We got our UASconstruct from David Lyons lab. The stable transgenic from Ryan Macdonald.

Such an elegant work, congrats to the entire team …

Those big and small spikes were certainly differently regulated in the neuromodulatory cells I patched . But again, I always thought I was listening to other

Super cool Anna… I remember patching some giant GnRH+ neurons in the olfactory bulb with different spike sizes . I always thought those smaller spikes were form a gap junction coupled neuron. But perhaps they were from a different AİS … btw what marker is used to label AİS here ?

thank you so much Fernando.. I truly appreciate the support.

Thanks …

Takes time to format, but worth it :)

Finally, big thanks to @kavlintnu.bsky.social , and The Research Council of Norway for funding support that made it possible to explore the evolution of cortical algorithms in our tiny fish.

Special thanks to Koichi Kawakami for sharing key transgenic lines, and to all our colleagues interested in cortical evolution, your insights and feedback were invaluable.

Our findings provide a comparative systems neuroscience perspective, showing how zebrafish can help us study thalamocortical computations in species with less accessible brains.

Inside the pallium, we found distinct computational layers, organized both topographically and hierarchically. Sensory processing complexity increases along the posterior-anterior axis, echoing organizational hierarchies seen in the mammalian cortex.

We show that the preglomerular nucleus (PG), architecturally similar to the higher-order thalamus in mammals and nucleus rotundus in birds/reptiles, is the main sensory input hub to the zebrafish pallium.

Thalamic inputs are key to functional regionalization in the mammalian cortex.
But in zebrafish?
We know surprisingly little about how their pallium receives and processes sensory input, aside from olfaction.

You might be thinking:
“Wait… cortex in fish??”
Well, zebrafish have a pallium, a brain region shared by all vertebrates that gave rise to the mammalian cortex over evolution.