Congratulations, Emilie — such exciting work!

Grateful to all our collaborators, @elena-dvoretskova.bsky.social, Diana Rodrigues, Michael Myoga, Chao Feng, Ilaraia Vitali, Elif Dönmez and Christian Peters for their contributions to this work!

Explore the data in our interactive app: mayerlab.net/mouse-inhibi...

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Gene regulatory network analysis pointed to NFIB as a central hub. It interacts e.g. with MEIS2 and TCF4, and drives late-stage enhancer activation. Heterochronic transplants suggests: maturation competence is sensitive to environmental timing.
#GeneRegulation #Scenic+ #NFIB #Transplantation
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We also saw a shift in TF activity.

Footprinting showed increased NFI binding, especially NFIB, in late-born cohorts. These neurons activate maturation modules faster — possibly guided by chromatin remodeling during neurogenesis.
#TFfootprints #TranscriptionFactors #scATACseq
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This shift was mirrored in chromatin accessibility.

scATAC-seq revealed stage-specific enhancer activation, with late-born cells opening distinct regulatory elements — especially in distal regions. A dynamic change in the regulatory landscape.
#Chromatin #Epigenetics #scATACseq
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Using FlashTag to label isochronic cohorts, we tracked the fate of early- and late-born GABAergic neurons. Surprisingly, later-born neurons matured faster — reaching advanced transcriptional states in hours rather than days. Differentiation stayed relatively constant; maturation didn’t.
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During corticogenesis, progenitor competence changes over time — generating distinct excitatory neuron types.
But it turns out that in inhibitory lineages, progenitors retain more stable differentiation competence. Instead, temporal progression alters maturation dynamics.
#PatchClamp
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This project is co-funded by the Nancy Lurie Marks Family Foundation (NLMFF). Grateful for their support in advancing research on neurodevelopment and autism spectrum disorders.

More about the grant: www.nlmfoundation.org/grant/epigen...

#NLMFF #AutismResearch #Neurodevelopment