David Breslow Lab
@davidbreslowlab.bsky.social
Cilia, centrosomes, signaling, and functional genomics - Yale Univ - Mol, Cell, Dev Biol. breslowlab.yale.edu
Reposted by David Breslow Lab
We built a targeted protein degradation–based system to mimic reproductive age-related aneuploidy in young eggs - revealing how chromosome errors associated with female infertility arise with age. 🧬✨
With @jiyeonleem.bsky.social and our team at Yale MCDB.
Read: www.nature.com/articles/s43...
With @jiyeonleem.bsky.social and our team at Yale MCDB.
Read: www.nature.com/articles/s43...
A versatile cohesion manipulation system probes female reproductive age-related egg aneuploidy - Nature Aging
To study pathways that lead to aneuploidy during aging, the authors provide a system that enables cohesion protein depletion in mouse oocytes, mimicking effects that occur during aging. They uncover a...
www.nature.com
November 4, 2025 at 1:17 PM
We built a targeted protein degradation–based system to mimic reproductive age-related aneuploidy in young eggs - revealing how chromosome errors associated with female infertility arise with age. 🧬✨
With @jiyeonleem.bsky.social and our team at Yale MCDB.
Read: www.nature.com/articles/s43...
With @jiyeonleem.bsky.social and our team at Yale MCDB.
Read: www.nature.com/articles/s43...
Excited to share our latest work on a new cilia disassembly pathway and a link between this pathway and the neurological disorder focal cortical dysplasia: www.science.org/doi/10.1126/...
Brief summary: we used a genome-wide CRISPRa GOF screen to identify negative regulators of ciliary signaling...
Brief summary: we used a genome-wide CRISPRa GOF screen to identify negative regulators of ciliary signaling...
A CRISPR activation screen reveals a cilia disassembly pathway mutated in focal cortical dysplasia
A gain-of-function screen uncovers a cilia disassembly pathway linked to genes somatically mutated in neurodevelopmental disease.
www.science.org
October 30, 2025 at 3:14 PM
Excited to share our latest work on a new cilia disassembly pathway and a link between this pathway and the neurological disorder focal cortical dysplasia: www.science.org/doi/10.1126/...
Brief summary: we used a genome-wide CRISPRa GOF screen to identify negative regulators of ciliary signaling...
Brief summary: we used a genome-wide CRISPRa GOF screen to identify negative regulators of ciliary signaling...
Reposted by David Breslow Lab
I'm thrilled to share our new preprint, in which we've found that neurotransmitters can be chemically "marked" as a way to incorporate metabolic history - such as diet or life stage. www.biorxiv.org/content/10.1... Led by Porhathai "Un" Malaiwong, Allen Schroeder and Tia Brown. (1/6) 🧵
Nuclear receptor-neurotransmitter coupling links behavior to metabolic state
Animals must flexibly respond to environmental stimuli to survive, and optimal responses critically depend on the organism's current needs. Many organisms have evolved both cell-intrinsic and intertis...
www.biorxiv.org
October 24, 2025 at 4:28 PM
I'm thrilled to share our new preprint, in which we've found that neurotransmitters can be chemically "marked" as a way to incorporate metabolic history - such as diet or life stage. www.biorxiv.org/content/10.1... Led by Porhathai "Un" Malaiwong, Allen Schroeder and Tia Brown. (1/6) 🧵
Reposted by David Breslow Lab
Calling all biologists! 📢
Is your research at the cutting edge of synthetic biology, genomics or AI?
Apply to join our team of world-class scholars!🔬🦠
Tenure-track Assistant Professor
Anticipated Start Date: July 1, 2026
#hiring #genomics #biology
apply.interfolio.com/172428
Is your research at the cutting edge of synthetic biology, genomics or AI?
Apply to join our team of world-class scholars!🔬🦠
Tenure-track Assistant Professor
Anticipated Start Date: July 1, 2026
#hiring #genomics #biology
apply.interfolio.com/172428
Apply - Interfolio
{{$ctrl.$state.data.pageTitle}} - Apply - Interfolio
apply.interfolio.com
September 9, 2025 at 6:50 PM
Calling all biologists! 📢
Is your research at the cutting edge of synthetic biology, genomics or AI?
Apply to join our team of world-class scholars!🔬🦠
Tenure-track Assistant Professor
Anticipated Start Date: July 1, 2026
#hiring #genomics #biology
apply.interfolio.com/172428
Is your research at the cutting edge of synthetic biology, genomics or AI?
Apply to join our team of world-class scholars!🔬🦠
Tenure-track Assistant Professor
Anticipated Start Date: July 1, 2026
#hiring #genomics #biology
apply.interfolio.com/172428
Reposted by David Breslow Lab
Very few PhD students finish within 4 years. But international students will need to do that if US proposed visa rules change go through.
