@coldpopeye.bsky.social
Things big and small. And smaller than that.
Reposted
Paper alert🚨
Label your protein of interest and localize it in cryoET 👁️❄️
Genetically encoded FerriTag as a specific label for cryo-electron tomography: Structure www.cell.com/structure/fu...
Label your protein of interest and localize it in cryoET 👁️❄️
Genetically encoded FerriTag as a specific label for cryo-electron tomography: Structure www.cell.com/structure/fu...
Genetically encoded FerriTag as a specific label for cryo-electron tomography
Wang et al. present an optimized labeling strategy for cryo-electron tomography based
on genetically encoded FerriTag. This system uses rapamycin to recruit iron-loaded
ferritin to target proteins, en...
www.cell.com
September 11, 2025 at 9:36 AM
Paper alert🚨
Label your protein of interest and localize it in cryoET 👁️❄️
Genetically encoded FerriTag as a specific label for cryo-electron tomography: Structure www.cell.com/structure/fu...
Label your protein of interest and localize it in cryoET 👁️❄️
Genetically encoded FerriTag as a specific label for cryo-electron tomography: Structure www.cell.com/structure/fu...
Reposted
In collaboration with the Posthaus Lab, we identify ANTXR2—also the anthrax toxin receptor—as the receptor for Clostridium perfringens NetF pore forming toxin. Cryo-EM shows extensive contact between the pore and the receptor
🧬🧊🔬 #Microbiology #CryoEM #PoreFormingToxins
doi.org/10.1101/2025...
🧬🧊🔬 #Microbiology #CryoEM #PoreFormingToxins
doi.org/10.1101/2025...
July 4, 2025 at 7:50 AM
In collaboration with the Posthaus Lab, we identify ANTXR2—also the anthrax toxin receptor—as the receptor for Clostridium perfringens NetF pore forming toxin. Cryo-EM shows extensive contact between the pore and the receptor
🧬🧊🔬 #Microbiology #CryoEM #PoreFormingToxins
doi.org/10.1101/2025...
🧬🧊🔬 #Microbiology #CryoEM #PoreFormingToxins
doi.org/10.1101/2025...
Reposted
Anthrax toxin receptor 2 is the Receptor for Clostridium perfringens NetF: Structural Insights into Toxin Binding and Pore Formation https://www.biorxiv.org/content/10.1101/2025.07.02.662835v1
July 3, 2025 at 3:16 AM
Anthrax toxin receptor 2 is the Receptor for Clostridium perfringens NetF: Structural Insights into Toxin Binding and Pore Formation https://www.biorxiv.org/content/10.1101/2025.07.02.662835v1
Reposted
Out now at IUCJ doi.org/10.1107/S205...
June 25, 2025 at 7:36 AM
Out now at IUCJ doi.org/10.1107/S205...
Reposted
Stronger together through Erasmus+.
#ErasmusPlus has received the European Integration Award from the Cercle d’Economia.
🏆 The award honours 35+ years of empowering people, breaking down barriers and uniting Europe through learning and shared values.
To all the changemakers involved, thank you!
#ErasmusPlus has received the European Integration Award from the Cercle d’Economia.
🏆 The award honours 35+ years of empowering people, breaking down barriers and uniting Europe through learning and shared values.
To all the changemakers involved, thank you!
May 12, 2025 at 3:00 PM
Stronger together through Erasmus+.
#ErasmusPlus has received the European Integration Award from the Cercle d’Economia.
🏆 The award honours 35+ years of empowering people, breaking down barriers and uniting Europe through learning and shared values.
To all the changemakers involved, thank you!
#ErasmusPlus has received the European Integration Award from the Cercle d’Economia.
🏆 The award honours 35+ years of empowering people, breaking down barriers and uniting Europe through learning and shared values.
To all the changemakers involved, thank you!
Reposted
1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.
April 27, 2025 at 8:49 PM
1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.
Reposted
Great stuff by @jgreener64.bsky.social and @kjamali.bsky.social: search protein structures in 0.1 seconds using their open software!
This has been a long journey, but it's now out in final form. @kjamali.bsky.social
academic.oup.com/bioinformati...
academic.oup.com/bioinformati...
April 8, 2025 at 9:47 PM
Great stuff by @jgreener64.bsky.social and @kjamali.bsky.social: search protein structures in 0.1 seconds using their open software!
Reposted
Reposted
Happy to announce our latest preprint. High resolution ribosomes in cells obtained on CEMOVIS (frozen-hydrated sections) www.biorxiv.org/content/10.1... in collaboration with @grigoriefflab.bsky.social and Wanda Kukulski.
