David Breslow Lab
@davidbreslowlab.bsky.social
Cilia, centrosomes, signaling, and functional genomics - Yale Univ - Mol, Cell, Dev Biol. breslowlab.yale.edu
Great work by all authors, especially first Breslow lab grad student Shane Elliott. And thanks also to excellent collaborations at Yale with labs of Angelique Bordey and Tony Koleske
October 30, 2025 at 3:14 PM
Great work by all authors, especially first Breslow lab grad student Shane Elliott. And thanks also to excellent collaborations at Yale with labs of Angelique Bordey and Tony Koleske
3) it is noteworthy that a GOF CRISPRa screen was able to uncover a connection to a disorder caused by somatic activating mutations; possibly CRISPRa screening may be broadly useful for studying such diseases caused by somatic mutations ...
October 30, 2025 at 3:14 PM
3) it is noteworthy that a GOF CRISPRa screen was able to uncover a connection to a disorder caused by somatic activating mutations; possibly CRISPRa screening may be broadly useful for studying such diseases caused by somatic mutations ...
Potential implications: 1) aberrant cilia disassembly may be a recurrent feature of FCD that could contribute to pathogenesis, 2) excess cilia disassembly may be pathologic and could be considered a non-canonical basis for ciliopathy (typically caused by impaired formation of cilia), ...
October 30, 2025 at 3:14 PM
Potential implications: 1) aberrant cilia disassembly may be a recurrent feature of FCD that could contribute to pathogenesis, 2) excess cilia disassembly may be pathologic and could be considered a non-canonical basis for ciliopathy (typically caused by impaired formation of cilia), ...
As FCD is often caused by somatic GOF mutations, we wondered if aberrant cilia disassembly may occur in FCD. We found that FCD-linked mutations in SARM1 or RhoA act like GOF variants and potentiate cilia loss. Cilia loss via SARM1 also occurs for mTORC1-activating alterations commonly seen in FCD...
October 30, 2025 at 3:14 PM
As FCD is often caused by somatic GOF mutations, we wondered if aberrant cilia disassembly may occur in FCD. We found that FCD-linked mutations in SARM1 or RhoA act like GOF variants and potentiate cilia loss. Cilia loss via SARM1 also occurs for mTORC1-activating alterations commonly seen in FCD...
From hits, we defined a cilia disassembly pathway formed by GPCRs (F2R, LPAR1), SARM1 NADase, centrosomal Ca signaling, and RhoA.This pathway is necessary and sufficient for cilia disassembly.
Serendipitously, we noticed overlap between pathway components and genes mutated in an FCD cohort...
Serendipitously, we noticed overlap between pathway components and genes mutated in an FCD cohort...
October 30, 2025 at 3:14 PM
From hits, we defined a cilia disassembly pathway formed by GPCRs (F2R, LPAR1), SARM1 NADase, centrosomal Ca signaling, and RhoA.This pathway is necessary and sufficient for cilia disassembly.
Serendipitously, we noticed overlap between pathway components and genes mutated in an FCD cohort...
Serendipitously, we noticed overlap between pathway components and genes mutated in an FCD cohort...
It seems there are many type 4 R01s issuing mult years of funding at once, possibly in lieu of standard non-competing renewals (i.e. MYF). I see ~220 awards, >450 grant-years, >$175m direct costs. I'm not familiar with type 4, but looks like ~10X as many since May than all of last year.
September 9, 2025 at 10:18 PM
It seems there are many type 4 R01s issuing mult years of funding at once, possibly in lieu of standard non-competing renewals (i.e. MYF). I see ~220 awards, >450 grant-years, >$175m direct costs. I'm not familiar with type 4, but looks like ~10X as many since May than all of last year.
That is very helpful! Colleague here was just persuaded to pay a >$10k APC for a Cell Press journal - nice to know this may be unnecessary
July 28, 2025 at 8:05 PM
That is very helpful! Colleague here was just persuaded to pay a >$10k APC for a Cell Press journal - nice to know this may be unnecessary
Nice that JCB / RUP has clear guidance with their ‘Green’ option. Any idea if Cell Press or Nature journals allow this?
July 28, 2025 at 7:25 PM
Nice that JCB / RUP has clear guidance with their ‘Green’ option. Any idea if Cell Press or Nature journals allow this?
Application at NINDS now shows Apr 10 council date in eRA Commons. NIGMS application shows May 22, which is the date that was planned by GM for the following cycle. Both apps were originally supposed to have Council in Feb...
March 20, 2025 at 2:36 AM
Application at NINDS now shows Apr 10 council date in eRA Commons. NIGMS application shows May 22, which is the date that was planned by GM for the following cycle. Both apps were originally supposed to have Council in Feb...