Sound reasonable to you?
Fed register is accepting comments for next month. Details at the link & comment button
www.federalregister.gov/documents/20...
Sound reasonable to you?
Fed register is accepting comments for next month. Details at the link & comment button
www.federalregister.gov/documents/20...
Establishing a Fixed Time Period of Admission and an Extension of Stay Procedure for Nonimmigrant Academic Students, Exchange Visitors, and Representatives of Foreign Information Media
Unlike most nonimmigrant classifications, which are admitted for a fixed time period, aliens in the F (academic student), J (exchange visitor), and most I (representatives of foreign information media...
www.federalregister.gov
August 29, 2025 at 6:32 AM
Very few PhD students finish within 4 years. But international students will need to do that if US proposed visa rules change go through.
Sound reasonable to you?
Fed register is accepting comments for next month. Details at the link & comment button
www.federalregister.gov/documents/20...
Sound reasonable to you?
Fed register is accepting comments for next month. Details at the link & comment button
www.federalregister.gov/documents/20...
Reposted by David Breslow Lab
“The Senate Committee on Appropriations is scheduled to release its proposal for NIH’s 2026 budget later this week…The committee could include language that would prohibit the multiyear funding plan or extend the timeline for the transition.”🧪
New Science story about multi-year funding and it potential catastrophic consequences
www.science.org/content/arti...
1/n
www.science.org/content/arti...
1/n
Odds of winning NIH grants plummet as new funding policy and spending delays bite
Funding multiyear grants up front will sharply cut number of investigators receiving awards
www.science.org
July 29, 2025 at 1:02 PM
“The Senate Committee on Appropriations is scheduled to release its proposal for NIH’s 2026 budget later this week…The committee could include language that would prohibit the multiyear funding plan or extend the timeline for the transition.”🧪
Reposted by David Breslow Lab
Want to publish a NIH-funded paper in Nature? Well - unless your institution has an agreement with Springer, you'll have to be mega-rich to be compliant with NIH's new no-embargo policy. Springer is insisting on gold OA - that's $12,690 and that's extortion.
This is a useful summary of how some major publishers are responding to the new NIH policy. We've just updated our policies at @biologists.bsky.social: @dev-journal.bsky.social @jcellsci.bsky.social and @jexpbiol.bsky.social will allow authors to deposit the accepted version with zero embargo.
July 29, 2025 at 1:12 PM
Want to publish a NIH-funded paper in Nature? Well - unless your institution has an agreement with Springer, you'll have to be mega-rich to be compliant with NIH's new no-embargo policy. Springer is insisting on gold OA - that's $12,690 and that's extortion.
Reposted by David Breslow Lab
ACTION ITEM-----ACTION ITEM
Implementation of Schedule F
This is what a lot of us have been worried about.
This allows for many civil service positions to replaced with political appointees. This could include NIH institute directors and even POs.
BUT THERE IS A COMMENT PERIOD...
1/n
Implementation of Schedule F
This is what a lot of us have been worried about.
This allows for many civil service positions to replaced with political appointees. This could include NIH institute directors and even POs.
BUT THERE IS A COMMENT PERIOD...
1/n
the word alert is in a purple circle
ALT: the word alert is in a purple circle
media.tenor.com
April 23, 2025 at 6:03 PM
ACTION ITEM-----ACTION ITEM
Implementation of Schedule F
This is what a lot of us have been worried about.
This allows for many civil service positions to replaced with political appointees. This could include NIH institute directors and even POs.
BUT THERE IS A COMMENT PERIOD...
1/n
Implementation of Schedule F
This is what a lot of us have been worried about.
This allows for many civil service positions to replaced with political appointees. This could include NIH institute directors and even POs.
BUT THERE IS A COMMENT PERIOD...
1/n
Reposted by David Breslow Lab
We're live! @inikon.bsky.social used modular proximity labeling to identify ciliary EV cargo in #celegans. This EV-TurboID approach can be applied in cell- & tissue-specific manners to define the composition of distinct EV subtypes, a major challenge of the EV field.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
April 3, 2025 at 1:03 PM
We're live! @inikon.bsky.social used modular proximity labeling to identify ciliary EV cargo in #celegans. This EV-TurboID approach can be applied in cell- & tissue-specific manners to define the composition of distinct EV subtypes, a major challenge of the EV field.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by David Breslow Lab
Reposted by David Breslow Lab
Trypanosome doublet microtubule structures reveal flagellum assembly and motility mechanisms | Science www.science.org/doi/10.1126/...
