome profiling strategy to monitor translational landscapes of the mouse epidermis 1/6
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 18, 2024 at 3:35 PM
How does aging alter the translational landscape of stem cells and how do these changes impact their regenerative capacity? Here we developed an in vivo single-cell ribosome profiling strategy to monitor translational landscapes of the mouse epidermis 1/6
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Giacomo Donati
Fibroinflammatory skin diseases are characterized by interferon independent fibroblast EGFR-STAT1 signaling that controls expression of fibrotic genes
@natcomms.nature.com @yalepress.bsky.social
www.nature.com/articles/s41...
@natcomms.nature.com @yalepress.bsky.social
www.nature.com/articles/s41...
October 10, 2025 at 2:23 AM
Fibroinflammatory skin diseases are characterized by interferon independent fibroblast EGFR-STAT1 signaling that controls expression of fibrotic genes
@natcomms.nature.com @yalepress.bsky.social
www.nature.com/articles/s41...
@natcomms.nature.com @yalepress.bsky.social
www.nature.com/articles/s41...
Reposted by Giacomo Donati
My recent postdoctoral work is out today in @nature.com! We found that physical confinement makes melanoma cells more invasive and drug tolerant. Thanks to all who made this work possible @whitefishlab.bsky.social @mskcancercenter.bsky.social #NIH #NCI www.nature.com/articles/s41...
Mechanical confinement governs phenotypic plasticity in melanoma - Nature
Mechanical confinement of cancer cells at the tumour–microenvironment interface induces phenotype switching through chromatin remodelling by HMGB2, leading to a more invasive and drug-resistant state ...
www.nature.com
August 27, 2025 at 5:18 PM
My recent postdoctoral work is out today in @nature.com! We found that physical confinement makes melanoma cells more invasive and drug tolerant. Thanks to all who made this work possible @whitefishlab.bsky.social @mskcancercenter.bsky.social #NIH #NCI www.nature.com/articles/s41...
Reposted by Giacomo Donati
Latest from @ivaskalab.bsky.social @jrwconway.bsky.social in NCB. We find that SHP-2 inhibition stops TNBC invasion and limits metastasis by blocking integrin dephosphorylation!
Read more at:
www.nature.com/articles/s41...
Read more at:
www.nature.com/articles/s41...
Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells - Nature Cell Biology
Conway et al. show that integrin β1 phosphorylation is regulated through balanced activities of tyrosine kinases, such as Src and Arg, and phosphatases, such as PTP-PEST and Shp2, facilitating invadop...
www.nature.com
May 26, 2025 at 12:16 PM
Latest from @ivaskalab.bsky.social @jrwconway.bsky.social in NCB. We find that SHP-2 inhibition stops TNBC invasion and limits metastasis by blocking integrin dephosphorylation!
Read more at:
www.nature.com/articles/s41...
Read more at:
www.nature.com/articles/s41...
Clinically-normal skin harbours widespread, sub-clinical fields of BRAF V600E-mutant melanocytes, which are oncogene-primed but apparently dormant. These fields concentrated around naevi & melanomas may represent a field cancerization. @thestarklab.bsky.social url: academic.oup.com/bjd/article/...
Subclinical Fields of BRAF V600E-Mutant Melanocytes Populate Human Skin and Are Enriched Around Melanoma and Naevi
The origins of cutaneous melanoma are often traced to visible precursor lesions (e.g., melanocytic naevi), but ∼2/3 melanomas arise from clinically normal
academic.oup.com
October 30, 2025 at 10:33 PM
Clinically-normal skin harbours widespread, sub-clinical fields of BRAF V600E-mutant melanocytes, which are oncogene-primed but apparently dormant. These fields concentrated around naevi & melanomas may represent a field cancerization. @thestarklab.bsky.social url: academic.oup.com/bjd/article/...
Reposted by Giacomo Donati
Programmable promoter editing for precise control of transgene expression - @mitcheme.bsky.social @mitdeptofbe.bsky.social go.nature.com/4h9wlR1
October 13, 2025 at 7:11 PM
Programmable promoter editing for precise control of transgene expression - @mitcheme.bsky.social @mitdeptofbe.bsky.social go.nature.com/4h9wlR1
Reposted by Giacomo Donati
Pleased to share our review on “Synthetic Mechanotransduction” — now live at Nature Reviews Bioengineering.
May the force be with you!
www.nature.com/articles/s44...
May the force be with you!
www.nature.com/articles/s44...
Synthetic mechanotransduction - Nature Reviews Bioengineering
Cells convert mechanical forces into biochemical signals through mechanotransduction, a process which can now be synthetically engineered. In this Review, we examine engineered artificial mechanotrans...
www.nature.com
October 14, 2025 at 5:03 PM
Pleased to share our review on “Synthetic Mechanotransduction” — now live at Nature Reviews Bioengineering.
May the force be with you!
www.nature.com/articles/s44...
May the force be with you!
www.nature.com/articles/s44...
Reposted by Giacomo Donati
We found that the ECM composition underlying the stem cells affects wound healing speed!
October 10, 2025 at 3:42 PM
We found that the ECM composition underlying the stem cells affects wound healing speed!
Reposted by Giacomo Donati
LOV-BirA, light regulated biotin ligase, engineered by Song-Yi Lee
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
August 27, 2025 at 10:22 PM
Reposted by Giacomo Donati
Check out our new RAEFISH spatial transcriptomics technology published in Cell! This work represents the first time that transcripts from more than 20,000 genes were directly imaged in situ with any technology, and the first time numerous gRNAs were directly probed in an image-based CRISPR screen.
Now online! Sequencing-free whole-genome spatial transcriptomics at single-molecule resolution
Sequencing-free whole-genome spatial transcriptomics at single-molecule resolution
RAEFISH delivers whole-transcriptome (>20,000 genes) imaging at single-molecule resolution in cells and tissues and enables direct gRNA detection for high-content, image-based CRISPR screens.
dlvr.it
October 8, 2025 at 7:24 AM
Check out our new RAEFISH spatial transcriptomics technology published in Cell! This work represents the first time that transcripts from more than 20,000 genes were directly imaged in situ with any technology, and the first time numerous gRNAs were directly probed in an image-based CRISPR screen.
Reposted by Giacomo Donati
What would happen if cells would express more molecular motors ?
They would break all microtubules 😱
As shown by recent great work from @kjverhey1.bsky.social
rupress.org/jcb/article-...
They would break all microtubules 😱
As shown by recent great work from @kjverhey1.bsky.social
rupress.org/jcb/article-...
October 7, 2025 at 3:08 PM
What would happen if cells would express more molecular motors ?
They would break all microtubules 😱
As shown by recent great work from @kjverhey1.bsky.social
rupress.org/jcb/article-...
They would break all microtubules 😱
As shown by recent great work from @kjverhey1.bsky.social
rupress.org/jcb/article-...
Reposted by Giacomo Donati
How do #stemcells integrate information to coordinate fate decisions? Delighted to finally see our work showing how growth factors regulate the mechano-osmotic state of the #nucleus and #chromatin to control #pluripotency exit out! www.nature.com/articles/s41... see 🧵 👇
Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells - Nature Cell Biology
McCreery, Stubb et al. show that mechano-osmotic changes in the nucleus induce general transcriptional repression and prime chromatin for cell fate transitions by relieving repression of specific differentiation genes.
www.nature.com
September 29, 2025 at 4:45 PM
How do #stemcells integrate information to coordinate fate decisions? Delighted to finally see our work showing how growth factors regulate the mechano-osmotic state of the #nucleus and #chromatin to control #pluripotency exit out! www.nature.com/articles/s41... see 🧵 👇