Adam Steinbrenner
@adsteinbrenner.bsky.social
Associate Prof @UWBiology Seattle. How do plants recognize and resist pests 🐛? We study receptor function + evolution in plant innate immunity. steinbrennerlab.org
What about the caterpillars? We generated low expression (inr-2 allele) near-isogenic lines, but interestingly, In11 pretreatment still protected these plants from attack.
We conclude that insensitivity with common lab assays for PTI does not necessary correspond with resistance.
We conclude that insensitivity with common lab assays for PTI does not necessary correspond with resistance.
September 5, 2025 at 3:29 AM
What about the caterpillars? We generated low expression (inr-2 allele) near-isogenic lines, but interestingly, In11 pretreatment still protected these plants from attack.
We conclude that insensitivity with common lab assays for PTI does not necessary correspond with resistance.
We conclude that insensitivity with common lab assays for PTI does not necessary correspond with resistance.
Promoter alleles from different In11-reponsive or non-responsive varieties corresponded with strength driving luciferase expression in transient expression assays. Thus, low INR expression seems to mediate insensitivity to In11 in lab conditions.
September 5, 2025 at 3:29 AM
Promoter alleles from different In11-reponsive or non-responsive varieties corresponded with strength driving luciferase expression in transient expression assays. Thus, low INR expression seems to mediate insensitivity to In11 in lab conditions.
We found just a few insensitive accessions among hundreds of tested USDA-GRIN varieties. One variety was associated with lower expression of the INR gene. The low expression INR haplotype turned out to be locally prevalent in varieties from Jujuy province in Argentina.
September 5, 2025 at 3:29 AM
We found just a few insensitive accessions among hundreds of tested USDA-GRIN varieties. One variety was associated with lower expression of the INR gene. The low expression INR haplotype turned out to be locally prevalent in varieties from Jujuy province in Argentina.
More fruits of studying natural variation in common bean (Phaseolus vulgaris): an interesting genetic basis for insensitivity to caterpillars' In11 elicitor 🐛
Our new bioRxiv preprint from star technicians Brian Behnken and Wesley George below 🧵
www.biorxiv.org/content/10.1...
Our new bioRxiv preprint from star technicians Brian Behnken and Wesley George below 🧵
www.biorxiv.org/content/10.1...
September 5, 2025 at 3:29 AM
More fruits of studying natural variation in common bean (Phaseolus vulgaris): an interesting genetic basis for insensitivity to caterpillars' In11 elicitor 🐛
Our new bioRxiv preprint from star technicians Brian Behnken and Wesley George below 🧵
www.biorxiv.org/content/10.1...
Our new bioRxiv preprint from star technicians Brian Behnken and Wesley George below 🧵
www.biorxiv.org/content/10.1...
How do predatory wasps find their caterpillar prey?
In our new preprint on Biorxiv we show that the host plant's immune system detects herbivory to sound the alarm. 🌱🐛
See thread from @nataliagupa.bsky.social -- a surprising role for a plant PRR in indirect defense against herbivores.
In our new preprint on Biorxiv we show that the host plant's immune system detects herbivory to sound the alarm. 🌱🐛
See thread from @nataliagupa.bsky.social -- a surprising role for a plant PRR in indirect defense against herbivores.
August 2, 2025 at 4:04 PM
How do predatory wasps find their caterpillar prey?
In our new preprint on Biorxiv we show that the host plant's immune system detects herbivory to sound the alarm. 🌱🐛
See thread from @nataliagupa.bsky.social -- a surprising role for a plant PRR in indirect defense against herbivores.
In our new preprint on Biorxiv we show that the host plant's immune system detects herbivory to sound the alarm. 🌱🐛
See thread from @nataliagupa.bsky.social -- a surprising role for a plant PRR in indirect defense against herbivores.
Second, work from Eunyoung Chae on autoimmunity mediated by the NLR DM2 and DM3 "Dangerous mix".
CryoEM structures show a shift in enzyme hexamer stability to be the allele-specific trigger for DM2.
We highlight that Alphafold can't predict this structural shift.
www.cell.com/molecular-ce...
CryoEM structures show a shift in enzyme hexamer stability to be the allele-specific trigger for DM2.
We highlight that Alphafold can't predict this structural shift.
www.cell.com/molecular-ce...
