couldn't see the end of it 😍

beautiful day to support science in NYC 💪🏻 #standupforscience

Very proud of my lab members (past and present) for receiving the Blue Flame Award from
@addgene.bsky.social based on some of our most requested and distributed plasmid reagents 😀:
www.addgene.org/Franck_Polle...

Addgene is such a great resource for the scientific community!

Surprisingly, our work uncovered compartment-specific differences in the magnitude and temporal expression of synaptic plasticity between basal and oblique dendrites. 15/n

Our key results (Fig. 4&5) identified a temporally asymmetric synaptic plasticity kernel resulting from bidirectional modifications of synaptic weights around the induction of a place field. 14/n

(2) To measure changes in functional synaptic strength in vivo before and after PF induction, we used a red-shifted calcium indicator (jRGECO1a) to monitor spine calcium dynamics. We demonstrate, using a new GEVI (ASAP6) from Michael Z. Lin that spine Ca2+ amplitude faithfully reports EPSP (ΔW) 13/n

To investigate the relationship between changes in synaptic weights (ΔW) and the emergence of PFs in individual CA1PNs, we developed and deployed three tools at single-cell resolution (Fig. 1): 11/n

How can a neuron receiving 10,000-15,000 synaptic inputs each providing broad information (tuning) ultimately decide to fire in a highly selective manner i.e. becoming feature selective? 3/n