Trypanosome doublet microtubule structures reveal flagellum assembly and motility mechanisms
The flagellum of Trypanosoma brucei drives the parasite’s characteristic screw-like motion and is essential for its replication, transmission, and pathogenesis. However, the molecular details of this ...
www.science.org
March 13, 2025 at 7:57 PM
Trypanosome doublet microtubule structures reveal flagellum assembly and motility mechanisms | Science www.science.org/doi/10.1126/...
Reposted by David Breslow Lab
Congrats to Anna Seminara on her paper out today in @cp-cellchembiol.bsky.social reporting a new approach to metabolite depletion using a bacterial ABC transporter, applied to study the antioxidant ergothioneine in H. pylori!
Online now! An affinity-based depletion strategy for evaluating the effects of ergothioneine on bacterial physiology by Anna B. Seminara and Stavroula K. Hatzios @hatzioslab.bsky.social at @yale.edu @mcdb-yale.bsky.social http://dlvr.it/TJRWKG
March 10, 2025 at 7:23 PM
Congrats to Anna Seminara on her paper out today in @cp-cellchembiol.bsky.social reporting a new approach to metabolite depletion using a bacterial ABC transporter, applied to study the antioxidant ergothioneine in H. pylori!
Reposted by David Breslow Lab
CT folks! Let’s come together and make signs for #standupforscience! @luskinglab.bsky.social
March 1, 2025 at 10:36 PM
CT folks! Let’s come together and make signs for #standupforscience! @luskinglab.bsky.social
Reposted by David Breslow Lab
Some good news during these difficult times. Please check out our new preprint. We built a new tool to probe the origins of high oocyte aneuploidy rates at advanced female reproductive ages. This NIH funded work was led by a fantastic postdoc in the lab, Jiyeon Leem. www.biorxiv.org/content/10.1...
A versatile cohesion manipulation system reveals CENP-A dysfunction accelerates female reproductive age-related egg aneuploidy.
Female reproductive aging is accompanied by a dramatic rise in the incidence of egg aneuploidy. Premature loss of chromosome cohesion proteins and untimely separation of chromosomes is thought to unde...
www.biorxiv.org
February 28, 2025 at 12:39 PM
Some good news during these difficult times. Please check out our new preprint. We built a new tool to probe the origins of high oocyte aneuploidy rates at advanced female reproductive ages. This NIH funded work was led by a fantastic postdoc in the lab, Jiyeon Leem. www.biorxiv.org/content/10.1...
Reposted by David Breslow Lab
Trump's illegal NIH cuts mean cancer researchers would be laid off, sick kids going without treatment, and more. These cuts would be DEVASTATING for lifesaving research.
Some Republicans have said they agree—but they just blocked my amendment to reverse Trump's NIH cuts anyway.
Some Republicans have said they agree—but they just blocked my amendment to reverse Trump's NIH cuts anyway.
February 21, 2025 at 9:27 AM
Trump's illegal NIH cuts mean cancer researchers would be laid off, sick kids going without treatment, and more. These cuts would be DEVASTATING for lifesaving research.
Some Republicans have said they agree—but they just blocked my amendment to reverse Trump's NIH cuts anyway.
Some Republicans have said they agree—but they just blocked my amendment to reverse Trump's NIH cuts anyway.
Reposted by David Breslow Lab
Trump Administration Stalls Scientific Research Despite Court Ruling. Story by Ben Mueller. Gift link: nyti.ms/435szDm
Trump Administration Stalls Scientific Research Despite Court Ruling (Gift Article)
Using an arcane law, officials have effectively delayed funding from the National Institutes of Health, leaving medical studies in jeopardy.
nyti.ms
February 21, 2025 at 10:42 PM
Trump Administration Stalls Scientific Research Despite Court Ruling. Story by Ben Mueller. Gift link: nyti.ms/435szDm
Reposted by David Breslow Lab
Almost all grant-review meetings under Trump 2.0 remain suspended at the US National Institutes of Health (NIH), preventing the world’s largest public funder of biomedical research from spending much of its US$47 billion annual budget.
https://go.nature.com/4gM6oW4
https://go.nature.com/4gM6oW4
Revealed: NIH research grants still frozen despite lawsuits challenging Trump order
The Trump administration is exploiting a loophole to keep a funding freeze in place, leaving researchers in limbo.
go.nature.com
February 20, 2025 at 8:04 PM
Almost all grant-review meetings under Trump 2.0 remain suspended at the US National Institutes of Health (NIH), preventing the world’s largest public funder of biomedical research from spending much of its US$47 billion annual budget.
https://go.nature.com/4gM6oW4
https://go.nature.com/4gM6oW4
Reposted by David Breslow Lab
So can anyone shed light on how long they can legally keep the NIH communications freeze in place? It's been a long time since February 1 came and went. Is there any legal recourse?