April 1, 2025 at 6:18 AM
Happy to announce our latest preprint. High resolution ribosomes in cells obtained on CEMOVIS (frozen-hydrated sections) www.biorxiv.org/content/10.1... in collaboration with @grigoriefflab.bsky.social and Wanda Kukulski.
Reposted
March 31, 2025 at 8:27 PM
Reposted
High-resolution cryo-EM structures of a protein pore reveal diverse roles of membrane lipids https://www.biorxiv.org/content/10.1101/2024.06.26.600493v1
High-resolution cryo-EM structures of a protein pore reveal diverse roles of membrane lipids https://www.biorxiv.org/content/10.1101/2024.06.26.600493v1
The structure and function of membrane proteins depend on their interactions with the lipid molecule
www.biorxiv.org
June 26, 2024 at 1:46 PM
High-resolution cryo-EM structures of a protein pore reveal diverse roles of membrane lipids https://www.biorxiv.org/content/10.1101/2024.06.26.600493v1
Reposted
Pore lipid modifications modulate MscS nanopore for enhanced single-molecule sensing pubmed.ncbi.nlm.nih.gov/39955838/ #cryoem
February 18, 2025 at 4:18 AM
Pore lipid modifications modulate MscS nanopore for enhanced single-molecule sensing pubmed.ncbi.nlm.nih.gov/39955838/ #cryoem
Reposted
Excited to share our new preprint "Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons" www.biorxiv.org/content/10.1...
We combine optogenetic stimulation of neurons with in situ cryo-ET to characterize membrane dynamics during synaptic vesicle fusion ❄️🔬⏱️
We combine optogenetic stimulation of neurons with in situ cryo-ET to characterize membrane dynamics during synaptic vesicle fusion ❄️🔬⏱️
February 12, 2025 at 10:05 PM
Excited to share our new preprint "Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons" www.biorxiv.org/content/10.1...
We combine optogenetic stimulation of neurons with in situ cryo-ET to characterize membrane dynamics during synaptic vesicle fusion ❄️🔬⏱️
We combine optogenetic stimulation of neurons with in situ cryo-ET to characterize membrane dynamics during synaptic vesicle fusion ❄️🔬⏱️
Reposted
Our latest JACS paper: high-resolution cryoEM of #aerolysin in lipid environment shows pore properties and 4 key constriction rings for #nanopore sensing. Collaboration with #DalPeraro & #Cao labs.
@coldpopeye.bsky.social
@unibern.bsky.social
#EPFL #UNIGE #DubochetCenter #MIC
shorturl.at/qyzbb
@coldpopeye.bsky.social
@unibern.bsky.social
#EPFL #UNIGE #DubochetCenter #MIC
shorturl.at/qyzbb
Aerolysin Nanopore Structures Revealed at High Resolution in a Lipid Environment
Aerolysin is a β-pore-forming toxin produced by most Aeromonas bacteria, which has attracted large attention in the field of nanopore sensing due to its narrow and charged pore lumen. Structurally sim...
shorturl.at
February 4, 2025 at 8:28 AM
Our latest JACS paper: high-resolution cryoEM of #aerolysin in lipid environment shows pore properties and 4 key constriction rings for #nanopore sensing. Collaboration with #DalPeraro & #Cao labs.
@coldpopeye.bsky.social
@unibern.bsky.social
#EPFL #UNIGE #DubochetCenter #MIC
shorturl.at/qyzbb
@coldpopeye.bsky.social
@unibern.bsky.social
#EPFL #UNIGE #DubochetCenter #MIC
shorturl.at/qyzbb
Reposted
1/Interested in biomolecular condensates? If yes, check 10.1073/pnas.2419610121 where we describe the in situ architecture of the PomXYZ co-condensate using cryo-correlative light and electron microscopy & in situ cryo-ET
Wonderful coll/w Baumeister lab @madic.bsky.social
#teamtomo #cryoET #cryoEM
Wonderful coll/w Baumeister lab @madic.bsky.social
#teamtomo #cryoET #cryoEM
January 3, 2025 at 10:32 AM
1/Interested in biomolecular condensates? If yes, check 10.1073/pnas.2419610121 where we describe the in situ architecture of the PomXYZ co-condensate using cryo-correlative light and electron microscopy & in situ cryo-ET
Wonderful coll/w Baumeister lab @madic.bsky.social
#teamtomo #cryoET #cryoEM
Wonderful coll/w Baumeister lab @madic.bsky.social
#teamtomo #cryoET #cryoEM