Also proud of our lab members — Olga Vondálová Blanářová, Michaela Chorvátová, Nela Kolčáková, Hana Plešingerová & Pavlína Janovská — for their excellent poster presentations! 👏
See you at the next edition! @csac-cz.bsky.social

🤗 This work would not have been possible without the fantastic collaboration between our lab and K. Tripsianes’ lab at @ceitec.eu. Also, huge thanks to our funding sources — the Czech Science Foundation & the Ministry of Education, Youth and Sports. 👏

🧐In a nutshell: CK1-mediated multisite phosphorylation of Dishevelled dramatically alters its bulk electrostatics, promoting DVL intramolecular coupling between the positively charged cleft of the DEP domain and the adjacent phosphorylated disordered region.

Many thanks @chriswaudby.bsky.social , and also to @michaelranes.bsky.social and Lucie Wolf, for coming all the way to Brno and doing such an excellent job as examiners. 👏

Day 2 of our Milestone minisymposium at @ceitec.eu in Brno was packed with excellent talks by Lucie Wolf from Stephane Angers’ lab at @utoronto.ca, followed by @cajaneklab.bsky.social and Víťa as the closing speaker. 🔥

Big shout-out to @sumbalovakoledova.bsky.social, Marcela Buchtová, Alexandra Špirková & our PhD student @petrakompanikova.bsky.social, who also presented a poster on her Choroid Plexus development story, for organizing such an excellent meeting 🔥

And huge thanks for the support @biologists.bsky.social, @ceitec.eu, @uebscimuni.bsky.social, Milestone & CasInvent Pharma 🤗🎉

🤗 A huge thanks to the Czech Science Foundation and the National Infrastructure for Chemical Biology for supporting our work on CK1 δ/ε degraders! Stay tuned for more CK1 biology updates! 👩‍🔬

📌AH078 is a powerful tool for studying CK1 δ/ε biology in cells, enabling rapid depletion of both kinases—something challenging with KO systems due to cellular dependency on CK1 δ/ε activity!

👉 The PROTAC AH078 is characterized through in vitro and cellular assays, demonstrating on-target activity, selectivity, and a VHL-dependent mechanism of the CK1 degradation in cells.