July 17, 2025 at 5:03 PM
Second, work from Eunyoung Chae on autoimmunity mediated by the NLR DM2 and DM3 "Dangerous mix".
CryoEM structures show a shift in enzyme hexamer stability to be the allele-specific trigger for DM2.
We highlight that Alphafold can't predict this structural shift.
www.cell.com/molecular-ce...
CryoEM structures show a shift in enzyme hexamer stability to be the allele-specific trigger for DM2.
We highlight that Alphafold can't predict this structural shift.
www.cell.com/molecular-ce...
First, work from Chang-Bao Li and Chuanyou Li (Shandong Ag U) on regulation of SYR1 receptor.
The paralog SYR2 is an induced negative regulator w/ lower binding affinity.
@nataliagupa.bsky.social highlighted that the module is only found in Solanoideae
www.sciencedirect.com/science/arti...
The paralog SYR2 is an induced negative regulator w/ lower binding affinity.
@nataliagupa.bsky.social highlighted that the module is only found in Solanoideae
www.sciencedirect.com/science/arti...
July 17, 2025 at 5:03 PM
First, work from Chang-Bao Li and Chuanyou Li (Shandong Ag U) on regulation of SYR1 receptor.
The paralog SYR2 is an induced negative regulator w/ lower binding affinity.
@nataliagupa.bsky.social highlighted that the module is only found in Solanoideae
www.sciencedirect.com/science/arti...
The paralog SYR2 is an induced negative regulator w/ lower binding affinity.
@nataliagupa.bsky.social highlighted that the module is only found in Solanoideae
www.sciencedirect.com/science/arti...
See our latest work on an LRR-RLP receptor and plant-caterpillar interactions this Wed at #2025ISMPMI
The legume receptor INR attracts predatory wasps in the field!🍀🐛 💀
@nataliagupa.bsky.social presents in CS: Microbe-mediated impacts on plant-parasite interaction (we sneak in among the microbes)
The legume receptor INR attracts predatory wasps in the field!🍀🐛 💀
@nataliagupa.bsky.social presents in CS: Microbe-mediated impacts on plant-parasite interaction (we sneak in among the microbes)
July 15, 2025 at 6:52 PM
See our latest work on an LRR-RLP receptor and plant-caterpillar interactions this Wed at #2025ISMPMI
The legume receptor INR attracts predatory wasps in the field!🍀🐛 💀
@nataliagupa.bsky.social presents in CS: Microbe-mediated impacts on plant-parasite interaction (we sneak in among the microbes)
The legume receptor INR attracts predatory wasps in the field!🍀🐛 💀
@nataliagupa.bsky.social presents in CS: Microbe-mediated impacts on plant-parasite interaction (we sneak in among the microbes)
Love this Darwin quote on display at the Linnaean Society in London:
"Trust nothing to the memory, for the memory becomes a fickle guardian when one interesting object is succeeded by another still more interesting"
darwin-online.org.uk/content/fram...
"Trust nothing to the memory, for the memory becomes a fickle guardian when one interesting object is succeeded by another still more interesting"
darwin-online.org.uk/content/fram...
May 15, 2025 at 5:59 AM
Love this Darwin quote on display at the Linnaean Society in London:
"Trust nothing to the memory, for the memory becomes a fickle guardian when one interesting object is succeeded by another still more interesting"
darwin-online.org.uk/content/fram...
"Trust nothing to the memory, for the memory becomes a fickle guardian when one interesting object is succeeded by another still more interesting"
darwin-online.org.uk/content/fram...
We had a lab discussion of time management using concepts from the book "Make Time" -- how do we avoid "Busy Bandwagons" / "Infinity Pools" , and how do we pick and carry out a high-priority task each day? We listed some examples and strategies. Any other ideas?
December 4, 2024 at 7:17 PM
We had a lab discussion of time management using concepts from the book "Make Time" -- how do we avoid "Busy Bandwagons" / "Infinity Pools" , and how do we pick and carry out a high-priority task each day? We listed some examples and strategies. Any other ideas?
Reintroducing myself on Bluesky @adsteinbrenner.bsky.social -- I'm a plant biologist at UW Seattle (just recently made Associate Professor!)
We study the plant immune system with a special interest in recognition of chewing herbivores and host-pest molecular evolution.
