Chris Ashwood
@cashwood.proteaglyco.com
Director of Protea Glycosciences
Accelerating glycoscience research
Accelerating glycoscience research
To use it, it's best to search for the instrument model and work from there.
Here's an example of all Orbitrap Astral (Zoom) tenders with purchase values:
The $1 is likely a placeholder, but MPI may not be.
These can also include LC, service contracts, etc, so approximation at best
Here's an example of all Orbitrap Astral (Zoom) tenders with purchase values:
The $1 is likely a placeholder, but MPI may not be.
These can also include LC, service contracts, etc, so approximation at best
November 10, 2025 at 12:21 PM
To use it, it's best to search for the instrument model and work from there.
Here's an example of all Orbitrap Astral (Zoom) tenders with purchase values:
The $1 is likely a placeholder, but MPI may not be.
These can also include LC, service contracts, etc, so approximation at best
Here's an example of all Orbitrap Astral (Zoom) tenders with purchase values:
The $1 is likely a placeholder, but MPI may not be.
These can also include LC, service contracts, etc, so approximation at best
New TMT Tribrid from Thermo for #ASMS2026 (public info)
- IRMPD is the new UPVD
- IRMPD with TMT boosts reporter intensity by up to 240% without affecting quant
- New resolutions -> Down to 1k res, with an associated loss of signal, but at least it looks faster!
- Improved proton transfer reaction
- IRMPD is the new UPVD
- IRMPD with TMT boosts reporter intensity by up to 240% without affecting quant
- New resolutions -> Down to 1k res, with an associated loss of signal, but at least it looks faster!
- Improved proton transfer reaction
November 6, 2025 at 7:18 AM
New TMT Tribrid from Thermo for #ASMS2026 (public info)
- IRMPD is the new UPVD
- IRMPD with TMT boosts reporter intensity by up to 240% without affecting quant
- New resolutions -> Down to 1k res, with an associated loss of signal, but at least it looks faster!
- Improved proton transfer reaction
- IRMPD is the new UPVD
- IRMPD with TMT boosts reporter intensity by up to 240% without affecting quant
- New resolutions -> Down to 1k res, with an associated loss of signal, but at least it looks faster!
- Improved proton transfer reaction
A popular post on LinkedIn related to the reliability of current state-of-the-art instrumentation. #teammassspec
Related, anyone know how many more scientists are employed in marketing compared to R&D?
Related, anyone know how many more scientists are employed in marketing compared to R&D?
November 3, 2025 at 7:20 AM
A popular post on LinkedIn related to the reliability of current state-of-the-art instrumentation. #teammassspec
Related, anyone know how many more scientists are employed in marketing compared to R&D?
Related, anyone know how many more scientists are employed in marketing compared to R&D?
#glycotime #microsky
"host-cell directed genome-wide screen for host factors required for rhoptry discharge from Toxoplasma gondii, the causative agent of toxoplasmosis. The screen identified host N-glycosylation and cholesterol biosynthesis as pathways required for normal rhoptry discharge."
"host-cell directed genome-wide screen for host factors required for rhoptry discharge from Toxoplasma gondii, the causative agent of toxoplasmosis. The screen identified host N-glycosylation and cholesterol biosynthesis as pathways required for normal rhoptry discharge."
October 22, 2025 at 1:42 PM
#glycotime #microsky
"host-cell directed genome-wide screen for host factors required for rhoptry discharge from Toxoplasma gondii, the causative agent of toxoplasmosis. The screen identified host N-glycosylation and cholesterol biosynthesis as pathways required for normal rhoptry discharge."
"host-cell directed genome-wide screen for host factors required for rhoptry discharge from Toxoplasma gondii, the causative agent of toxoplasmosis. The screen identified host N-glycosylation and cholesterol biosynthesis as pathways required for normal rhoptry discharge."
New Sciex patent -> negative Electron Transfer Dissociation (nETD) on a ZenoTOF.
Looks like they're solidly aiming for the biopharma market on this next ZenoTOF (9600?), specifically, oligonucleotides.
patentscope.wipo.int/search/en/de...
