Thanks Mathieu!

Thanks to the reviewers and the editor @katciazynska.bsky.social

This was led by Alzbeta Roeselova and Santosh Shivakumaraswamy in my lab. Cryo-EM by Gabija Jurkeviciute and Jessica He in Rado Enchev's lab, and selRiboseq from Bernd Bukau's lab.

Functional assembly starts during translation, and in a surprising way. Although our model protein is a homooligomer, assembly is directional.
One fully-synthesised subunit interacts with an incomplete subunit on the ribosome, avoiding misassembly between two incomplete chains.

A hidden hydrophobic groove on the ribosome surface, normally capped by a flexible "lid", can stabilise nascent amphipathic helices and change the pathway of domain folding.

A hidden hydrophobic groove on the ribosome surface, normally capped by a flexible "lid", can stabilise nascent amphipathic helices and change the pathway of domain folding.