We study the plant immune system with a special interest in recognition of chewing herbivores and host-pest molecular evolution.
November 18, 2024 at 11:20 PM
Reintroducing myself on Bluesky @adsteinbrenner.bsky.social -- I'm a plant biologist at UW Seattle (just recently made Associate Professor!)
We study the plant immune system with a special interest in recognition of chewing herbivores and host-pest molecular evolution.
We study the plant immune system with a special interest in recognition of chewing herbivores and host-pest molecular evolution.
I had exciting conversations and enjoyed the excellent talks. A very high quality conference overall -- thanks to the organizers Sarah O'Connor, Barbara Halkier, and Detlef Weigel. Prost!
April 15, 2024 at 9:28 AM
I had exciting conversations and enjoyed the excellent talks. A very high quality conference overall -- thanks to the organizers Sarah O'Connor, Barbara Halkier, and Detlef Weigel. Prost!
On biotic interactions:
- dissecting plasmodesmatal closure from PTI responses @pd_christine
- apparency as critical trait for Scaptomyza leaf miner choice @NKWhiteman
- applications of GWAS in local populations Andy Gloss, hiring soon @ UGA
@ADGloss
- dissecting plasmodesmatal closure from PTI responses @pd_christine
- apparency as critical trait for Scaptomyza leaf miner choice @NKWhiteman
- applications of GWAS in local populations Andy Gloss, hiring soon @ UGA
@ADGloss
April 15, 2024 at 9:27 AM
On biotic interactions:
- dissecting plasmodesmatal closure from PTI responses @pd_christine
- apparency as critical trait for Scaptomyza leaf miner choice @NKWhiteman
- applications of GWAS in local populations Andy Gloss, hiring soon @ UGA
@ADGloss
- dissecting plasmodesmatal closure from PTI responses @pd_christine
- apparency as critical trait for Scaptomyza leaf miner choice @NKWhiteman
- applications of GWAS in local populations Andy Gloss, hiring soon @ UGA
@ADGloss
We heard from the forefront of tool development:
- single cell multi-omics from Robin Buell (UGA)
- chromosome engineering from Michelle Rönspies (Karlsruhe)
- field-bulked EMS mutants for many species from Agnieszka Nielsen of Traitomic (supported by Carlsberg foundation🍻!)
- single cell multi-omics from Robin Buell (UGA)
- chromosome engineering from Michelle Rönspies (Karlsruhe)
- field-bulked EMS mutants for many species from Agnieszka Nielsen of Traitomic (supported by Carlsberg foundation🍻!)
April 15, 2024 at 9:25 AM
We heard from the forefront of tool development:
- single cell multi-omics from Robin Buell (UGA)
- chromosome engineering from Michelle Rönspies (Karlsruhe)
- field-bulked EMS mutants for many species from Agnieszka Nielsen of Traitomic (supported by Carlsberg foundation🍻!)
- single cell multi-omics from Robin Buell (UGA)
- chromosome engineering from Michelle Rönspies (Karlsruhe)
- field-bulked EMS mutants for many species from Agnieszka Nielsen of Traitomic (supported by Carlsberg foundation🍻!)
I really enjoyed this EMBL "Plant Genomes" symposium last week, a small conference with some of the very best talks I have heard on novel functions emerging at multiple phylogenetic scales 🌲🌿🌸🌾
Some highlights below 🧵
Some highlights below 🧵
April 15, 2024 at 9:23 AM
I really enjoyed this EMBL "Plant Genomes" symposium last week, a small conference with some of the very best talks I have heard on novel functions emerging at multiple phylogenetic scales 🌲🌿🌸🌾
Some highlights below 🧵
Some highlights below 🧵
Importantly, host targets monitored by CHS3-CSA1 ( plant kinases BAK1/BIR1/BIR3) promote the inactive dimer state, although it would be great to see more details presented about the potential NLR-kinase interfaces
February 16, 2024 at 2:28 AM
Importantly, host targets monitored by CHS3-CSA1 ( plant kinases BAK1/BIR1/BIR3) promote the inactive dimer state, although it would be great to see more details presented about the potential NLR-kinase interfaces
For the second, it was hard to imagine paired NLRs as homomeric resistosomes given the implications of shared promoter and observed RPS4-RRS1 heterodimer (Williams 2014 24744375). The current work helps understand the transition to a large mw complex, a putative mixed tetramer
February 16, 2024 at 2:27 AM
For the second, it was hard to imagine paired NLRs as homomeric resistosomes given the implications of shared promoter and observed RPS4-RRS1 heterodimer (Williams 2014 24744375). The current work helps understand the transition to a large mw complex, a putative mixed tetramer
For the first, PvPGIP2 was the first plant leucine-rich repeat protein with a crystal structure (template for many PRR/NLR homology models!)
