Many thanks to all the people that provided support to this work: @jacquet-chris.bsky.social, @baptistebio.bsky.social, C. Dunand S. Cheng, H. San Clemente, H. Philippe, S. Aziz ! (18/18)

This gene family, probably involved in the drought response of M. polymorpha, originates from a horizontal gene transfer from a fungi to the ancestor of land plant, later lost in seed plants. Studying Marchantia therefore enabled us to further understand how plants conquered the land! (14/18)

Focusing on the M. polymorpha ssp. ruderalis pangenome, we took a look at the accessory genes, displaying presence absence variation between the accessions. A functional enrichment reveals that it is linked to response to stress, similarly to what has been observed in angiosperms (12/18)

Finally, we constructed a pangenome to visualise the presence absence of genes in the different accessions. We found genetic features shared by all accessions in the 3 Marchantia subspecies and the sister species, Marchantia paleacea, a glimpse into the “basic Marchantia toolkit” (11/18)

Performing a Genome x Environment Analysis, we identified loci correlated with climatic variables in Marchantia polymorpha, among which a loci encoding for an ABC1 atypical kinase, that could be involved in abscisic acid-related stress response. And peroxidases (again). And a NLR. (10/18)

Then we identified genes under selective pressure in M. polymorpha, and noticed that Marchantia’s polymorphic genes are likely to mediate the interactions between the plant and its environment (e.g. pathogenesis related proteins, lectins or peroxidases) (7/18)

First, we took a look at the population structure of M. polymorpha. There is no genetic x geographic correlation, suggesting gene flow and recombination across the large geographical range of M. polymorpha ssp. ruderalis (6/18)

But also from Northern America (J. Nelson) and from Japan, distributed between the three subspecies of Marchantia polymorpha (16 from ssp. montivagans, 14 from ssp. polymorpha and 103 from ssp. ruderalis) (3/18)