Looks like they're solidly aiming for the biopharma market on this next ZenoTOF (9600?), specifically, oligonucleotides.
patentscope.wipo.int/search/en/de...
October 21, 2025 at 11:00 AM
New Sciex patent -> negative Electron Transfer Dissociation (nETD) on a ZenoTOF.
Looks like they're solidly aiming for the biopharma market on this next ZenoTOF (9600?), specifically, oligonucleotides.
patentscope.wipo.int/search/en/de...
Looks like they're solidly aiming for the biopharma market on this next ZenoTOF (9600?), specifically, oligonucleotides.
patentscope.wipo.int/search/en/de...
Sadly, I have to confirm that ChatGPT was poisoned by your earlier searches.
I tried the same prompt and it said they should use a NotATof mass analyser, which I made up as the name for the Astral.
Had a completely false backstory for it too!
I tried the same prompt and it said they should use a NotATof mass analyser, which I made up as the name for the Astral.
Had a completely false backstory for it too!
October 16, 2025 at 3:49 PM
Sadly, I have to confirm that ChatGPT was poisoned by your earlier searches.
I tried the same prompt and it said they should use a NotATof mass analyser, which I made up as the name for the Astral.
Had a completely false backstory for it too!
I tried the same prompt and it said they should use a NotATof mass analyser, which I made up as the name for the Astral.
Had a completely false backstory for it too!
Hey, it gets some things right!
October 16, 2025 at 2:31 PM
Hey, it gets some things right!
Anyone looking forward to purchasing an LC-MS co-developed by OpenAI?
October 16, 2025 at 1:57 PM
Anyone looking forward to purchasing an LC-MS co-developed by OpenAI?
Spec sheet is pretty barebones (technically not even a spec sheet) but the tech notes has more info:
October 13, 2025 at 11:19 AM
Spec sheet is pretty barebones (technically not even a spec sheet) but the tech notes has more info:
Wrapping up the 2025 Skyline Online Small Molecule course! Fantastic instructors and presenters, including Haley from the Baker lab with an insightful ion mobility crash course.
Great discussion and interaction from participants this year—looking forward to next year! #teammassspec
Great discussion and interaction from participants this year—looking forward to next year! #teammassspec
October 10, 2025 at 7:20 PM
Wrapping up the 2025 Skyline Online Small Molecule course! Fantastic instructors and presenters, including Haley from the Baker lab with an insightful ion mobility crash course.
Great discussion and interaction from participants this year—looking forward to next year! #teammassspec
Great discussion and interaction from participants this year—looking forward to next year! #teammassspec
Very excited to kick off the annual Skyline Online 2025 Small Molecule course with a keynote lecture by Gary Patti @gjpattij.bsky.social.
Showing how his lab and collaborators are advancing mass spectrometry to achieve parity with sequencing techs, supported by Skyline!
Showing how his lab and collaborators are advancing mass spectrometry to achieve parity with sequencing techs, supported by Skyline!
October 8, 2025 at 1:57 PM
Very excited to kick off the annual Skyline Online 2025 Small Molecule course with a keynote lecture by Gary Patti @gjpattij.bsky.social.
Showing how his lab and collaborators are advancing mass spectrometry to achieve parity with sequencing techs, supported by Skyline!
Showing how his lab and collaborators are advancing mass spectrometry to achieve parity with sequencing techs, supported by Skyline!
This is what LC-MS proteomics is up against.
Generation of a knock-out cell line + western blot VS targeted LC-MS proteomics.
Pretty sure one is cheaper, faster and more reproducible than the other.
Here's that protein from the GPMDB.
Generation of a knock-out cell line + western blot VS targeted LC-MS proteomics.
Pretty sure one is cheaper, faster and more reproducible than the other.
Here's that protein from the GPMDB.
October 3, 2025 at 11:03 AM
This is what LC-MS proteomics is up against.
Generation of a knock-out cell line + western blot VS targeted LC-MS proteomics.
Pretty sure one is cheaper, faster and more reproducible than the other.
Here's that protein from the GPMDB.
Generation of a knock-out cell line + western blot VS targeted LC-MS proteomics.
Pretty sure one is cheaper, faster and more reproducible than the other.
