I agree I mean next thing is pineapple on Spätzle??

But we have also used a TfR fusion. That also works great.

We have Rab11 plasmids if you need. Not as clean as endogenous but we see no weird things. We have used both and yes simply feeding fluorescent transferrin to cells work great.

We have homozygous Rab11 KI which seems to be fine and kinetics of Tf are unchanged. That said you have different flavors of REs :) Rab4 labels EE and RE but we were reported overexpression is problematic and one should be careful.TfR will also label EE but also all flavors of RE nicely.

Hi Mara 😊 you mean which protein to tag?

Original music or covers? Ahahah for covers I may have to cover some Taylor Swift for the lab ahahh

Join @felixmendu.bsky.social, @franbottanelli.bsky.social, and me for this edition of #MembraneTrafficking seminars for this month's discussions on the molecular mechanisms underlying membrane biology.
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My point is that academia cannot be like that. It cannot be the place where you have to keep going or hang in there with no prospect and safety net.

As usual, more info on the website (which will hopefully be updated soon :)
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