Brian Stevenson Ph.D. spirochete lab
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bstevensonlab.bsky.social
Brian Stevenson Ph.D. spirochete lab
@bstevensonlab.bsky.social
3K followers 2K following 740 posts
Mostly about bacterial gene/protein regulation & function, esp. with Borrelia burgdorferi and Lyme disease. Professor at U Kentucky, views are my own. He/him
Lab= http://microscopist.net/Stevenson_Lab.html
UK= https://medicine.uky.edu/users/bstev0
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bstevensonlab.bsky.social
Soooooo, lots of OspA protein in your lab’s floor dust?
November 10, 2025 at 7:57 PM
bstevensonlab.bsky.social
The answer may not be at the bottom of a margarita glass, but we should at least check
November 7, 2025 at 10:26 PM
bstevensonlab.bsky.social
Gac Is a Transcriptional Repressor of the Lyme Disease Spirochete's OspC Virulence-Associated Surface Protein

Castro-Padovani, Saylor, Husted, Krusenstjerna, Jusufovic, and Stevenson
2023, J Bacteriol, 205:e0044022.
doi: 10.1128/jb.00440-22
pubmed.ncbi.nlm.nih.gov/36920207/
#MicroSky
(A) Electrophoretic mobility shift assay (EMSA) of labeled Ip89 ospC operator, without (−) and with (+) cytoplasmic extract from strain B31. The asterisk indicates shifted probe that was present only when borrelial extract was included, indicating presence of a DNA-binding component. Unbound DNA is indicated by “free.” Some labeled DNA probe remained in the wells of the gel. (B) Cytoplasmic extract from strain B31 was incubated with Ip89 ospC operator DNA affixed to beads, eluted with increasing concentrations of NaCl, and eluates were separated by SDS-polyacrylamide gel electrophoresis. Lanes are labeled with the concentration of NaCl used for that elution. The arrow indicated a protein band of 30 to 35 kDa that eluted in the 0.3 and 0.5 mM NaCl washes. (C) Amino acid sequence of GyrA and Gac (the two potential initiation methionines of Gac are indicated by boldface and underlining). Mass spectrometry identified nine unique peptides that all mapped to the Gac ORF. Peptides identified with high confidence are shaded green, and those with lower confidence are red. (D) Predicted structures of B. burgdorferi Gac and GyrA proteins, as determined by AlphaFold (https://alphafold.ebi.ac.uk). Both are consistent with the solved structures of B. burgdorferi Gac and the N-terminal domain of E. coli GyrA (https://www.rcsb.org/structure/4ckk).
November 7, 2025 at 3:51 PM
bstevensonlab.bsky.social
Quantitative analyses of interactions between SpoVG and RNA/DNA
Saylor et al, 2023, Biochem Biophys Res Commun, 30:654:40-46.
doi: 10.1016/j.bbrc.2023.02.044
pubmed.ncbi.nlm.nih.gov/36889033/
#MicroSky
Ribbon diagram of SpoVG dimer structure
November 7, 2025 at 3:47 PM
bstevensonlab.bsky.social
Moot point, since study sections don’t currently exist!
a man in a suit and tie stands at a podium with the question " the question is moot " written above him
Alt: a man in a suit and tie stands at a podium with the question " the question is moot " written above him
media.tenor.com
November 3, 2025 at 3:08 PM
bstevensonlab.bsky.social
a cartoon character with a yellow backpack and the words six seven on the bottom
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media.tenor.com
November 3, 2025 at 4:26 AM
bstevensonlab.bsky.social
Wiley-Blackwell has the 1800s editions of Journal of the Royal Microscopical Society behind a paywall !
October 27, 2025 at 2:50 PM
bstevensonlab.bsky.social
Beautiful artwork! Thank you for sharing!
October 27, 2025 at 9:38 AM
bstevensonlab.bsky.social
When we fight, we fight together, not alone!
www.youtube.com/watch?v=sAa4...
Blackmore's Night - All For One (ZDF Fernsehgarten, Jul 20th, 2003)
YouTube video by Blackmore's Night
www.youtube.com
October 26, 2025 at 4:08 AM
bstevensonlab.bsky.social
Lovely work!
October 25, 2025 at 7:01 PM
bstevensonlab.bsky.social
Everything is edible at least once
October 25, 2025 at 6:38 PM
bstevensonlab.bsky.social
Congratulations to Jeff and crew!
bugs bunny is sitting at a table with a purse and stacks of money
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media.tenor.com
October 22, 2025 at 5:36 PM
bstevensonlab.bsky.social
Link is not functional
October 21, 2025 at 12:57 PM