Here's that protein from the GPMDB.
Confirmed by Thermo marketing. So feel free to let your sales reps know that you want a custom Astral Zoom.
October 2, 2025 at 11:01 AM
Confirmed by Thermo marketing. So feel free to let your sales reps know that you want a custom Astral Zoom.
Without #2, a lower ion count is needed just to be sure that the full MS range is scanned, but with more accurate ion counts, you can go to the max S/N without losing ions on the edges.
This could also work for the Orbitrap Astral.
Bonus: DIAPASEF on Thermo - patentscope.wipo.int/search/en/de...
This could also work for the Orbitrap Astral.
Bonus: DIAPASEF on Thermo - patentscope.wipo.int/search/en/de...
September 28, 2025 at 1:25 PM
Without #2, a lower ion count is needed just to be sure that the full MS range is scanned, but with more accurate ion counts, you can go to the max S/N without losing ions on the edges.
This could also work for the Orbitrap Astral.
Bonus: DIAPASEF on Thermo - patentscope.wipo.int/search/en/de...
This could also work for the Orbitrap Astral.
Bonus: DIAPASEF on Thermo - patentscope.wipo.int/search/en/de...
A new Tribrid next year, suggest two patents made public simultaneously, representing improved full Orbitrap scan range and more accurate Orbitrap ion measurement:
1. C-trap improvement from 15x lowest mass, to 40x lowest mass
2. Using the LIT to count ions for accurate AGC on the Orbitrap
1. C-trap improvement from 15x lowest mass, to 40x lowest mass
2. Using the LIT to count ions for accurate AGC on the Orbitrap
September 28, 2025 at 1:18 PM
A new Tribrid next year, suggest two patents made public simultaneously, representing improved full Orbitrap scan range and more accurate Orbitrap ion measurement:
1. C-trap improvement from 15x lowest mass, to 40x lowest mass
2. Using the LIT to count ions for accurate AGC on the Orbitrap
1. C-trap improvement from 15x lowest mass, to 40x lowest mass
2. Using the LIT to count ions for accurate AGC on the Orbitrap
Rumour is that Sciex are currently the lowest priced top-tier MS right now, with the 8600 coming in around $1 million USD.
They should make their money back in other ways (software subscriptions in particular), but if you want the cheapest, single cell-level MS, the 8600 is probably it.
They should make their money back in other ways (software subscriptions in particular), but if you want the cheapest, single cell-level MS, the 8600 is probably it.
September 26, 2025 at 1:41 PM
Rumour is that Sciex are currently the lowest priced top-tier MS right now, with the 8600 coming in around $1 million USD.
They should make their money back in other ways (software subscriptions in particular), but if you want the cheapest, single cell-level MS, the 8600 is probably it.
They should make their money back in other ways (software subscriptions in particular), but if you want the cheapest, single cell-level MS, the 8600 is probably it.
Seen in the wild, another limited edition Astral Zoom.
This time with white trim and 40% brighter ion inlet (obviously caused by the trim).
This time with white trim and 40% brighter ion inlet (obviously caused by the trim).
September 25, 2025 at 2:37 PM
Seen in the wild, another limited edition Astral Zoom.
This time with white trim and 40% brighter ion inlet (obviously caused by the trim).
This time with white trim and 40% brighter ion inlet (obviously caused by the trim).
Pretty cool update from GlycoScape:
Sugar-specific visualisation, based on the sugar type. Should help for hypothesis generation since you can plot each sugar type and compare.
Sugar-specific visualisation, based on the sugar type. Should help for hypothesis generation since you can plot each sugar type and compare.
September 25, 2025 at 5:21 AM
Pretty cool update from GlycoScape:
Sugar-specific visualisation, based on the sugar type. Should help for hypothesis generation since you can plot each sugar type and compare.
Sugar-specific visualisation, based on the sugar type. Should help for hypothesis generation since you can plot each sugar type and compare.
September 22, 2025 at 7:42 PM
Boiling point, and what can be referred to as “Vapor pressure”.
Basically it’s a table that has the pressure and temperature, and you can see that as pressure decreases, boiling point decreases also.
