Fran Bottanelli
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franbottanelli.bsky.social
Fran Bottanelli
@franbottanelli.bsky.social
Solver of GTPases molecular puzzles | Membranes | CRISPR | STED super-res |Accidental immunologist | Academic mother of 2 cheeky monkeys | Assistant prof at FU Berlin. | Head of the Nanoscale Membrane Dynamics Lab
I agree I mean next thing is pineapple on Spätzle??
November 11, 2025 at 5:55 PM
But we have also used a TfR fusion. That also works great.
November 11, 2025 at 3:20 PM
We have Rab11 plasmids if you need. Not as clean as endogenous but we see no weird things. We have used both and yes simply feeding fluorescent transferrin to cells work great.
November 11, 2025 at 3:19 PM
We have homozygous Rab11 KI which seems to be fine and kinetics of Tf are unchanged. That said you have different flavors of REs :) Rab4 labels EE and RE but we were reported overexpression is problematic and one should be careful.TfR will also label EE but also all flavors of RE nicely.
November 11, 2025 at 2:35 PM
Hi Mara 😊 you mean which protein to tag?
November 11, 2025 at 2:20 PM
Original music or covers? Ahahah for covers I may have to cover some Taylor Swift for the lab ahahh
November 6, 2025 at 11:19 AM
Reposted by Fran Bottanelli
Join @felixmendu.bsky.social, @franbottanelli.bsky.social, and me for this edition of #MembraneTrafficking seminars for this month's discussions on the molecular mechanisms underlying membrane biology.
More info: felixcampelo.wixsite.com/membranetraf...
Slack channel: join.slack.com/t/membranetr...
MEMBRANE TRAFFICKING | Membrane Trafficking
felixcampelo.wixsite.com
November 3, 2025 at 7:33 PM
My point is that academia cannot be like that. It cannot be the place where you have to keep going or hang in there with no prospect and safety net.
November 3, 2025 at 4:45 PM
Reposted by Fran Bottanelli
As usual, more info on the website (which will hopefully be updated soon :)
felixcampelo.wixsite.com/membranetraf...
cc/ @ishier.bsky.social @franbottanelli.bsky.social
MEMBRANE TRAFFICKING | Membrane Trafficking
felixcampelo.wixsite.com
October 27, 2025 at 5:02 PM