February 20, 2025 at 2:22 AM
So can anyone shed light on how long they can legally keep the NIH communications freeze in place? It's been a long time since February 1 came and went. Is there any legal recourse?
Reposted by David Breslow Lab
A large portion of grants awarded by the U.S. National Institutes of Health cannot be funded for the foreseeable future because of an indefinite hold on submissions to the Federal Register, according to an email reviewed by The Transmitter.
By @avaskham.bsky.social
bit.ly/3X8ngz8
By @avaskham.bsky.social
bit.ly/3X8ngz8
Federal Register hold makes ‘end run’ around court pause on NIH funding freeze
NIH-related updates to the Federal Register, which are required for the scheduling of study sections and advisory councils, are on hold indefinitely, according to an email reviewed by <i>The Transmitt...
www.thetransmitter.org
February 18, 2025 at 11:23 PM
A large portion of grants awarded by the U.S. National Institutes of Health cannot be funded for the foreseeable future because of an indefinite hold on submissions to the Federal Register, according to an email reviewed by The Transmitter.
By @avaskham.bsky.social
bit.ly/3X8ngz8
By @avaskham.bsky.social
bit.ly/3X8ngz8
Reposted by David Breslow Lab
Tomorrow!! Join us in New Haven, CT at 5:15 on the green or find one near you. @aaup.bsky.social
February 18, 2025 at 10:56 PM
Tomorrow!! Join us in New Haven, CT at 5:15 on the green or find one near you. @aaup.bsky.social
Reposted by David Breslow Lab
I still don't think it's sunk in to many that after February 20th, there ain't no study sections.
February 16, 2025 at 7:05 PM
I still don't think it's sunk in to many that after February 20th, there ain't no study sections.
Looks like today's NINDS council meeting was canceled...
February 12, 2025 at 5:56 PM
Looks like today's NINDS council meeting was canceled...
Reposted by David Breslow Lab
NEW: The N.I.H. will cut about $4 Billion from federal research grants that support cancer, virus and heart disease research.
#Project2025 called for the cuts to end subsidies to "leftist" university agendas.
www.nytimes.com/2025/02/07/u...
#Project2025 called for the cuts to end subsidies to "leftist" university agendas.
www.nytimes.com/2025/02/07/u...
Deep Cuts to Medical Research Funds Could Hobble University Budgets
Grants from the National Institutes of Health come with additional money for overhead. Proposed funding cuts would leave colleges with large budget gaps.
www.nytimes.com
February 8, 2025 at 2:17 AM
NEW: The N.I.H. will cut about $4 Billion from federal research grants that support cancer, virus and heart disease research.
#Project2025 called for the cuts to end subsidies to "leftist" university agendas.
www.nytimes.com/2025/02/07/u...
#Project2025 called for the cuts to end subsidies to "leftist" university agendas.
www.nytimes.com/2025/02/07/u...
Reposted by David Breslow Lab
This is catastrophic for US research universities as it ignores the real cost of doing research. The US academic-gov’t partnership forged post-WWII is the greatest creator of knowledge in history and is critical to US economic and technological competitiveness.
February 8, 2025 at 1:31 AM
This is catastrophic for US research universities as it ignores the real cost of doing research. The US academic-gov’t partnership forged post-WWII is the greatest creator of knowledge in history and is critical to US economic and technological competitiveness.
Reposted by David Breslow Lab
Proud to share our work spear-headed by PhD student Tommy Sroka with major contributions by #xenopus expert Kerstin Feistel.
Our iAPEX #ProximityLabeling method for #MassSpec based subcellular #proteomics works by locally generating H2O2 using a D-amino acid oxidase that activates APEX2 in situ.
Our iAPEX #ProximityLabeling method for #MassSpec based subcellular #proteomics works by locally generating H2O2 using a D-amino acid oxidase that activates APEX2 in situ.
iAPEX: Improved APEX-based proximity labeling for subcellular proteomics using an enzymatic reaction cascade
Ascorbate peroxidase (APEX) is a versatile labeling enzyme used for live-cell proteomics at high spatial and temporal resolution. However, toxicity of its substrate hydrogen peroxide and background la...
www.biorxiv.org
January 11, 2025 at 7:51 PM
Proud to share our work spear-headed by PhD student Tommy Sroka with major contributions by #xenopus expert Kerstin Feistel.
Our iAPEX #ProximityLabeling method for #MassSpec based subcellular #proteomics works by locally generating H2O2 using a D-amino acid oxidase that activates APEX2 in situ.
Our iAPEX #ProximityLabeling method for #MassSpec based subcellular #proteomics works by locally generating H2O2 using a D-amino acid oxidase that activates APEX2 in situ.