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We’re entering an exciting era of gene-scale chromatin reconstitution with precise modification patterns🚀 We hope this work will stimulate further studies to understand how different histone modifications and solution environment control chromatin architecture and function. 5/5

We developed an in vitro Hi-C method to map nucleosome interactions and found how the acetylation reduces the nucleosome interaction frequency. For the ligated arrays (12-96-mer mixture), we found that the acetylation patterns directly control the long-range contact patterns. 4/5

We observed arrays with different H4 hyperacetylation patterns, and found that higher acetylation density makes the array larger. There's an interesting relationship between the size and fluctuation timescales which agrees with the prediction of a simple polymer model. 3/5

How do histone modifications affect large-scale chromatin structure? We tackled this question in vitro by reconstituting gene-scale chromatin arrays with precise histone modification patterns and analyzing them using single-molecule fluorescence imaging and in vitro Hi-C. 2/5