E.g. at 40 mm Hg (5% atmosphere pressure), formic acid boils at room temp.
Basically it’s a table that has the pressure and temperature, and you can see that as pressure decreases, boiling point decreases also.
E.g. at 40 mm Hg (5% atmosphere pressure), formic acid boils at room temp.
September 17, 2025 at 2:10 PM
Boiling point, and what can be referred to as “Vapor pressure”.
Basically it’s a table that has the pressure and temperature, and you can see that as pressure decreases, boiling point decreases also.
E.g. at 40 mm Hg (5% atmosphere pressure), formic acid boils at room temp.
Basically it’s a table that has the pressure and temperature, and you can see that as pressure decreases, boiling point decreases also.
E.g. at 40 mm Hg (5% atmosphere pressure), formic acid boils at room temp.
A new Real time QC node (inspired by Bruker)?
September 16, 2025 at 2:37 AM
A new Real time QC node (inspired by Bruker)?
Adaptive RT only works with resonant CID (at the moment), but I'm curious why.
More specific MS2 for a given precursor?
Initial development was on Stellar but that instrument can do HCD and CID.
More specific MS2 for a given precursor?
Initial development was on Stellar but that instrument can do HCD and CID.
September 16, 2025 at 2:35 AM
Adaptive RT only works with resonant CID (at the moment), but I'm curious why.
More specific MS2 for a given precursor?
Initial development was on Stellar but that instrument can do HCD and CID.
More specific MS2 for a given precursor?
Initial development was on Stellar but that instrument can do HCD and CID.
If you have a Thermo Assend or Eclipse, the newest software update has enabled a few new features that weren't there when you bought them.
thermo.flexnetoperations.com/control/thmo...
thermo.flexnetoperations.com/control/thmo...
September 16, 2025 at 2:32 AM
If you have a Thermo Assend or Eclipse, the newest software update has enabled a few new features that weren't there when you bought them.
thermo.flexnetoperations.com/control/thmo...
thermo.flexnetoperations.com/control/thmo...
Nice to see our SUGA approach on PanoramaWeb. It hosts our raw files and Skyline assays, to improve data analysis reproducibility.
Quick and easy N-glycomics, suitable for those trying out glycan analysis without spending extra $ on anything except PNGase-F!
Paper: pubmed.ncbi.nlm.nih.gov/39978775/
Quick and easy N-glycomics, suitable for those trying out glycan analysis without spending extra $ on anything except PNGase-F!
Paper: pubmed.ncbi.nlm.nih.gov/39978775/
September 2, 2025 at 1:11 PM
Nice to see our SUGA approach on PanoramaWeb. It hosts our raw files and Skyline assays, to improve data analysis reproducibility.
Quick and easy N-glycomics, suitable for those trying out glycan analysis without spending extra $ on anything except PNGase-F!
Paper: pubmed.ncbi.nlm.nih.gov/39978775/
Quick and easy N-glycomics, suitable for those trying out glycan analysis without spending extra $ on anything except PNGase-F!
Paper: pubmed.ncbi.nlm.nih.gov/39978775/
Sialic acid acetylation is of increasing interest and GlyCombo is one such tool to find them♦️.
For an upcoming preprint (with @maiakelly.bsky.social at @proteaglyco.bsky.social), we've found a trick to ensure searching glycomics LC-MS data is as fast as possible⏩, even in complicated search spaces.
For an upcoming preprint (with @maiakelly.bsky.social at @proteaglyco.bsky.social), we've found a trick to ensure searching glycomics LC-MS data is as fast as possible⏩, even in complicated search spaces.
August 21, 2025 at 1:42 PM
Sialic acid acetylation is of increasing interest and GlyCombo is one such tool to find them♦️.
For an upcoming preprint (with @maiakelly.bsky.social at @proteaglyco.bsky.social), we've found a trick to ensure searching glycomics LC-MS data is as fast as possible⏩, even in complicated search spaces.
For an upcoming preprint (with @maiakelly.bsky.social at @proteaglyco.bsky.social), we've found a trick to ensure searching glycomics LC-MS data is as fast as possible⏩, even in complicated search spaces.