I love the 10%/90% science-to-venting ratio in this tweet.

Ahhhh that makes sense, the halfway point sucks.

In my experience, the hardest part of teaching a biologist to code is uninstalling the three extra versions of python they have on their Mac (brew, conda, etc), and relearning basic crap that Mac obfuscates like "file paths".

I've never failed, when I try this. I've done it on Windows and Linux. I don't do anything fancy, I follow the default instructions. What goes wrong for you?

This press release is bizarre. We've had the iSIM and the SoRa as commercial products for almost a decade now.

How can you ignore a technique that's widely distributed and strictly superior? Do you just not know about it?

cc @tanner-fadero.bsky.social

That's why we use the alignment crane: thousands of dollars worth of 3d precision that detaches post-alignment, leaving only a solid steel post/clamp that's nearly incapable of drift.

I think my speakers aren't working, I can't hear the Benny Hill music?

Oh I think I get it - 10 fps acquisition, 250 fps playback?

What a great answer! That's way more photons than I would have guessed.

More photons than Alexa 647! Amazing.

(also, looks like 10 fps, not 250, right?)

There's about 10^5 photons in a single GFP [1]; how many do we estimate are in a single smURFP?

(I'm interested if smURFP is really more photostable than GFP, vs the smURFP cell here is just more densely tagged and imaged at a lower excitation rate)

[1] doi.org/10.1016/j.bb...

It will also have enormous advantages over light field, because it will be several orders of magnitude faster, and much higher resolution.

Light field is an inherently slow, low resolution technique, unfortunately. It's strange no one seems to notice this.

I know exactly how to do that inference. Hit me up for a zoom call.

(It's the same math as every other inverse problem in microscopy, btw).

Hey awesome!

How many pixels are in it?

(I know we both know this, but I'm hoping folks will stop using the correct but shibboleth-y terms "etendue"/"space-bandwidth product" and switch to using the more accessible term "how many pixels are in that lens")

doi.org/10.5281/zeno...

Illumination gives singlets and triplets in ~100-1 ratio. Bleaching is mostly from the long-lived (~ms) reactive triplet. Triplets also absorb light, which can be protective or destructive depending on the color and FP. Higher intensity gives more triplet absorption.

www.nature.com/articles/s41...

The hosts are friends of mine, it'll be at their place. It's a very tech-focused house with a lot of very driven people living (and working?) there.

Click the link and register to get the address.