Our study shows that duplex #sequencing can reliably detect #NGT genome-edited DNA down to 0.1%, suggesting a potential alternative detection approach when #PCR assay design is not feasible. 🧬🔎
tinyurl.com/duplexNGT
Our study shows that duplex #sequencing can reliably detect #NGT genome-edited DNA down to 0.1%, suggesting a potential alternative detection approach when #PCR assay design is not feasible. 🧬🔎
tinyurl.com/duplexNGT
At very low levels, however, precision dropped. We also observed some bias with certain mutations and samples, regardless of the level. ❌ 6/
At very low levels, however, precision dropped. We also observed some bias with certain mutations and samples, regardless of the level. ❌ 6/
Other unexpected mutations were found, but none was called in all replicates, and in both strands of at least two distinct DNA molecules. We could filter them out! ✅ 5/
Other unexpected mutations were found, but none was called in all replicates, and in both strands of at least two distinct DNA molecules. We could filter them out! ✅ 5/