Geoff Faulkner
@faulknerlab.bsky.social
Professor, University of Queensland. Retrotransposons and genomics.
Views my own. Scholar publications: http://tinyurl.com/3k7r3xvj
Views my own. Scholar publications: http://tinyurl.com/3k7r3xvj
The source rover ERV locus reminds me a lot of the human source L1 we found in Fig. 6 of PMID: 31230816 that had jumped in brain. Makes me think a lot of somatic mobility in eukaryotes may come from source 'escapee' elements that happen to land next to an active enhancer or promoter. 2/3
February 10, 2025 at 3:31 AM
The source rover ERV locus reminds me a lot of the human source L1 we found in Fig. 6 of PMID: 31230816 that had jumped in brain. Makes me think a lot of somatic mobility in eukaryotes may come from source 'escapee' elements that happen to land next to an active enhancer or promoter. 2/3
Had an excellent few days at the Frontiers in Epigenetics meeting in Kumamoto, hosted by Kazuya Iwamoto and Miki Bundo. Great science and company. Even got in a couple of runs around the city ... autumn seems to be late!
November 22, 2024 at 10:10 PM
Had an excellent few days at the Frontiers in Epigenetics meeting in Kumamoto, hosted by Kazuya Iwamoto and Miki Bundo. Great science and company. Even got in a couple of runs around the city ... autumn seems to be late!
4. We tested the effects of expressing the reported Caps2.L1 fusion transcript in cultured mouse neuroblastoma (N2a) cells, surprisingly finding that this transcript greatly increased neuron morphological complexity, whilst the canonical Caps2 transcript did not.
January 30, 2024 at 10:59 AM
4. We tested the effects of expressing the reported Caps2.L1 fusion transcript in cultured mouse neuroblastoma (N2a) cells, surprisingly finding that this transcript greatly increased neuron morphological complexity, whilst the canonical Caps2 transcript did not.
3. Development of a human L1 5'UTR mGL promoter reporter assay, which we used to test the effects of SOX binding site mutations +/- SOX protein overexpression. SOX6 increased L1 promoter activity, but only if the first of the two L1 5'UTR SOX motifs was intact.
January 30, 2024 at 10:58 AM
3. Development of a human L1 5'UTR mGL promoter reporter assay, which we used to test the effects of SOX binding site mutations +/- SOX protein overexpression. SOX6 increased L1 promoter activity, but only if the first of the two L1 5'UTR SOX motifs was intact.
2. Development of a mouse L1 5'RACE assay (inspired by the human L1 5'RACE developed by Deininger) to capture full-length mRNAs, corroborated by L1 ORF1p immunostaining. These assays showed full length, protein coding L1 TF mRNAs were abundant in PV neurons.
January 30, 2024 at 10:58 AM
2. Development of a mouse L1 5'RACE assay (inspired by the human L1 5'RACE developed by Deininger) to capture full-length mRNAs, corroborated by L1 ORF1p immunostaining. These assays showed full length, protein coding L1 TF mRNAs were abundant in PV neurons.
Some of the additional data:
1. Development of a Cre-LoxP conditional mouse L1spa (cL1spa) mCherry retrotransposition reporter and its use in primary mouse neurons, showing L1 mobility in the PV lineage, in agreement with our earlier in vivo data from a transgenic human L1.3 EGFP reporter.
1. Development of a Cre-LoxP conditional mouse L1spa (cL1spa) mCherry retrotransposition reporter and its use in primary mouse neurons, showing L1 mobility in the PV lineage, in agreement with our earlier in vivo data from a transgenic human L1.3 EGFP reporter.
January 30, 2024 at 10:57 AM
Some of the additional data:
1. Development of a Cre-LoxP conditional mouse L1spa (cL1spa) mCherry retrotransposition reporter and its use in primary mouse neurons, showing L1 mobility in the PV lineage, in agreement with our earlier in vivo data from a transgenic human L1.3 EGFP reporter.
1. Development of a Cre-LoxP conditional mouse L1spa (cL1spa) mCherry retrotransposition reporter and its use in primary mouse neurons, showing L1 mobility in the PV lineage, in agreement with our earlier in vivo data from a transgenic human L1.3 EGFP reporter.