Alessandro Galloni
@argalloni.bsky.social
Computational neuroscience postdoc in the Milstein Lab at Rutgers University, studying synaptic plasticity, bio-plausible deep learning / neuroAI, neuromorphic computing. Previously @ Francis Crick Institute & UCL
On the other hand, Rs compensation in Voltage Clamp is an active circuit that directly drives the cell voltage higher (so it's very important to get it right online!)
Because of the positive feedback loop (command voltage driven by recorded current), it is prone to unstable oscillations.
Because of the positive feedback loop (command voltage driven by recorded current), it is prone to unstable oscillations.
September 23, 2025 at 9:32 PM
On the other hand, Rs compensation in Voltage Clamp is an active circuit that directly drives the cell voltage higher (so it's very important to get it right online!)
Because of the positive feedback loop (command voltage driven by recorded current), it is prone to unstable oscillations.
Because of the positive feedback loop (command voltage driven by recorded current), it is prone to unstable oscillations.
For example, does bridge balance compensation affects how much current is being delivered to the cell?
Answer: no, bridge balance is a cosmetic DC correction to the recorded voltage (proportional to delivered current). It doesn't influence anything about the actual pipette/cell/injected current
Answer: no, bridge balance is a cosmetic DC correction to the recorded voltage (proportional to delivered current). It doesn't influence anything about the actual pipette/cell/injected current
September 23, 2025 at 9:32 PM
For example, does bridge balance compensation affects how much current is being delivered to the cell?
Answer: no, bridge balance is a cosmetic DC correction to the recorded voltage (proportional to delivered current). It doesn't influence anything about the actual pipette/cell/injected current
Answer: no, bridge balance is a cosmetic DC correction to the recorded voltage (proportional to delivered current). It doesn't influence anything about the actual pipette/cell/injected current
Many students (and practitioners!) get confused about how series resistance, pipette capacitance, and various electronic compensation circuits affect your recordings.
Now you can see for yourself in the simulator!
tinyurl.com/patch-clamp-...
Now you can see for yourself in the simulator!
tinyurl.com/patch-clamp-...
September 23, 2025 at 9:32 PM
Many students (and practitioners!) get confused about how series resistance, pipette capacitance, and various electronic compensation circuits affect your recordings.
Now you can see for yourself in the simulator!
tinyurl.com/patch-clamp-...
Now you can see for yourself in the simulator!
tinyurl.com/patch-clamp-...
Hey ephys friends! I've been teaching electrophysiology for a few years now, and this summer while teaching at CSHL I started building an interactive simulation of a full patch-clamp amplifier circuit to help explain electronics to my students.
Sharing it here in case anyone finds it useful!
Sharing it here in case anyone finds it useful!
September 23, 2025 at 9:32 PM
Hey ephys friends! I've been teaching electrophysiology for a few years now, and this summer while teaching at CSHL I started building an interactive simulation of a full patch-clamp amplifier circuit to help explain electronics to my students.
Sharing it here in case anyone finds it useful!
Sharing it here in case anyone finds it useful!
Is this neuron ok? 😂
April 9, 2025 at 10:13 PM
Is this neuron ok? 😂