Eric Strobel
@estrobel.bsky.social
Transcriptional acrobatics. Assistant Professor at the University at Buffalo.
Our paper describing a method for measuring cotranscriptional RNA folding using high-throughput RNA chemical probing is out @natcomms.nature.com!
June 5, 2025 at 2:32 PM
Our paper describing a method for measuring cotranscriptional RNA folding using high-throughput RNA chemical probing is out @natcomms.nature.com!
Apparently since the beginning!
December 28, 2023 at 2:39 AM
Apparently since the beginning!
We applied this to a ZTP riboswitch variant library to investigate the mechanisms by which variants that match the consensus sequence and structure misfold. Panel c illustrates the ZMP-mediated antitermination response for the 32,768 variants in the library!
December 26, 2023 at 4:00 PM
We applied this to a ZTP riboswitch variant library to investigate the mechanisms by which variants that match the consensus sequence and structure misfold. Panel c illustrates the ZMP-mediated antitermination response for the 32,768 variants in the library!
Our first TECdisplay assay measures transcription termination efficiency: TECs are tethered to a bead by the nascent RNA and DNA is released from the bead if transcription is terminated, but retained on the bead if RNA polymerase bypasses the terminator.
December 26, 2023 at 4:00 PM
Our first TECdisplay assay measures transcription termination efficiency: TECs are tethered to a bead by the nascent RNA and DNA is released from the bead if transcription is terminated, but retained on the bead if RNA polymerase bypasses the terminator.
About 6 years ago, I had an idea for measuring RNA function by using the activity of cotransciptionally displayed RNA to fractionate a transcription elongation complex (TEC) library. After fractionation, DNA would be recovered, barcoded, pooled back together, and sequenced
December 26, 2023 at 3:58 PM
About 6 years ago, I had an idea for measuring RNA function by using the activity of cotransciptionally displayed RNA to fractionate a transcription elongation complex (TEC) library. After fractionation, DNA would be recovered, barcoded, pooled back together, and sequenced
Paper data for an assay I dreamed up in 2018 👀
September 19, 2023 at 6:55 PM
Paper data for an assay I dreamed up in 2018 👀