Amazing to see a surprising mechanism -- beyond inhibition of PG enzymes, PvPGIP2 promotes formation of long-oligogalacturonide elicitors
Amazing to see a surprising mechanism -- beyond inhibition of PG enzymes, PvPGIP2 promotes formation of long-oligogalacturonide elicitors
February 16, 2024 at 2:27 AM
For the first, PvPGIP2 was the first plant leucine-rich repeat protein with a crystal structure (template for many PRR/NLR homology models!)
Amazing to see a surprising mechanism -- beyond inhibition of PG enzymes, PvPGIP2 promotes formation of long-oligogalacturonide elicitors
Amazing to see a surprising mechanism -- beyond inhibition of PG enzymes, PvPGIP2 promotes formation of long-oligogalacturonide elicitors
Fantastic new work on volatile-induced responses in Arabidopsis.
- Initial green leafy volatile (GLV) perception occurs in guard cells (rapid pGC1::GCaMP3 response)
- Ca2+ increase is specific to C6 aldehydes but not other GLVs/terpenes/MeJA)
www.nature.com/articles/s41...
- Initial green leafy volatile (GLV) perception occurs in guard cells (rapid pGC1::GCaMP3 response)
- Ca2+ increase is specific to C6 aldehydes but not other GLVs/terpenes/MeJA)
www.nature.com/articles/s41...
February 2, 2024 at 7:43 PM
Fantastic new work on volatile-induced responses in Arabidopsis.
- Initial green leafy volatile (GLV) perception occurs in guard cells (rapid pGC1::GCaMP3 response)
- Ca2+ increase is specific to C6 aldehydes but not other GLVs/terpenes/MeJA)
www.nature.com/articles/s41...
- Initial green leafy volatile (GLV) perception occurs in guard cells (rapid pGC1::GCaMP3 response)
- Ca2+ increase is specific to C6 aldehydes but not other GLVs/terpenes/MeJA)
www.nature.com/articles/s41...
Just heard of another @NSF GRFP rejection of a basic plant biology research proposal It is crazy to disqualify students based on highly subjective categories of "ineligible research"
www.nsf.gov/pubs/2023/ns...
www.nsf.gov/pubs/2023/ns...
December 11, 2023 at 5:44 PM
Just heard of another @NSF GRFP rejection of a basic plant biology research proposal It is crazy to disqualify students based on highly subjective categories of "ineligible research"
www.nsf.gov/pubs/2023/ns...
www.nsf.gov/pubs/2023/ns...
A USDA Economic Information Bulletin from June 2023: "Concentration and Competition in U.S. Agribusiness"
Market share for top 5 firms is 84% for corn and 79% for soybean. Massive changes in two decades since major mergers began
www.ers.usda.gov/webdocs/publ...
Market share for top 5 firms is 84% for corn and 79% for soybean. Massive changes in two decades since major mergers began
www.ers.usda.gov/webdocs/publ...
December 5, 2023 at 12:06 AM
A USDA Economic Information Bulletin from June 2023: "Concentration and Competition in U.S. Agribusiness"
Market share for top 5 firms is 84% for corn and 79% for soybean. Massive changes in two decades since major mergers began
www.ers.usda.gov/webdocs/publ...
Market share for top 5 firms is 84% for corn and 79% for soybean. Massive changes in two decades since major mergers began
www.ers.usda.gov/webdocs/publ...
Attempt #3. (A threadreader style webpage that was compatible with bluesky preview images would be ideal!)
threadreaderapp.com/thread/16960...
threadreaderapp.com/thread/16960...
September 1, 2023 at 11:50 PM
Attempt #3. (A threadreader style webpage that was compatible with bluesky preview images would be ideal!)
threadreaderapp.com/thread/16960...
threadreaderapp.com/thread/16960...