For previous research on #FutureAntibiotics and resistance development see our recent publications here: tinyurl.com/2cbyrx55 and tinyurl.com/2mxaz5a6 #MEvoSky

5/5 - Contributors:
This work was spearheaded by @m-elvin.bsky.social and @marczikkely.bsky.social at HUN-REN Biological Research Centre, Szeged

4/5 - Implications for the Future:
These insights provide a blueprint for developing next-gen antibiotics ( #FutureAntibiotics) designed to stay one step ahead of bacterial defenses. It’s a promising direction in the fight against #AntibioticResistance #AMR.

3/5 - A stark contrast:
Dual-target topoisomerase antibiotics, despite having two intracellular targets, were more prone to resistance. This highlights that which targets matter just as much as how many. #DrugDevelopment

2/5 – Dual target permeabilizers:
We tested three promising #DrugCandidates – POL7306, Tridecaptin M152-P3, and SCH79797 – against tough #ESKAPE pathogens like E. coli, K. pneumoniae, A. baumannii, and P. aeruginosa. The results? #ResistanceEvolution was significantly limited.

1/5 - The strategy:
By combining membrane disruption with another key cellular pathway, we can dramatically limit bacterial #resistance. This “double whammy” makes it much harder for bacteria to fight back. #RationalDesign #Antibiotics

For a similar study on Gram-positive bacteria, check out our previous thread: bsky.app/profile/csab...

7/7 🔗 This study spearheaded by @lejladaruka.bsky.social, @marczikkely.bsky.social, @petraszili.bsky.social and @zoltanfarkas81.bsky.social at Biological Research Centre, Szeged. Important contributions from our long-term collaborators @balazs-papp-lab.bsky.social and Balint Kintses Lab.

6/7 📢 Stay tuned! Our upcoming study will reveal a promising strategy to develop less resistance-prone antibiotics! #DrugDevelopment

5/7 🔬 The study suggests that certain bacteria were less prone to develop resistance against some of the tested antibiotics, highlighting the potential in narrow-spectrum therapeutics. This presents hope for more effective future treatments. #Antibiotics #PublicHealth

4/7 🌍 Functional metagenomics revealed that mobile resistance genes exist not only in clinical isolates but also in the soil and even the human gut microbiome. Resistance is EVERYWHERE, making it harder to tackle with new treatments. #GlobalHealth

3/7 🧬 Even more worrying: resistance mutations were already present in natural bacterial populations. This means that some bacteria may already have the arsenal to resist future antibiotics they have never encountered. #EvolutionOfResistance

2/7 🦠 We tested 13 new antibiotics (some on the market for a couple of years, others still in development) against 4 of the most critical Gram-negative pathogens. The result? Resistance emerged in just 60 days. This shows how quickly bacteria can adapt! #AntibioticResistance

1/7 The rise of drug-resistant infections is a global crisis, but many pharma companies have abandoned antibiotic R&D. While some new drugs are in development, our study shows resistance is emerging rapidly, even to these #FutureAntibiotics. Let’s unpack the results.⬇️

This work was spearheaded by Ana Martins, Fanni Judak and @zoltanfarkas81.bsky.social at Biological Research Centre, Szeged. Stay tuned for upcoming updates on #FutureAntibiotics - some exciting stuff on the horizon.